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Feasibility of Application of the Newly Developed Nano-Biomaterial, β-TCP/PDLLA, in Maxillofacial Reconstructive Surgery: A Pilot Rat Study

This study was performed to examine the applicability of the newly developed nano-biocomposite, β-tricalcium phosphate (β-TCP)/u-HA/poly-d/l-lactide (PDLLA), to bone defects in the oral and maxillofacial area. This novel nano-biocomposite showed several advantages, including biocompatibility, biodeg...

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Autores principales: Toda, Erina, Bai, Yunpeng, Sha, Jingjing, Dong, Quang Ngoc, Ngo, Huy Xuan, Suyama, Takashi, Miyamoto, Kenichi, Matsuzaki, Yumi, Kanno, Takahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912080/
https://www.ncbi.nlm.nih.gov/pubmed/33503931
http://dx.doi.org/10.3390/nano11020303
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author Toda, Erina
Bai, Yunpeng
Sha, Jingjing
Dong, Quang Ngoc
Ngo, Huy Xuan
Suyama, Takashi
Miyamoto, Kenichi
Matsuzaki, Yumi
Kanno, Takahiro
author_facet Toda, Erina
Bai, Yunpeng
Sha, Jingjing
Dong, Quang Ngoc
Ngo, Huy Xuan
Suyama, Takashi
Miyamoto, Kenichi
Matsuzaki, Yumi
Kanno, Takahiro
author_sort Toda, Erina
collection PubMed
description This study was performed to examine the applicability of the newly developed nano-biocomposite, β-tricalcium phosphate (β-TCP)/u-HA/poly-d/l-lactide (PDLLA), to bone defects in the oral and maxillofacial area. This novel nano-biocomposite showed several advantages, including biocompatibility, biodegradability, and osteoconductivity. In addition, its optimal plasticity also allowed its utilization in irregular critical bone defect reconstructive surgery. Here, three different nano-biomaterials, i.e., β-TCP/PDLLA, β-TCP, and PDLLA, were implanted into critical bone defects in the right lateral mandible of 10-week-old Sprague–Dawley (SD) rats as bone graft substitutes. Micro-computed tomography (Micro-CT) and immunohistochemical staining for the osteogenesis biomarkers, Runx2, osteocalcin, and the leptin receptor, were performed to investigate and compare bone regeneration between the groups. Although the micro-CT results showed the highest bone mineral density (BMD) and bone volume to total volume (BV/TV) with β-TCP, immunohistochemical analysis indicated better osteogenesis-promoting ability of β-TCP/PDLLA, especially at an early stage of the bone healing process. These results confirmed that the novel nano-biocomposite, β-TCP/PDLLA, which has excellent biocompatibility, bioresorbability and bioactive/osteoconductivity, has the potential to become a next-generation biomaterial for use as a bone graft substitute in maxillofacial reconstructive surgery.
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spelling pubmed-79120802021-02-28 Feasibility of Application of the Newly Developed Nano-Biomaterial, β-TCP/PDLLA, in Maxillofacial Reconstructive Surgery: A Pilot Rat Study Toda, Erina Bai, Yunpeng Sha, Jingjing Dong, Quang Ngoc Ngo, Huy Xuan Suyama, Takashi Miyamoto, Kenichi Matsuzaki, Yumi Kanno, Takahiro Nanomaterials (Basel) Article This study was performed to examine the applicability of the newly developed nano-biocomposite, β-tricalcium phosphate (β-TCP)/u-HA/poly-d/l-lactide (PDLLA), to bone defects in the oral and maxillofacial area. This novel nano-biocomposite showed several advantages, including biocompatibility, biodegradability, and osteoconductivity. In addition, its optimal plasticity also allowed its utilization in irregular critical bone defect reconstructive surgery. Here, three different nano-biomaterials, i.e., β-TCP/PDLLA, β-TCP, and PDLLA, were implanted into critical bone defects in the right lateral mandible of 10-week-old Sprague–Dawley (SD) rats as bone graft substitutes. Micro-computed tomography (Micro-CT) and immunohistochemical staining for the osteogenesis biomarkers, Runx2, osteocalcin, and the leptin receptor, were performed to investigate and compare bone regeneration between the groups. Although the micro-CT results showed the highest bone mineral density (BMD) and bone volume to total volume (BV/TV) with β-TCP, immunohistochemical analysis indicated better osteogenesis-promoting ability of β-TCP/PDLLA, especially at an early stage of the bone healing process. These results confirmed that the novel nano-biocomposite, β-TCP/PDLLA, which has excellent biocompatibility, bioresorbability and bioactive/osteoconductivity, has the potential to become a next-generation biomaterial for use as a bone graft substitute in maxillofacial reconstructive surgery. MDPI 2021-01-25 /pmc/articles/PMC7912080/ /pubmed/33503931 http://dx.doi.org/10.3390/nano11020303 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Toda, Erina
Bai, Yunpeng
Sha, Jingjing
Dong, Quang Ngoc
Ngo, Huy Xuan
Suyama, Takashi
Miyamoto, Kenichi
Matsuzaki, Yumi
Kanno, Takahiro
Feasibility of Application of the Newly Developed Nano-Biomaterial, β-TCP/PDLLA, in Maxillofacial Reconstructive Surgery: A Pilot Rat Study
title Feasibility of Application of the Newly Developed Nano-Biomaterial, β-TCP/PDLLA, in Maxillofacial Reconstructive Surgery: A Pilot Rat Study
title_full Feasibility of Application of the Newly Developed Nano-Biomaterial, β-TCP/PDLLA, in Maxillofacial Reconstructive Surgery: A Pilot Rat Study
title_fullStr Feasibility of Application of the Newly Developed Nano-Biomaterial, β-TCP/PDLLA, in Maxillofacial Reconstructive Surgery: A Pilot Rat Study
title_full_unstemmed Feasibility of Application of the Newly Developed Nano-Biomaterial, β-TCP/PDLLA, in Maxillofacial Reconstructive Surgery: A Pilot Rat Study
title_short Feasibility of Application of the Newly Developed Nano-Biomaterial, β-TCP/PDLLA, in Maxillofacial Reconstructive Surgery: A Pilot Rat Study
title_sort feasibility of application of the newly developed nano-biomaterial, β-tcp/pdlla, in maxillofacial reconstructive surgery: a pilot rat study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912080/
https://www.ncbi.nlm.nih.gov/pubmed/33503931
http://dx.doi.org/10.3390/nano11020303
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