Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro

SARS-CoV-2 is a newly emerging virus that currently lacks curative treatments. Lactoferrin (LF) is a naturally occurring non-toxic glycoprotein with broad-spectrum antiviral, immunomodulatory and anti-inflammatory effects. In this study, we assessed the potential of LF in the prevention of SARS-CoV-...

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Autores principales: Salaris, Claudio, Scarpa, Melania, Elli, Marina, Bertolini, Alice, Guglielmetti, Simone, Pregliasco, Fabrizio, Blandizzi, Corrado, Brun, Paola, Castagliuolo, Ignazio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911668/
https://www.ncbi.nlm.nih.gov/pubmed/33498631
http://dx.doi.org/10.3390/nu13020328
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author Salaris, Claudio
Scarpa, Melania
Elli, Marina
Bertolini, Alice
Guglielmetti, Simone
Pregliasco, Fabrizio
Blandizzi, Corrado
Brun, Paola
Castagliuolo, Ignazio
author_facet Salaris, Claudio
Scarpa, Melania
Elli, Marina
Bertolini, Alice
Guglielmetti, Simone
Pregliasco, Fabrizio
Blandizzi, Corrado
Brun, Paola
Castagliuolo, Ignazio
author_sort Salaris, Claudio
collection PubMed
description SARS-CoV-2 is a newly emerging virus that currently lacks curative treatments. Lactoferrin (LF) is a naturally occurring non-toxic glycoprotein with broad-spectrum antiviral, immunomodulatory and anti-inflammatory effects. In this study, we assessed the potential of LF in the prevention of SARS-CoV-2 infection in vitro. Antiviral immune response gene expression was analyzed by qRT-PCR in uninfected Caco-2 intestinal epithelial cells treated with LF. An infection assay for SARS-CoV-2 was performed in Caco-2 cells treated or not with LF. SARS-CoV-2 titer was determined by qRT-PCR, plaque assay and immunostaining. Inflammatory and anti-inflammatory cytokine production was determined by qRT-PCR. LF significantly induced the expression of IFNA1, IFNB1, TLR3, TLR7, IRF3, IRF7 and MAVS genes. Furthermore, LF partially inhibited SARS-CoV-2 infection and replication in Caco-2 intestinal epithelial cells. Our in vitro data support LF as an immune modulator of the antiviral immune response with moderate effects against SARS-CoV-2 infection.
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spelling pubmed-79116682021-02-28 Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro Salaris, Claudio Scarpa, Melania Elli, Marina Bertolini, Alice Guglielmetti, Simone Pregliasco, Fabrizio Blandizzi, Corrado Brun, Paola Castagliuolo, Ignazio Nutrients Article SARS-CoV-2 is a newly emerging virus that currently lacks curative treatments. Lactoferrin (LF) is a naturally occurring non-toxic glycoprotein with broad-spectrum antiviral, immunomodulatory and anti-inflammatory effects. In this study, we assessed the potential of LF in the prevention of SARS-CoV-2 infection in vitro. Antiviral immune response gene expression was analyzed by qRT-PCR in uninfected Caco-2 intestinal epithelial cells treated with LF. An infection assay for SARS-CoV-2 was performed in Caco-2 cells treated or not with LF. SARS-CoV-2 titer was determined by qRT-PCR, plaque assay and immunostaining. Inflammatory and anti-inflammatory cytokine production was determined by qRT-PCR. LF significantly induced the expression of IFNA1, IFNB1, TLR3, TLR7, IRF3, IRF7 and MAVS genes. Furthermore, LF partially inhibited SARS-CoV-2 infection and replication in Caco-2 intestinal epithelial cells. Our in vitro data support LF as an immune modulator of the antiviral immune response with moderate effects against SARS-CoV-2 infection. MDPI 2021-01-23 /pmc/articles/PMC7911668/ /pubmed/33498631 http://dx.doi.org/10.3390/nu13020328 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Salaris, Claudio
Scarpa, Melania
Elli, Marina
Bertolini, Alice
Guglielmetti, Simone
Pregliasco, Fabrizio
Blandizzi, Corrado
Brun, Paola
Castagliuolo, Ignazio
Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro
title Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro
title_full Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro
title_fullStr Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro
title_full_unstemmed Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro
title_short Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro
title_sort protective effects of lactoferrin against sars-cov-2 infection in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911668/
https://www.ncbi.nlm.nih.gov/pubmed/33498631
http://dx.doi.org/10.3390/nu13020328
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