Cargando…

Morphology Characterization, Molecular Phylogeny, and Pathogenicity of Diaporthe passifloricola on Citrus reticulata cv. Nanfengmiju in Jiangxi Province, China

The Nanfengmiju (Citrus reticulata cv. Nanfengmiju), a high-quality local variety of mandarin, is one of the major fruit crops in Jiangxi Province, China. Citrus melanose and stem-end rot, two common fungal diseases of Nanfengmiju, are both caused by Diaporthe spp. (syn. Phomopsis spp.). Identificat...

Descripción completa

Detalles Bibliográficos
Autores principales: Chaisiri, Chingchai, Liu, Xiang-Yu, Yin, Wei-Xiao, Luo, Chao-Xi, Lin, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911537/
https://www.ncbi.nlm.nih.gov/pubmed/33498730
http://dx.doi.org/10.3390/plants10020218
_version_ 1783656365096435712
author Chaisiri, Chingchai
Liu, Xiang-Yu
Yin, Wei-Xiao
Luo, Chao-Xi
Lin, Yang
author_facet Chaisiri, Chingchai
Liu, Xiang-Yu
Yin, Wei-Xiao
Luo, Chao-Xi
Lin, Yang
author_sort Chaisiri, Chingchai
collection PubMed
description The Nanfengmiju (Citrus reticulata cv. Nanfengmiju), a high-quality local variety of mandarin, is one of the major fruit crops in Jiangxi Province, China. Citrus melanose and stem-end rot, two common fungal diseases of Nanfengmiju, are both caused by Diaporthe spp. (syn. Phomopsis spp.). Identification of the Diaporthe species is essential for epidemiological studies, quarantine measures, and management of diseases caused by these fungi. Melanose disease was observed on Nanfengmiju fruit in Jiangxi Province of China in 2016. Based on morphological characterization and multi-locus phylogenetic analyses, three out of 39 isolates from diseased samples were identified as D. passifloricola. Since these three isolates did not cause melanose on citrus fruit in the pathogenicity tests, they were presumed to be endophytic fungi present in the diseased tissues. However, our results indicate that D. passifloricola may persist as a symptom-less endophyte in the peel of citrus fruit, yet it may cause stem-end if it invades the stem end during fruit storage. To the best of our knowledge, this is the first report of D. passifloricola as the causal agent of the stem-end rot disease in Citrus reticulata cv. Nanfengmiju.
format Online
Article
Text
id pubmed-7911537
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79115372021-02-28 Morphology Characterization, Molecular Phylogeny, and Pathogenicity of Diaporthe passifloricola on Citrus reticulata cv. Nanfengmiju in Jiangxi Province, China Chaisiri, Chingchai Liu, Xiang-Yu Yin, Wei-Xiao Luo, Chao-Xi Lin, Yang Plants (Basel) Brief Report The Nanfengmiju (Citrus reticulata cv. Nanfengmiju), a high-quality local variety of mandarin, is one of the major fruit crops in Jiangxi Province, China. Citrus melanose and stem-end rot, two common fungal diseases of Nanfengmiju, are both caused by Diaporthe spp. (syn. Phomopsis spp.). Identification of the Diaporthe species is essential for epidemiological studies, quarantine measures, and management of diseases caused by these fungi. Melanose disease was observed on Nanfengmiju fruit in Jiangxi Province of China in 2016. Based on morphological characterization and multi-locus phylogenetic analyses, three out of 39 isolates from diseased samples were identified as D. passifloricola. Since these three isolates did not cause melanose on citrus fruit in the pathogenicity tests, they were presumed to be endophytic fungi present in the diseased tissues. However, our results indicate that D. passifloricola may persist as a symptom-less endophyte in the peel of citrus fruit, yet it may cause stem-end if it invades the stem end during fruit storage. To the best of our knowledge, this is the first report of D. passifloricola as the causal agent of the stem-end rot disease in Citrus reticulata cv. Nanfengmiju. MDPI 2021-01-23 /pmc/articles/PMC7911537/ /pubmed/33498730 http://dx.doi.org/10.3390/plants10020218 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Chaisiri, Chingchai
Liu, Xiang-Yu
Yin, Wei-Xiao
Luo, Chao-Xi
Lin, Yang
Morphology Characterization, Molecular Phylogeny, and Pathogenicity of Diaporthe passifloricola on Citrus reticulata cv. Nanfengmiju in Jiangxi Province, China
title Morphology Characterization, Molecular Phylogeny, and Pathogenicity of Diaporthe passifloricola on Citrus reticulata cv. Nanfengmiju in Jiangxi Province, China
title_full Morphology Characterization, Molecular Phylogeny, and Pathogenicity of Diaporthe passifloricola on Citrus reticulata cv. Nanfengmiju in Jiangxi Province, China
title_fullStr Morphology Characterization, Molecular Phylogeny, and Pathogenicity of Diaporthe passifloricola on Citrus reticulata cv. Nanfengmiju in Jiangxi Province, China
title_full_unstemmed Morphology Characterization, Molecular Phylogeny, and Pathogenicity of Diaporthe passifloricola on Citrus reticulata cv. Nanfengmiju in Jiangxi Province, China
title_short Morphology Characterization, Molecular Phylogeny, and Pathogenicity of Diaporthe passifloricola on Citrus reticulata cv. Nanfengmiju in Jiangxi Province, China
title_sort morphology characterization, molecular phylogeny, and pathogenicity of diaporthe passifloricola on citrus reticulata cv. nanfengmiju in jiangxi province, china
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911537/
https://www.ncbi.nlm.nih.gov/pubmed/33498730
http://dx.doi.org/10.3390/plants10020218
work_keys_str_mv AT chaisirichingchai morphologycharacterizationmolecularphylogenyandpathogenicityofdiaporthepassifloricolaoncitrusreticulatacvnanfengmijuinjiangxiprovincechina
AT liuxiangyu morphologycharacterizationmolecularphylogenyandpathogenicityofdiaporthepassifloricolaoncitrusreticulatacvnanfengmijuinjiangxiprovincechina
AT yinweixiao morphologycharacterizationmolecularphylogenyandpathogenicityofdiaporthepassifloricolaoncitrusreticulatacvnanfengmijuinjiangxiprovincechina
AT luochaoxi morphologycharacterizationmolecularphylogenyandpathogenicityofdiaporthepassifloricolaoncitrusreticulatacvnanfengmijuinjiangxiprovincechina
AT linyang morphologycharacterizationmolecularphylogenyandpathogenicityofdiaporthepassifloricolaoncitrusreticulatacvnanfengmijuinjiangxiprovincechina