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Living Lithic and Sublithic Bacterial Communities in Namibian Drylands
Dryland xeric conditions exert a deterministic effect on microbial communities, forcing life into refuge niches. Deposited rocks can form a lithic niche for microorganisms in desert regions. Mineral weathering is a key process in soil formation and the importance of microbial-driven mineral weatheri...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911874/ https://www.ncbi.nlm.nih.gov/pubmed/33498742 http://dx.doi.org/10.3390/microorganisms9020235 |
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author | Genderjahn, Steffi Lewin, Simon Horn, Fabian Schleicher, Anja M. Mangelsdorf, Kai Wagner, Dirk |
author_facet | Genderjahn, Steffi Lewin, Simon Horn, Fabian Schleicher, Anja M. Mangelsdorf, Kai Wagner, Dirk |
author_sort | Genderjahn, Steffi |
collection | PubMed |
description | Dryland xeric conditions exert a deterministic effect on microbial communities, forcing life into refuge niches. Deposited rocks can form a lithic niche for microorganisms in desert regions. Mineral weathering is a key process in soil formation and the importance of microbial-driven mineral weathering for nutrient extraction is increasingly accepted. Advances in geobiology provide insight into the interactions between microorganisms and minerals that play an important role in weathering processes. In this study, we present the examination of the microbial diversity in dryland rocks from the Tsauchab River banks in Namibia. We paired culture-independent 16S rRNA gene amplicon sequencing with culture-dependent (isolation of bacteria) techniques to assess the community structure and diversity patterns. Bacteria isolated from dryland rocks are typical of xeric environments and are described as being involved in rock weathering processes. For the first time, we extracted extra- and intracellular DNA from rocks to enhance our understanding of potentially rock-weathering microorganisms. We compared the microbial community structure in different rock types (limestone, quartz-rich sandstone and quartz-rich shale) with adjacent soils below the rocks. Our results indicate differences in the living lithic and sublithic microbial communities. |
format | Online Article Text |
id | pubmed-7911874 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79118742021-02-28 Living Lithic and Sublithic Bacterial Communities in Namibian Drylands Genderjahn, Steffi Lewin, Simon Horn, Fabian Schleicher, Anja M. Mangelsdorf, Kai Wagner, Dirk Microorganisms Article Dryland xeric conditions exert a deterministic effect on microbial communities, forcing life into refuge niches. Deposited rocks can form a lithic niche for microorganisms in desert regions. Mineral weathering is a key process in soil formation and the importance of microbial-driven mineral weathering for nutrient extraction is increasingly accepted. Advances in geobiology provide insight into the interactions between microorganisms and minerals that play an important role in weathering processes. In this study, we present the examination of the microbial diversity in dryland rocks from the Tsauchab River banks in Namibia. We paired culture-independent 16S rRNA gene amplicon sequencing with culture-dependent (isolation of bacteria) techniques to assess the community structure and diversity patterns. Bacteria isolated from dryland rocks are typical of xeric environments and are described as being involved in rock weathering processes. For the first time, we extracted extra- and intracellular DNA from rocks to enhance our understanding of potentially rock-weathering microorganisms. We compared the microbial community structure in different rock types (limestone, quartz-rich sandstone and quartz-rich shale) with adjacent soils below the rocks. Our results indicate differences in the living lithic and sublithic microbial communities. MDPI 2021-01-23 /pmc/articles/PMC7911874/ /pubmed/33498742 http://dx.doi.org/10.3390/microorganisms9020235 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Genderjahn, Steffi Lewin, Simon Horn, Fabian Schleicher, Anja M. Mangelsdorf, Kai Wagner, Dirk Living Lithic and Sublithic Bacterial Communities in Namibian Drylands |
title | Living Lithic and Sublithic Bacterial Communities in Namibian Drylands |
title_full | Living Lithic and Sublithic Bacterial Communities in Namibian Drylands |
title_fullStr | Living Lithic and Sublithic Bacterial Communities in Namibian Drylands |
title_full_unstemmed | Living Lithic and Sublithic Bacterial Communities in Namibian Drylands |
title_short | Living Lithic and Sublithic Bacterial Communities in Namibian Drylands |
title_sort | living lithic and sublithic bacterial communities in namibian drylands |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911874/ https://www.ncbi.nlm.nih.gov/pubmed/33498742 http://dx.doi.org/10.3390/microorganisms9020235 |
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