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Shedding Light on the African Enigma: In Vitro Testing of Homo sapiens-Helicobacter pylori Coevolution

The continuous characterization of genome-wide diversity in population and case–cohort samples, allied to the development of new algorithms, are shedding light on host ancestry impact and selection events on various infectious diseases. Especially interesting are the long-standing associations betwe...

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Autores principales: Cavadas, Bruno, Leite, Marina, Pedro, Nicole, Magalhães, Ana C., Melo, Joana, Correia, Marcelo, Máximo, Valdemar, Camacho, Rui, Fonseca, Nuno A., Figueiredo, Ceu, Pereira, Luísa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912213/
https://www.ncbi.nlm.nih.gov/pubmed/33503840
http://dx.doi.org/10.3390/microorganisms9020240
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author Cavadas, Bruno
Leite, Marina
Pedro, Nicole
Magalhães, Ana C.
Melo, Joana
Correia, Marcelo
Máximo, Valdemar
Camacho, Rui
Fonseca, Nuno A.
Figueiredo, Ceu
Pereira, Luísa
author_facet Cavadas, Bruno
Leite, Marina
Pedro, Nicole
Magalhães, Ana C.
Melo, Joana
Correia, Marcelo
Máximo, Valdemar
Camacho, Rui
Fonseca, Nuno A.
Figueiredo, Ceu
Pereira, Luísa
author_sort Cavadas, Bruno
collection PubMed
description The continuous characterization of genome-wide diversity in population and case–cohort samples, allied to the development of new algorithms, are shedding light on host ancestry impact and selection events on various infectious diseases. Especially interesting are the long-standing associations between humans and certain bacteria, such as the case of Helicobacter pylori, which could have been strong drivers of adaptation leading to coevolution. Some evidence on admixed gastric cancer cohorts have been suggested as supporting Homo-Helicobacter coevolution, but reliable experimental data that control both the bacterium and the host ancestries are lacking. Here, we conducted the first in vitro coinfection assays with dual human- and bacterium-matched and -mismatched ancestries, in African and European backgrounds, to evaluate the genome wide gene expression host response to H. pylori. Our results showed that: (1) the host response to H. pylori infection was greatly shaped by the human ancestry, with variability on innate immune system and metabolism; (2) African human ancestry showed signs of coevolution with H. pylori while European ancestry appeared to be maladapted; and (3) mismatched ancestry did not seem to be an important differentiator of gene expression at the initial stages of infection as assayed here.
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spelling pubmed-79122132021-02-28 Shedding Light on the African Enigma: In Vitro Testing of Homo sapiens-Helicobacter pylori Coevolution Cavadas, Bruno Leite, Marina Pedro, Nicole Magalhães, Ana C. Melo, Joana Correia, Marcelo Máximo, Valdemar Camacho, Rui Fonseca, Nuno A. Figueiredo, Ceu Pereira, Luísa Microorganisms Article The continuous characterization of genome-wide diversity in population and case–cohort samples, allied to the development of new algorithms, are shedding light on host ancestry impact and selection events on various infectious diseases. Especially interesting are the long-standing associations between humans and certain bacteria, such as the case of Helicobacter pylori, which could have been strong drivers of adaptation leading to coevolution. Some evidence on admixed gastric cancer cohorts have been suggested as supporting Homo-Helicobacter coevolution, but reliable experimental data that control both the bacterium and the host ancestries are lacking. Here, we conducted the first in vitro coinfection assays with dual human- and bacterium-matched and -mismatched ancestries, in African and European backgrounds, to evaluate the genome wide gene expression host response to H. pylori. Our results showed that: (1) the host response to H. pylori infection was greatly shaped by the human ancestry, with variability on innate immune system and metabolism; (2) African human ancestry showed signs of coevolution with H. pylori while European ancestry appeared to be maladapted; and (3) mismatched ancestry did not seem to be an important differentiator of gene expression at the initial stages of infection as assayed here. MDPI 2021-01-25 /pmc/articles/PMC7912213/ /pubmed/33503840 http://dx.doi.org/10.3390/microorganisms9020240 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cavadas, Bruno
Leite, Marina
Pedro, Nicole
Magalhães, Ana C.
Melo, Joana
Correia, Marcelo
Máximo, Valdemar
Camacho, Rui
Fonseca, Nuno A.
Figueiredo, Ceu
Pereira, Luísa
Shedding Light on the African Enigma: In Vitro Testing of Homo sapiens-Helicobacter pylori Coevolution
title Shedding Light on the African Enigma: In Vitro Testing of Homo sapiens-Helicobacter pylori Coevolution
title_full Shedding Light on the African Enigma: In Vitro Testing of Homo sapiens-Helicobacter pylori Coevolution
title_fullStr Shedding Light on the African Enigma: In Vitro Testing of Homo sapiens-Helicobacter pylori Coevolution
title_full_unstemmed Shedding Light on the African Enigma: In Vitro Testing of Homo sapiens-Helicobacter pylori Coevolution
title_short Shedding Light on the African Enigma: In Vitro Testing of Homo sapiens-Helicobacter pylori Coevolution
title_sort shedding light on the african enigma: in vitro testing of homo sapiens-helicobacter pylori coevolution
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912213/
https://www.ncbi.nlm.nih.gov/pubmed/33503840
http://dx.doi.org/10.3390/microorganisms9020240
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