Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA

The core proteins (HBc) of the hepatitis B virus (HBV) genotypes A, B, C, D, E, F, and G were cloned and expressed in Escherichia coli (E. coli), and HBc-formed virus-like particles (VLPs) were purified with ammonium sulfate precipitation, gel filtration, and ion exchange chromatography (IEX). The b...

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Autores principales: Petrovskis, Ivars, Lieknina, Ilva, Dislers, Andris, Jansons, Juris, Bogans, Janis, Akopjana, Inara, Zakova, Jelena, Sominskaya, Irina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912224/
https://www.ncbi.nlm.nih.gov/pubmed/33573151
http://dx.doi.org/10.3390/microorganisms9020283
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author Petrovskis, Ivars
Lieknina, Ilva
Dislers, Andris
Jansons, Juris
Bogans, Janis
Akopjana, Inara
Zakova, Jelena
Sominskaya, Irina
author_facet Petrovskis, Ivars
Lieknina, Ilva
Dislers, Andris
Jansons, Juris
Bogans, Janis
Akopjana, Inara
Zakova, Jelena
Sominskaya, Irina
author_sort Petrovskis, Ivars
collection PubMed
description The core proteins (HBc) of the hepatitis B virus (HBV) genotypes A, B, C, D, E, F, and G were cloned and expressed in Escherichia coli (E. coli), and HBc-formed virus-like particles (VLPs) were purified with ammonium sulfate precipitation, gel filtration, and ion exchange chromatography (IEX). The best VLP yield was found for the HBc of the HBV genotypes D and G. For the HBc of the HBV genotypes D, F, and G, the possibility of dissociation and reassociation maintaining the native HBc structure was demonstrated. Single-stranded (ss) and double-stranded (ds) ribonucleic acid (RNA) was successfully packed into HBc VLPs for the HBV genotypes D and G.
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spelling pubmed-79122242021-02-28 Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA Petrovskis, Ivars Lieknina, Ilva Dislers, Andris Jansons, Juris Bogans, Janis Akopjana, Inara Zakova, Jelena Sominskaya, Irina Microorganisms Article The core proteins (HBc) of the hepatitis B virus (HBV) genotypes A, B, C, D, E, F, and G were cloned and expressed in Escherichia coli (E. coli), and HBc-formed virus-like particles (VLPs) were purified with ammonium sulfate precipitation, gel filtration, and ion exchange chromatography (IEX). The best VLP yield was found for the HBc of the HBV genotypes D and G. For the HBc of the HBV genotypes D, F, and G, the possibility of dissociation and reassociation maintaining the native HBc structure was demonstrated. Single-stranded (ss) and double-stranded (ds) ribonucleic acid (RNA) was successfully packed into HBc VLPs for the HBV genotypes D and G. MDPI 2021-01-30 /pmc/articles/PMC7912224/ /pubmed/33573151 http://dx.doi.org/10.3390/microorganisms9020283 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Petrovskis, Ivars
Lieknina, Ilva
Dislers, Andris
Jansons, Juris
Bogans, Janis
Akopjana, Inara
Zakova, Jelena
Sominskaya, Irina
Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA
title Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA
title_full Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA
title_fullStr Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA
title_full_unstemmed Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA
title_short Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA
title_sort production of the hbc protein from different hbv genotypes in e. coli. use of reassociated hbc vlps for packaging of ss- and dsrna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912224/
https://www.ncbi.nlm.nih.gov/pubmed/33573151
http://dx.doi.org/10.3390/microorganisms9020283
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