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Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro

It is a widely known that heat stress induces a reduction in milk production in cows and impairs their overall health. Studies have shown that taurine protects tissues and organs under heat stress. However, there have yet to be studies showing the functions of taurine in mammary alveolar cells-large...

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Autores principales: Bai, Hui, Li, Tingting, Yu, Yan, Zhou, Ningcong, Kou, Huijuan, Guo, Yingying, Yang, Liang, Yan, Peishi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912084/
https://www.ncbi.nlm.nih.gov/pubmed/33525569
http://dx.doi.org/10.3390/cells10020258
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author Bai, Hui
Li, Tingting
Yu, Yan
Zhou, Ningcong
Kou, Huijuan
Guo, Yingying
Yang, Liang
Yan, Peishi
author_facet Bai, Hui
Li, Tingting
Yu, Yan
Zhou, Ningcong
Kou, Huijuan
Guo, Yingying
Yang, Liang
Yan, Peishi
author_sort Bai, Hui
collection PubMed
description It is a widely known that heat stress induces a reduction in milk production in cows and impairs their overall health. Studies have shown that taurine protects tissues and organs under heat stress. However, there have yet to be studies showing the functions of taurine in mammary alveolar cells-large T antigen (MAC-T) (a bovine mammary epithelial cell line) cells under heat shock. Therefore, different concentrations of taurine (10 mM, 50 mM, and 100 mM) were tested to determine the effects on heat-induced MAC-T cells. The results showed that taurine protected the cells against heat-induced damage as shown by morphological observations in conjunction with suppressed the translocation and expression of heat shock factor 1 (HSF1). Moreover, taurine not only reversed the decline in antioxidase (superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX)) activities but also attenuated the accumulation of malondialdehyde (MDA). Meanwhile, mitochondrial damage (morphology and complex I activity) resulting from heat exposure was mitigated. Taurine also alleviated the rates of cell apoptosis and markedly depressed the mRNA expressions of BCL2 associated X, apoptosis regulator (BAX) and caspase3. Furthermore, compared with the heat stress (HS) group, the protein levels of caspase3 and cleaved caspase3 were decreased in all taurine groups. In summary, taurine improves the antioxidant and anti-apoptosis ability of MAC-T cells thereby alleviates damage of cells due to heat insults.
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spelling pubmed-79120842021-02-28 Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro Bai, Hui Li, Tingting Yu, Yan Zhou, Ningcong Kou, Huijuan Guo, Yingying Yang, Liang Yan, Peishi Cells Article It is a widely known that heat stress induces a reduction in milk production in cows and impairs their overall health. Studies have shown that taurine protects tissues and organs under heat stress. However, there have yet to be studies showing the functions of taurine in mammary alveolar cells-large T antigen (MAC-T) (a bovine mammary epithelial cell line) cells under heat shock. Therefore, different concentrations of taurine (10 mM, 50 mM, and 100 mM) were tested to determine the effects on heat-induced MAC-T cells. The results showed that taurine protected the cells against heat-induced damage as shown by morphological observations in conjunction with suppressed the translocation and expression of heat shock factor 1 (HSF1). Moreover, taurine not only reversed the decline in antioxidase (superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX)) activities but also attenuated the accumulation of malondialdehyde (MDA). Meanwhile, mitochondrial damage (morphology and complex I activity) resulting from heat exposure was mitigated. Taurine also alleviated the rates of cell apoptosis and markedly depressed the mRNA expressions of BCL2 associated X, apoptosis regulator (BAX) and caspase3. Furthermore, compared with the heat stress (HS) group, the protein levels of caspase3 and cleaved caspase3 were decreased in all taurine groups. In summary, taurine improves the antioxidant and anti-apoptosis ability of MAC-T cells thereby alleviates damage of cells due to heat insults. MDPI 2021-01-28 /pmc/articles/PMC7912084/ /pubmed/33525569 http://dx.doi.org/10.3390/cells10020258 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bai, Hui
Li, Tingting
Yu, Yan
Zhou, Ningcong
Kou, Huijuan
Guo, Yingying
Yang, Liang
Yan, Peishi
Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro
title Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro
title_full Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro
title_fullStr Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro
title_full_unstemmed Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro
title_short Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro
title_sort cytoprotective effects of taurine on heat-induced bovine mammary epithelial cells in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912084/
https://www.ncbi.nlm.nih.gov/pubmed/33525569
http://dx.doi.org/10.3390/cells10020258
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