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Genes and SNPs Involved with Scrotal and Umbilical Hernia in Pigs

Hernia is one of the most common defects in pigs. The most prevalent are the scrotal (SH), inguinal (IH) and umbilical (UH) hernias. We compared the inguinal ring transcriptome of normal and SH-affected pigs with the umbilical ring transcriptome of normal and UH-affected pigs to discover genes and p...

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Autores principales: Rodrigues, Ariene Fernanda Grando, Ibelli, Adriana Mércia Guaratini, Peixoto, Jane de Oliveira, Cantão, Maurício Egídio, de Oliveira, Haniel Cedraz, Savoldi, Igor Ricardo, Souza, Mayla Regina, Mores, Marcos Antônio Zanella, Carreño, Luis Orlando Duitama, Ledur, Mônica Corrêa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912685/
https://www.ncbi.nlm.nih.gov/pubmed/33513662
http://dx.doi.org/10.3390/genes12020166
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author Rodrigues, Ariene Fernanda Grando
Ibelli, Adriana Mércia Guaratini
Peixoto, Jane de Oliveira
Cantão, Maurício Egídio
de Oliveira, Haniel Cedraz
Savoldi, Igor Ricardo
Souza, Mayla Regina
Mores, Marcos Antônio Zanella
Carreño, Luis Orlando Duitama
Ledur, Mônica Corrêa
author_facet Rodrigues, Ariene Fernanda Grando
Ibelli, Adriana Mércia Guaratini
Peixoto, Jane de Oliveira
Cantão, Maurício Egídio
de Oliveira, Haniel Cedraz
Savoldi, Igor Ricardo
Souza, Mayla Regina
Mores, Marcos Antônio Zanella
Carreño, Luis Orlando Duitama
Ledur, Mônica Corrêa
author_sort Rodrigues, Ariene Fernanda Grando
collection PubMed
description Hernia is one of the most common defects in pigs. The most prevalent are the scrotal (SH), inguinal (IH) and umbilical (UH) hernias. We compared the inguinal ring transcriptome of normal and SH-affected pigs with the umbilical ring transcriptome of normal and UH-affected pigs to discover genes and pathways involved with the development of both types of hernia. A total of 13,307 transcripts was expressed in the inguinal and 13,302 in the umbilical ring tissues with 94.91% of them present in both tissues. From those, 35 genes were differentially expressed in both groups, participating in 108 biological processes. A total of 67 polymorphisms was identified in the inguinal ring and 76 in the umbilical ring tissue, of which 11 and 14 were novel, respectively. A single nucleotide polymorphism (SNP) with deleterious function was identified in the integrin α M (ITGAM) gene. The microtubule associated protein 1 light chain 3 γ (MAP1LC3C), vitrin (VIT), aggrecan (ACAN), alkaline ceramidase 2 (ACER2), potassium calcium-activated channel subfamily M α 1 (KCNMA1) and synaptopodin 2 (SYNPO2) genes are highlighted as candidates to trigger both types of hernia. We generated the first comparative study of the pig umbilical and inguinal ring transcriptomes, contributing to the understanding of the genetic mechanism involved with these two types of hernia in pigs and probably in other mammals.
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spelling pubmed-79126852021-02-28 Genes and SNPs Involved with Scrotal and Umbilical Hernia in Pigs Rodrigues, Ariene Fernanda Grando Ibelli, Adriana Mércia Guaratini Peixoto, Jane de Oliveira Cantão, Maurício Egídio de Oliveira, Haniel Cedraz Savoldi, Igor Ricardo Souza, Mayla Regina Mores, Marcos Antônio Zanella Carreño, Luis Orlando Duitama Ledur, Mônica Corrêa Genes (Basel) Article Hernia is one of the most common defects in pigs. The most prevalent are the scrotal (SH), inguinal (IH) and umbilical (UH) hernias. We compared the inguinal ring transcriptome of normal and SH-affected pigs with the umbilical ring transcriptome of normal and UH-affected pigs to discover genes and pathways involved with the development of both types of hernia. A total of 13,307 transcripts was expressed in the inguinal and 13,302 in the umbilical ring tissues with 94.91% of them present in both tissues. From those, 35 genes were differentially expressed in both groups, participating in 108 biological processes. A total of 67 polymorphisms was identified in the inguinal ring and 76 in the umbilical ring tissue, of which 11 and 14 were novel, respectively. A single nucleotide polymorphism (SNP) with deleterious function was identified in the integrin α M (ITGAM) gene. The microtubule associated protein 1 light chain 3 γ (MAP1LC3C), vitrin (VIT), aggrecan (ACAN), alkaline ceramidase 2 (ACER2), potassium calcium-activated channel subfamily M α 1 (KCNMA1) and synaptopodin 2 (SYNPO2) genes are highlighted as candidates to trigger both types of hernia. We generated the first comparative study of the pig umbilical and inguinal ring transcriptomes, contributing to the understanding of the genetic mechanism involved with these two types of hernia in pigs and probably in other mammals. MDPI 2021-01-27 /pmc/articles/PMC7912685/ /pubmed/33513662 http://dx.doi.org/10.3390/genes12020166 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rodrigues, Ariene Fernanda Grando
Ibelli, Adriana Mércia Guaratini
Peixoto, Jane de Oliveira
Cantão, Maurício Egídio
de Oliveira, Haniel Cedraz
Savoldi, Igor Ricardo
Souza, Mayla Regina
Mores, Marcos Antônio Zanella
Carreño, Luis Orlando Duitama
Ledur, Mônica Corrêa
Genes and SNPs Involved with Scrotal and Umbilical Hernia in Pigs
title Genes and SNPs Involved with Scrotal and Umbilical Hernia in Pigs
title_full Genes and SNPs Involved with Scrotal and Umbilical Hernia in Pigs
title_fullStr Genes and SNPs Involved with Scrotal and Umbilical Hernia in Pigs
title_full_unstemmed Genes and SNPs Involved with Scrotal and Umbilical Hernia in Pigs
title_short Genes and SNPs Involved with Scrotal and Umbilical Hernia in Pigs
title_sort genes and snps involved with scrotal and umbilical hernia in pigs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912685/
https://www.ncbi.nlm.nih.gov/pubmed/33513662
http://dx.doi.org/10.3390/genes12020166
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