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Involvement of Polyamine Metabolism in the Response of Medicago truncatula Genotypes to Salt Stress

Salinity constitutes one of the most important causes leading to severe reduction in plant yield. Several reports correlate the accumulation of polyamines in plants with tolerance to abiotic stress cues. The present study examined three Medicago truncatula genotypes with differing sensitivities to s...

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Autores principales: Antoniou, Chrystalla, Zarza, Xavier, Gohari, Gholamreza, Panahirad, Sima, Filippou, Panagiota, Tiburcio, Antonio F., Fotopoulos, Vasileios
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912313/
https://www.ncbi.nlm.nih.gov/pubmed/33573207
http://dx.doi.org/10.3390/plants10020269
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author Antoniou, Chrystalla
Zarza, Xavier
Gohari, Gholamreza
Panahirad, Sima
Filippou, Panagiota
Tiburcio, Antonio F.
Fotopoulos, Vasileios
author_facet Antoniou, Chrystalla
Zarza, Xavier
Gohari, Gholamreza
Panahirad, Sima
Filippou, Panagiota
Tiburcio, Antonio F.
Fotopoulos, Vasileios
author_sort Antoniou, Chrystalla
collection PubMed
description Salinity constitutes one of the most important causes leading to severe reduction in plant yield. Several reports correlate the accumulation of polyamines in plants with tolerance to abiotic stress cues. The present study examined three Medicago truncatula genotypes with differing sensitivities to salinity (TN1.11, tolerant; Jemalong A17, moderately sensitive; TN6.18, sensitive), with the aim of examining the genotype-specific involvement of the polyamine metabolic pathway in plant response to salinity. The study was carried out with leaves harvested 48 h after watering plants with 200 mM NaCl. A comprehensive profile of free polyamines was determined using high performance liquid chromatography. All genotypes showed spermidine and spermine as the most abundant polyamines under control conditions. In salinity conditions, spermine levels increased at the expense of putrescine and spermidine, indicating a drift of polyamine metabolism towards the synthesis of increasing polycationic forms as a stress response. The increasing balance between high and low polycationic forms was clearly diminished in the salt-sensitive genotype TN6.18, showing a clear correlation with its sensitive phenotype. The polyamine metabolic profile was then supported by molecular evidence through the examination of polyamine metabolism transcript levels by RT-qPCR. General suppression of genes that are involved upstream in the PA biosynthetic pathway was determined. Contrarily, an induction in the expression of genes involved in the biosynthesis of spermine and spermidine was observed, in agreement with the metabolic analysis. A significant induction in diamino oxidase expression, involved in the catabolism of putrescine, was specifically found in the sensitive genotype ΤΝ6.18, indicating a distinct metabolic response to stress. Present findings highlight the involvement of polyamines in the defense response of Medicago genotypes showing sensitivity to salt stress.
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spelling pubmed-79123132021-02-28 Involvement of Polyamine Metabolism in the Response of Medicago truncatula Genotypes to Salt Stress Antoniou, Chrystalla Zarza, Xavier Gohari, Gholamreza Panahirad, Sima Filippou, Panagiota Tiburcio, Antonio F. Fotopoulos, Vasileios Plants (Basel) Communication Salinity constitutes one of the most important causes leading to severe reduction in plant yield. Several reports correlate the accumulation of polyamines in plants with tolerance to abiotic stress cues. The present study examined three Medicago truncatula genotypes with differing sensitivities to salinity (TN1.11, tolerant; Jemalong A17, moderately sensitive; TN6.18, sensitive), with the aim of examining the genotype-specific involvement of the polyamine metabolic pathway in plant response to salinity. The study was carried out with leaves harvested 48 h after watering plants with 200 mM NaCl. A comprehensive profile of free polyamines was determined using high performance liquid chromatography. All genotypes showed spermidine and spermine as the most abundant polyamines under control conditions. In salinity conditions, spermine levels increased at the expense of putrescine and spermidine, indicating a drift of polyamine metabolism towards the synthesis of increasing polycationic forms as a stress response. The increasing balance between high and low polycationic forms was clearly diminished in the salt-sensitive genotype TN6.18, showing a clear correlation with its sensitive phenotype. The polyamine metabolic profile was then supported by molecular evidence through the examination of polyamine metabolism transcript levels by RT-qPCR. General suppression of genes that are involved upstream in the PA biosynthetic pathway was determined. Contrarily, an induction in the expression of genes involved in the biosynthesis of spermine and spermidine was observed, in agreement with the metabolic analysis. A significant induction in diamino oxidase expression, involved in the catabolism of putrescine, was specifically found in the sensitive genotype ΤΝ6.18, indicating a distinct metabolic response to stress. Present findings highlight the involvement of polyamines in the defense response of Medicago genotypes showing sensitivity to salt stress. MDPI 2021-01-30 /pmc/articles/PMC7912313/ /pubmed/33573207 http://dx.doi.org/10.3390/plants10020269 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Antoniou, Chrystalla
Zarza, Xavier
Gohari, Gholamreza
Panahirad, Sima
Filippou, Panagiota
Tiburcio, Antonio F.
Fotopoulos, Vasileios
Involvement of Polyamine Metabolism in the Response of Medicago truncatula Genotypes to Salt Stress
title Involvement of Polyamine Metabolism in the Response of Medicago truncatula Genotypes to Salt Stress
title_full Involvement of Polyamine Metabolism in the Response of Medicago truncatula Genotypes to Salt Stress
title_fullStr Involvement of Polyamine Metabolism in the Response of Medicago truncatula Genotypes to Salt Stress
title_full_unstemmed Involvement of Polyamine Metabolism in the Response of Medicago truncatula Genotypes to Salt Stress
title_short Involvement of Polyamine Metabolism in the Response of Medicago truncatula Genotypes to Salt Stress
title_sort involvement of polyamine metabolism in the response of medicago truncatula genotypes to salt stress
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912313/
https://www.ncbi.nlm.nih.gov/pubmed/33573207
http://dx.doi.org/10.3390/plants10020269
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