Cargando…
Dynamics of a Dual SARS-CoV-2 Lineage Co-Infection on a Prolonged Viral Shedding COVID-19 Case: Insights into Clinical Severity and Disease Duration
A few molecularly proven severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cases of symptomatic reinfection are currently known worldwide, with a resolved first infection followed by a second infection after a 48 to 142-day intervening period. We report a multiple-component study of a cli...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912897/ https://www.ncbi.nlm.nih.gov/pubmed/33540596 http://dx.doi.org/10.3390/microorganisms9020300 |
_version_ | 1783656681417211904 |
---|---|
author | Pedro, Nicole Silva, Cláudio N. Magalhães, Ana C. Cavadas, Bruno Rocha, Ana M. Moreira, Ana C. Gomes, Maria Salomé Silva, Diogo Sobrinho-Simões, Joana Ramos, Angélica Cardoso, Maria J. Filipe, Rita Palma, Pedro Ceia, Filipa Silva, Susana Guimarães, João T. Sarmento, António Fernandes, Verónica Pereira, Luisa Tavares, Margarida |
author_facet | Pedro, Nicole Silva, Cláudio N. Magalhães, Ana C. Cavadas, Bruno Rocha, Ana M. Moreira, Ana C. Gomes, Maria Salomé Silva, Diogo Sobrinho-Simões, Joana Ramos, Angélica Cardoso, Maria J. Filipe, Rita Palma, Pedro Ceia, Filipa Silva, Susana Guimarães, João T. Sarmento, António Fernandes, Verónica Pereira, Luisa Tavares, Margarida |
author_sort | Pedro, Nicole |
collection | PubMed |
description | A few molecularly proven severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cases of symptomatic reinfection are currently known worldwide, with a resolved first infection followed by a second infection after a 48 to 142-day intervening period. We report a multiple-component study of a clinically severe and prolonged viral shedding coronavirus disease 2019 (COVID-19) case in a 17-year-old Portuguese female. She had two hospitalizations, a total of 19 RT-PCR tests, mostly positive, and criteria for releasing from home isolation at the end of 97 days. The viral genome was sequenced in seven serial samples and in the diagnostic sample from her infected mother. A human genome-wide array (>900 K) was screened on the seven samples, and in vitro culture was conducted on isolates from three late samples. The patient had co-infection by two SARS-CoV-2 lineages, which were affiliated in distinct clades and diverging by six variants. The 20A lineage was absolute at the diagnosis (shared with the patient’s mother), but nine days later, the 20B lineage had 3% frequency, and two months later, the 20B lineage had 100% frequency. The 900 K profiles confirmed the identity of the patient in the serial samples, and they allowed us to infer that she had polygenic risk scores for hospitalization and severe respiratory disease within the normal distributions for a Portuguese population cohort. The early-on dynamic co-infection may have contributed to the severity of COVID-19 in this otherwise healthy young patient, and to her prolonged SARS-CoV-2 shedding profile. |
format | Online Article Text |
id | pubmed-7912897 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79128972021-02-28 Dynamics of a Dual SARS-CoV-2 Lineage Co-Infection on a Prolonged Viral Shedding COVID-19 Case: Insights into Clinical Severity and Disease Duration Pedro, Nicole Silva, Cláudio N. Magalhães, Ana C. Cavadas, Bruno Rocha, Ana M. Moreira, Ana C. Gomes, Maria Salomé Silva, Diogo Sobrinho-Simões, Joana Ramos, Angélica Cardoso, Maria J. Filipe, Rita Palma, Pedro Ceia, Filipa Silva, Susana Guimarães, João T. Sarmento, António Fernandes, Verónica Pereira, Luisa Tavares, Margarida Microorganisms Article A few molecularly proven severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cases of symptomatic reinfection are currently known worldwide, with a resolved first infection followed by a second infection after a 48 to 142-day intervening period. We report a multiple-component study of a clinically severe and prolonged viral shedding coronavirus disease 2019 (COVID-19) case in a 17-year-old Portuguese female. She had two hospitalizations, a total of 19 RT-PCR tests, mostly positive, and criteria for releasing from home isolation at the end of 97 days. The viral genome was sequenced in seven serial samples and in the diagnostic sample from her infected mother. A human genome-wide array (>900 K) was screened on the seven samples, and in vitro culture was conducted on isolates from three late samples. The patient had co-infection by two SARS-CoV-2 lineages, which were affiliated in distinct clades and diverging by six variants. The 20A lineage was absolute at the diagnosis (shared with the patient’s mother), but nine days later, the 20B lineage had 3% frequency, and two months later, the 20B lineage had 100% frequency. The 900 K profiles confirmed the identity of the patient in the serial samples, and they allowed us to infer that she had polygenic risk scores for hospitalization and severe respiratory disease within the normal distributions for a Portuguese population cohort. The early-on dynamic co-infection may have contributed to the severity of COVID-19 in this otherwise healthy young patient, and to her prolonged SARS-CoV-2 shedding profile. MDPI 2021-02-02 /pmc/articles/PMC7912897/ /pubmed/33540596 http://dx.doi.org/10.3390/microorganisms9020300 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pedro, Nicole Silva, Cláudio N. Magalhães, Ana C. Cavadas, Bruno Rocha, Ana M. Moreira, Ana C. Gomes, Maria Salomé Silva, Diogo Sobrinho-Simões, Joana Ramos, Angélica Cardoso, Maria J. Filipe, Rita Palma, Pedro Ceia, Filipa Silva, Susana Guimarães, João T. Sarmento, António Fernandes, Verónica Pereira, Luisa Tavares, Margarida Dynamics of a Dual SARS-CoV-2 Lineage Co-Infection on a Prolonged Viral Shedding COVID-19 Case: Insights into Clinical Severity and Disease Duration |
title | Dynamics of a Dual SARS-CoV-2 Lineage Co-Infection on a Prolonged Viral Shedding COVID-19 Case: Insights into Clinical Severity and Disease Duration |
title_full | Dynamics of a Dual SARS-CoV-2 Lineage Co-Infection on a Prolonged Viral Shedding COVID-19 Case: Insights into Clinical Severity and Disease Duration |
title_fullStr | Dynamics of a Dual SARS-CoV-2 Lineage Co-Infection on a Prolonged Viral Shedding COVID-19 Case: Insights into Clinical Severity and Disease Duration |
title_full_unstemmed | Dynamics of a Dual SARS-CoV-2 Lineage Co-Infection on a Prolonged Viral Shedding COVID-19 Case: Insights into Clinical Severity and Disease Duration |
title_short | Dynamics of a Dual SARS-CoV-2 Lineage Co-Infection on a Prolonged Viral Shedding COVID-19 Case: Insights into Clinical Severity and Disease Duration |
title_sort | dynamics of a dual sars-cov-2 lineage co-infection on a prolonged viral shedding covid-19 case: insights into clinical severity and disease duration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912897/ https://www.ncbi.nlm.nih.gov/pubmed/33540596 http://dx.doi.org/10.3390/microorganisms9020300 |
work_keys_str_mv | AT pedronicole dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT silvaclaudion dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT magalhaesanac dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT cavadasbruno dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT rochaanam dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT moreiraanac dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT gomesmariasalome dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT silvadiogo dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT sobrinhosimoesjoana dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT ramosangelica dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT cardosomariaj dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT filiperita dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT palmapedro dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT ceiafilipa dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT silvasusana dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT guimaraesjoaot dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT sarmentoantonio dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT fernandesveronica dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT pereiraluisa dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration AT tavaresmargarida dynamicsofadualsarscov2lineagecoinfectiononaprolongedviralsheddingcovid19caseinsightsintoclinicalseverityanddiseaseduration |