Intracytoplasmic Sperm Injection in Cattle

Intracytoplasmic sperm injection (ICSI) involves the microinjection of sperm into a matured oocyte. Although this reproductive technology is successfully used in humans and many animal species, the efficiency of this procedure is low in the bovine species mainly due to failed oocyte activation follo...

Descripción completa

Detalles Bibliográficos
Autores principales: Unnikrishnan, Veena, Kastelic, John, Thundathil, Jacob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911995/
https://www.ncbi.nlm.nih.gov/pubmed/33572865
http://dx.doi.org/10.3390/genes12020198
_version_ 1783656472945623040
author Unnikrishnan, Veena
Kastelic, John
Thundathil, Jacob
author_facet Unnikrishnan, Veena
Kastelic, John
Thundathil, Jacob
author_sort Unnikrishnan, Veena
collection PubMed
description Intracytoplasmic sperm injection (ICSI) involves the microinjection of sperm into a matured oocyte. Although this reproductive technology is successfully used in humans and many animal species, the efficiency of this procedure is low in the bovine species mainly due to failed oocyte activation following sperm microinjection. This review discusses various reasons for the low efficiency of ICSI in cattle, potential solutions, and future directions for research in this area, emphasizing the contributions of testis-specific isoforms of Na/K-ATPase (ATP1A4) and phospholipase C zeta (PLC ζ). Improving the efficiency of bovine ICSI would benefit the cattle breeding industries by effectively utilizing semen from elite sires at their earliest possible age.
format Online
Article
Text
id pubmed-7911995
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79119952021-02-28 Intracytoplasmic Sperm Injection in Cattle Unnikrishnan, Veena Kastelic, John Thundathil, Jacob Genes (Basel) Review Intracytoplasmic sperm injection (ICSI) involves the microinjection of sperm into a matured oocyte. Although this reproductive technology is successfully used in humans and many animal species, the efficiency of this procedure is low in the bovine species mainly due to failed oocyte activation following sperm microinjection. This review discusses various reasons for the low efficiency of ICSI in cattle, potential solutions, and future directions for research in this area, emphasizing the contributions of testis-specific isoforms of Na/K-ATPase (ATP1A4) and phospholipase C zeta (PLC ζ). Improving the efficiency of bovine ICSI would benefit the cattle breeding industries by effectively utilizing semen from elite sires at their earliest possible age. MDPI 2021-01-29 /pmc/articles/PMC7911995/ /pubmed/33572865 http://dx.doi.org/10.3390/genes12020198 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Unnikrishnan, Veena
Kastelic, John
Thundathil, Jacob
Intracytoplasmic Sperm Injection in Cattle
title Intracytoplasmic Sperm Injection in Cattle
title_full Intracytoplasmic Sperm Injection in Cattle
title_fullStr Intracytoplasmic Sperm Injection in Cattle
title_full_unstemmed Intracytoplasmic Sperm Injection in Cattle
title_short Intracytoplasmic Sperm Injection in Cattle
title_sort intracytoplasmic sperm injection in cattle
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911995/
https://www.ncbi.nlm.nih.gov/pubmed/33572865
http://dx.doi.org/10.3390/genes12020198
work_keys_str_mv AT unnikrishnanveena intracytoplasmicsperminjectionincattle
AT kastelicjohn intracytoplasmicsperminjectionincattle
AT thundathiljacob intracytoplasmicsperminjectionincattle

Ejemplares similares