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Shedding Light on the African Enigma: In Vitro Testing of Homo sapiens-Helicobacter pylori Coevolution
The continuous characterization of genome-wide diversity in population and case–cohort samples, allied to the development of new algorithms, are shedding light on host ancestry impact and selection events on various infectious diseases. Especially interesting are the long-standing associations betwe...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912213/ https://www.ncbi.nlm.nih.gov/pubmed/33503840 http://dx.doi.org/10.3390/microorganisms9020240 |
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author | Cavadas, Bruno Leite, Marina Pedro, Nicole Magalhães, Ana C. Melo, Joana Correia, Marcelo Máximo, Valdemar Camacho, Rui Fonseca, Nuno A. Figueiredo, Ceu Pereira, Luísa |
author_facet | Cavadas, Bruno Leite, Marina Pedro, Nicole Magalhães, Ana C. Melo, Joana Correia, Marcelo Máximo, Valdemar Camacho, Rui Fonseca, Nuno A. Figueiredo, Ceu Pereira, Luísa |
author_sort | Cavadas, Bruno |
collection | PubMed |
description | The continuous characterization of genome-wide diversity in population and case–cohort samples, allied to the development of new algorithms, are shedding light on host ancestry impact and selection events on various infectious diseases. Especially interesting are the long-standing associations between humans and certain bacteria, such as the case of Helicobacter pylori, which could have been strong drivers of adaptation leading to coevolution. Some evidence on admixed gastric cancer cohorts have been suggested as supporting Homo-Helicobacter coevolution, but reliable experimental data that control both the bacterium and the host ancestries are lacking. Here, we conducted the first in vitro coinfection assays with dual human- and bacterium-matched and -mismatched ancestries, in African and European backgrounds, to evaluate the genome wide gene expression host response to H. pylori. Our results showed that: (1) the host response to H. pylori infection was greatly shaped by the human ancestry, with variability on innate immune system and metabolism; (2) African human ancestry showed signs of coevolution with H. pylori while European ancestry appeared to be maladapted; and (3) mismatched ancestry did not seem to be an important differentiator of gene expression at the initial stages of infection as assayed here. |
format | Online Article Text |
id | pubmed-7912213 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79122132021-02-28 Shedding Light on the African Enigma: In Vitro Testing of Homo sapiens-Helicobacter pylori Coevolution Cavadas, Bruno Leite, Marina Pedro, Nicole Magalhães, Ana C. Melo, Joana Correia, Marcelo Máximo, Valdemar Camacho, Rui Fonseca, Nuno A. Figueiredo, Ceu Pereira, Luísa Microorganisms Article The continuous characterization of genome-wide diversity in population and case–cohort samples, allied to the development of new algorithms, are shedding light on host ancestry impact and selection events on various infectious diseases. Especially interesting are the long-standing associations between humans and certain bacteria, such as the case of Helicobacter pylori, which could have been strong drivers of adaptation leading to coevolution. Some evidence on admixed gastric cancer cohorts have been suggested as supporting Homo-Helicobacter coevolution, but reliable experimental data that control both the bacterium and the host ancestries are lacking. Here, we conducted the first in vitro coinfection assays with dual human- and bacterium-matched and -mismatched ancestries, in African and European backgrounds, to evaluate the genome wide gene expression host response to H. pylori. Our results showed that: (1) the host response to H. pylori infection was greatly shaped by the human ancestry, with variability on innate immune system and metabolism; (2) African human ancestry showed signs of coevolution with H. pylori while European ancestry appeared to be maladapted; and (3) mismatched ancestry did not seem to be an important differentiator of gene expression at the initial stages of infection as assayed here. MDPI 2021-01-25 /pmc/articles/PMC7912213/ /pubmed/33503840 http://dx.doi.org/10.3390/microorganisms9020240 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cavadas, Bruno Leite, Marina Pedro, Nicole Magalhães, Ana C. Melo, Joana Correia, Marcelo Máximo, Valdemar Camacho, Rui Fonseca, Nuno A. Figueiredo, Ceu Pereira, Luísa Shedding Light on the African Enigma: In Vitro Testing of Homo sapiens-Helicobacter pylori Coevolution |
title | Shedding Light on the African Enigma: In Vitro Testing of Homo sapiens-Helicobacter pylori Coevolution |
title_full | Shedding Light on the African Enigma: In Vitro Testing of Homo sapiens-Helicobacter pylori Coevolution |
title_fullStr | Shedding Light on the African Enigma: In Vitro Testing of Homo sapiens-Helicobacter pylori Coevolution |
title_full_unstemmed | Shedding Light on the African Enigma: In Vitro Testing of Homo sapiens-Helicobacter pylori Coevolution |
title_short | Shedding Light on the African Enigma: In Vitro Testing of Homo sapiens-Helicobacter pylori Coevolution |
title_sort | shedding light on the african enigma: in vitro testing of homo sapiens-helicobacter pylori coevolution |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912213/ https://www.ncbi.nlm.nih.gov/pubmed/33503840 http://dx.doi.org/10.3390/microorganisms9020240 |
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