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Investigation of the Cellular Effects of Beta- Cyclodextrin Derivatives on Caco-2 Intestinal Epithelial Cells
Cyclodextrins are widely used excipients for increasing water-solubility, delivery and bioavailability of lipophilic drugs. By using fluorescent cyclodextrin derivatives, we showed previously that cyclodextrins are able to enter Caco-2 intestinal cells by endocytosis, but the influence of different...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911713/ https://www.ncbi.nlm.nih.gov/pubmed/33504045 http://dx.doi.org/10.3390/pharmaceutics13020157 |
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author | Rusznyák, Ágnes Malanga, Milo Fenyvesi, Éva Szente, Lajos Váradi, Judit Bácskay, Ildikó Vecsernyés, Miklós Vasvári, Gábor Haimhoffer, Ádám Fehér, Pálma Ujhelyi, Zoltán Nagy Jr., Béla Fejes, Zsolt Fenyvesi, Ferenc |
author_facet | Rusznyák, Ágnes Malanga, Milo Fenyvesi, Éva Szente, Lajos Váradi, Judit Bácskay, Ildikó Vecsernyés, Miklós Vasvári, Gábor Haimhoffer, Ádám Fehér, Pálma Ujhelyi, Zoltán Nagy Jr., Béla Fejes, Zsolt Fenyvesi, Ferenc |
author_sort | Rusznyák, Ágnes |
collection | PubMed |
description | Cyclodextrins are widely used excipients for increasing water-solubility, delivery and bioavailability of lipophilic drugs. By using fluorescent cyclodextrin derivatives, we showed previously that cyclodextrins are able to enter Caco-2 intestinal cells by endocytosis, but the influence of different fluorescent labeling on the same cyclodextrin derivative has not been studied. The consequences of the cellular internalization of cyclodextrins have not been revealed yet either. The aims of this study were to compare the cellular internalization of fluorescein- and rhodamine-labeled (2-hydroxypropyl)-, (HPBCD) and randommethyl-β-cyclodextrins (RAMEB) and to investigate the intracellular effects of these derivatives on Caco-2 cells. Stimulation of the NF-kappa B pathway and autophagy and localization of these derivatives in lysosomes were tested. The endocytosis of these derivatives was examined by fluorescence microscopy and flow cytometry. Both fluorescein- and rhodamine-labeled derivatives entered the cells, therefore the type of the fluorescent labeling did not influence their internalization. Cyclodextrin pretreatment did not activate the translocation of the p65 subunit of the NF-kappa B heterodimer into the cell nuclei from the cytoplasm. After HPBCD or RAMEB treatment, formation of the autophagosomes did not increase compared to the control sample and at the same time these derivatives could be detected in lysosomes after internalization. |
format | Online Article Text |
id | pubmed-7911713 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79117132021-02-28 Investigation of the Cellular Effects of Beta- Cyclodextrin Derivatives on Caco-2 Intestinal Epithelial Cells Rusznyák, Ágnes Malanga, Milo Fenyvesi, Éva Szente, Lajos Váradi, Judit Bácskay, Ildikó Vecsernyés, Miklós Vasvári, Gábor Haimhoffer, Ádám Fehér, Pálma Ujhelyi, Zoltán Nagy Jr., Béla Fejes, Zsolt Fenyvesi, Ferenc Pharmaceutics Article Cyclodextrins are widely used excipients for increasing water-solubility, delivery and bioavailability of lipophilic drugs. By using fluorescent cyclodextrin derivatives, we showed previously that cyclodextrins are able to enter Caco-2 intestinal cells by endocytosis, but the influence of different fluorescent labeling on the same cyclodextrin derivative has not been studied. The consequences of the cellular internalization of cyclodextrins have not been revealed yet either. The aims of this study were to compare the cellular internalization of fluorescein- and rhodamine-labeled (2-hydroxypropyl)-, (HPBCD) and randommethyl-β-cyclodextrins (RAMEB) and to investigate the intracellular effects of these derivatives on Caco-2 cells. Stimulation of the NF-kappa B pathway and autophagy and localization of these derivatives in lysosomes were tested. The endocytosis of these derivatives was examined by fluorescence microscopy and flow cytometry. Both fluorescein- and rhodamine-labeled derivatives entered the cells, therefore the type of the fluorescent labeling did not influence their internalization. Cyclodextrin pretreatment did not activate the translocation of the p65 subunit of the NF-kappa B heterodimer into the cell nuclei from the cytoplasm. After HPBCD or RAMEB treatment, formation of the autophagosomes did not increase compared to the control sample and at the same time these derivatives could be detected in lysosomes after internalization. MDPI 2021-01-25 /pmc/articles/PMC7911713/ /pubmed/33504045 http://dx.doi.org/10.3390/pharmaceutics13020157 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rusznyák, Ágnes Malanga, Milo Fenyvesi, Éva Szente, Lajos Váradi, Judit Bácskay, Ildikó Vecsernyés, Miklós Vasvári, Gábor Haimhoffer, Ádám Fehér, Pálma Ujhelyi, Zoltán Nagy Jr., Béla Fejes, Zsolt Fenyvesi, Ferenc Investigation of the Cellular Effects of Beta- Cyclodextrin Derivatives on Caco-2 Intestinal Epithelial Cells |
title | Investigation of the Cellular Effects of Beta- Cyclodextrin Derivatives on Caco-2 Intestinal Epithelial Cells |
title_full | Investigation of the Cellular Effects of Beta- Cyclodextrin Derivatives on Caco-2 Intestinal Epithelial Cells |
title_fullStr | Investigation of the Cellular Effects of Beta- Cyclodextrin Derivatives on Caco-2 Intestinal Epithelial Cells |
title_full_unstemmed | Investigation of the Cellular Effects of Beta- Cyclodextrin Derivatives on Caco-2 Intestinal Epithelial Cells |
title_short | Investigation of the Cellular Effects of Beta- Cyclodextrin Derivatives on Caco-2 Intestinal Epithelial Cells |
title_sort | investigation of the cellular effects of beta- cyclodextrin derivatives on caco-2 intestinal epithelial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911713/ https://www.ncbi.nlm.nih.gov/pubmed/33504045 http://dx.doi.org/10.3390/pharmaceutics13020157 |
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