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Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle

The expression of glypicans in different hair follicle (HF) compartments and their potential roles during hair shaft growth are still poorly understood. Heparan sulfate proteoglycan (HSPG) distribution in HFs is classically investigated by conventional histology, biochemical analysis, and immunohist...

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Autores principales: Colin-Pierre, Charlie, Untereiner, Valérie, Sockalingum, Ganesh D., Berthélémy, Nicolas, Danoux, Louis, Bardey, Vincent, Mine, Solène, Jeanmaire, Christine, Ramont, Laurent, Brézillon, Stéphane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912031/
https://www.ncbi.nlm.nih.gov/pubmed/33573119
http://dx.doi.org/10.3390/biom11020192
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author Colin-Pierre, Charlie
Untereiner, Valérie
Sockalingum, Ganesh D.
Berthélémy, Nicolas
Danoux, Louis
Bardey, Vincent
Mine, Solène
Jeanmaire, Christine
Ramont, Laurent
Brézillon, Stéphane
author_facet Colin-Pierre, Charlie
Untereiner, Valérie
Sockalingum, Ganesh D.
Berthélémy, Nicolas
Danoux, Louis
Bardey, Vincent
Mine, Solène
Jeanmaire, Christine
Ramont, Laurent
Brézillon, Stéphane
author_sort Colin-Pierre, Charlie
collection PubMed
description The expression of glypicans in different hair follicle (HF) compartments and their potential roles during hair shaft growth are still poorly understood. Heparan sulfate proteoglycan (HSPG) distribution in HFs is classically investigated by conventional histology, biochemical analysis, and immunohistochemistry. In this report, a novel approach is proposed to assess hair histology and HSPG distribution changes in HFs at different phases of the hair growth cycle using infrared spectral imaging (IRSI). The distribution of HSPGs in HFs was probed by IRSI using the absorption region relevant to sulfation as a spectral marker. The findings were supported by Western immunoblotting and immunohistochemistry assays focusing on the glypican-1 expression and distribution in HFs. This study demonstrates the capacity of IRSI to identify the different HF tissue structures and to highlight protein, proteoglycan (PG), glycosaminoglycan (GAG), and sulfated GAG distribution in these structures. The comparison between anagen, catagen, and telogen phases shows the qualitative and/or quantitative evolution of GAGs as supported by Western immunoblotting. Thus, IRSI can simultaneously reveal the location of proteins, PGs, GAGs, and sulfated GAGs in HFs in a reagent- and label-free manner. From a dermatological point of view, IRSI shows its potential as a promising technique to study alopecia.
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spelling pubmed-79120312021-02-28 Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle Colin-Pierre, Charlie Untereiner, Valérie Sockalingum, Ganesh D. Berthélémy, Nicolas Danoux, Louis Bardey, Vincent Mine, Solène Jeanmaire, Christine Ramont, Laurent Brézillon, Stéphane Biomolecules Article The expression of glypicans in different hair follicle (HF) compartments and their potential roles during hair shaft growth are still poorly understood. Heparan sulfate proteoglycan (HSPG) distribution in HFs is classically investigated by conventional histology, biochemical analysis, and immunohistochemistry. In this report, a novel approach is proposed to assess hair histology and HSPG distribution changes in HFs at different phases of the hair growth cycle using infrared spectral imaging (IRSI). The distribution of HSPGs in HFs was probed by IRSI using the absorption region relevant to sulfation as a spectral marker. The findings were supported by Western immunoblotting and immunohistochemistry assays focusing on the glypican-1 expression and distribution in HFs. This study demonstrates the capacity of IRSI to identify the different HF tissue structures and to highlight protein, proteoglycan (PG), glycosaminoglycan (GAG), and sulfated GAG distribution in these structures. The comparison between anagen, catagen, and telogen phases shows the qualitative and/or quantitative evolution of GAGs as supported by Western immunoblotting. Thus, IRSI can simultaneously reveal the location of proteins, PGs, GAGs, and sulfated GAGs in HFs in a reagent- and label-free manner. From a dermatological point of view, IRSI shows its potential as a promising technique to study alopecia. MDPI 2021-01-30 /pmc/articles/PMC7912031/ /pubmed/33573119 http://dx.doi.org/10.3390/biom11020192 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Colin-Pierre, Charlie
Untereiner, Valérie
Sockalingum, Ganesh D.
Berthélémy, Nicolas
Danoux, Louis
Bardey, Vincent
Mine, Solène
Jeanmaire, Christine
Ramont, Laurent
Brézillon, Stéphane
Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle
title Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle
title_full Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle
title_fullStr Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle
title_full_unstemmed Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle
title_short Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle
title_sort hair histology and glycosaminoglycans distribution probed by infrared spectral imaging: focus on heparan sulfate proteoglycan and glypican-1 during hair growth cycle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912031/
https://www.ncbi.nlm.nih.gov/pubmed/33573119
http://dx.doi.org/10.3390/biom11020192
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