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Diversity and plant growth-promoting potential of (un)culturable bacteria in the Hedera helix phylloplane
BACKGROUND: A diverse community of microbes naturally exists on the phylloplane, the surface of leaves. It is one of the most prevalent microbial habitats on earth and bacteria are the most abundant members, living in communities that are highly dynamic. Today, one of the key challenges for microbio...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912551/ https://www.ncbi.nlm.nih.gov/pubmed/33639859 http://dx.doi.org/10.1186/s12866-021-02119-z |
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author | Stevens, Vincent Thijs, Sofie Vangronsveld, Jaco |
author_facet | Stevens, Vincent Thijs, Sofie Vangronsveld, Jaco |
author_sort | Stevens, Vincent |
collection | PubMed |
description | BACKGROUND: A diverse community of microbes naturally exists on the phylloplane, the surface of leaves. It is one of the most prevalent microbial habitats on earth and bacteria are the most abundant members, living in communities that are highly dynamic. Today, one of the key challenges for microbiologists is to develop strategies to culture the vast diversity of microorganisms that have been detected in metagenomic surveys. RESULTS: We isolated bacteria from the phylloplane of Hedera helix (common ivy), a widespread evergreen, using five growth media: Luria–Bertani (LB), LB01, yeast extract–mannitol (YMA), yeast extract–flour (YFlour), and YEx. We also included a comparison with the uncultured phylloplane, which we showed to be dominated by Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes. Inter-sample (beta) diversity shifted from LB and LB01 containing the highest amount of resources to YEx, YMA, and YFlour which are more selective. All growth media equally favoured Actinobacteria and Gammaproteobacteria, whereas Bacteroidetes could only be found on LB01, YEx, and YMA. LB and LB01 favoured Firmicutes and YFlour was most selective for Betaproteobacteria. At the genus level, LB favoured the growth of Bacillus and Stenotrophomonas, while YFlour was most selective for Burkholderia and Curtobacterium. The in vitro plant growth promotion (PGP) profile of 200 isolates obtained in this study indicates that previously uncultured bacteria from the phylloplane may have potential applications in phytoremediation and other plant-based biotechnologies. CONCLUSIONS: This study gives first insights into the total bacterial community of the H. helix phylloplane, including an evaluation of its culturability using five different growth media. We further provide a collection of 200 bacterial isolates underrepresented in current databases, including the characterization of PGP profiles. Here we highlight the potential of simple strategies to obtain higher microbial diversity from environmental samples and the use of high-throughput sequencing to guide isolate selection from a variety of growth media. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-021-02119-z. |
format | Online Article Text |
id | pubmed-7912551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-79125512021-03-02 Diversity and plant growth-promoting potential of (un)culturable bacteria in the Hedera helix phylloplane Stevens, Vincent Thijs, Sofie Vangronsveld, Jaco BMC Microbiol Research Article BACKGROUND: A diverse community of microbes naturally exists on the phylloplane, the surface of leaves. It is one of the most prevalent microbial habitats on earth and bacteria are the most abundant members, living in communities that are highly dynamic. Today, one of the key challenges for microbiologists is to develop strategies to culture the vast diversity of microorganisms that have been detected in metagenomic surveys. RESULTS: We isolated bacteria from the phylloplane of Hedera helix (common ivy), a widespread evergreen, using five growth media: Luria–Bertani (LB), LB01, yeast extract–mannitol (YMA), yeast extract–flour (YFlour), and YEx. We also included a comparison with the uncultured phylloplane, which we showed to be dominated by Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes. Inter-sample (beta) diversity shifted from LB and LB01 containing the highest amount of resources to YEx, YMA, and YFlour which are more selective. All growth media equally favoured Actinobacteria and Gammaproteobacteria, whereas Bacteroidetes could only be found on LB01, YEx, and YMA. LB and LB01 favoured Firmicutes and YFlour was most selective for Betaproteobacteria. At the genus level, LB favoured the growth of Bacillus and Stenotrophomonas, while YFlour was most selective for Burkholderia and Curtobacterium. The in vitro plant growth promotion (PGP) profile of 200 isolates obtained in this study indicates that previously uncultured bacteria from the phylloplane may have potential applications in phytoremediation and other plant-based biotechnologies. CONCLUSIONS: This study gives first insights into the total bacterial community of the H. helix phylloplane, including an evaluation of its culturability using five different growth media. We further provide a collection of 200 bacterial isolates underrepresented in current databases, including the characterization of PGP profiles. Here we highlight the potential of simple strategies to obtain higher microbial diversity from environmental samples and the use of high-throughput sequencing to guide isolate selection from a variety of growth media. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-021-02119-z. BioMed Central 2021-02-27 /pmc/articles/PMC7912551/ /pubmed/33639859 http://dx.doi.org/10.1186/s12866-021-02119-z Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Stevens, Vincent Thijs, Sofie Vangronsveld, Jaco Diversity and plant growth-promoting potential of (un)culturable bacteria in the Hedera helix phylloplane |
title | Diversity and plant growth-promoting potential of (un)culturable bacteria in the Hedera helix phylloplane |
title_full | Diversity and plant growth-promoting potential of (un)culturable bacteria in the Hedera helix phylloplane |
title_fullStr | Diversity and plant growth-promoting potential of (un)culturable bacteria in the Hedera helix phylloplane |
title_full_unstemmed | Diversity and plant growth-promoting potential of (un)culturable bacteria in the Hedera helix phylloplane |
title_short | Diversity and plant growth-promoting potential of (un)culturable bacteria in the Hedera helix phylloplane |
title_sort | diversity and plant growth-promoting potential of (un)culturable bacteria in the hedera helix phylloplane |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912551/ https://www.ncbi.nlm.nih.gov/pubmed/33639859 http://dx.doi.org/10.1186/s12866-021-02119-z |
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