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Influences of Molecular Weights on Physicochemical and Biological Properties of Collagen-Alginate Scaffolds

For tissue engineering applications, biodegradable scaffolds containing high molecular weights (MW) of collagen and sodium alginate have been developed and characterized. However, the properties of low MW collagen-based scaffolds have not been studied in previous research. This work examined the dis...

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Autores principales: Ho, Truc Cong, Park, Jin-Seok, Kim, Sung-Yeoul, Lee, Hoyeol, Lim, Ju-Sop, Kim, Shin-Jun, Choi, Mi-Hee, Nam, Seung Yun, Chun, Byung-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912951/
https://www.ncbi.nlm.nih.gov/pubmed/33540717
http://dx.doi.org/10.3390/md19020085
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author Ho, Truc Cong
Park, Jin-Seok
Kim, Sung-Yeoul
Lee, Hoyeol
Lim, Ju-Sop
Kim, Shin-Jun
Choi, Mi-Hee
Nam, Seung Yun
Chun, Byung-Soo
author_facet Ho, Truc Cong
Park, Jin-Seok
Kim, Sung-Yeoul
Lee, Hoyeol
Lim, Ju-Sop
Kim, Shin-Jun
Choi, Mi-Hee
Nam, Seung Yun
Chun, Byung-Soo
author_sort Ho, Truc Cong
collection PubMed
description For tissue engineering applications, biodegradable scaffolds containing high molecular weights (MW) of collagen and sodium alginate have been developed and characterized. However, the properties of low MW collagen-based scaffolds have not been studied in previous research. This work examined the distinctive properties of low MW collagen-based scaffolds with alginate unmodified and modified by subcritical water. Besides, we developed a facile method to cross-link water-soluble scaffolds using glutaraldehyde in an aqueous ethanol solution. The prepared cross-linked scaffolds showed good structural properties with high porosity (~93%) and high cross-linking degree (50–60%). Compared with collagen (6000 Da)-based scaffolds, collagen (25,000 Da)-based scaffolds exhibited higher stability against collagenase degradation and lower weight loss in phosphate buffer pH 7.4. Collagen (25,000 Da)-based scaffolds with modified alginate tended to improve antioxidant capacity compared with scaffolds containing unmodified alginate. Interestingly, in vitro coagulant activity assay demonstrated that collagen (25,000 Da)-based scaffolds with modified alginate (C25-A63 and C25-A21) significantly reduced the clotting time of human plasma compared with scaffolds consisting of unmodified alginate. Although some further investigations need to be done, collagen (25,000 Da)-based scaffolds with modified alginate should be considered as a potential candidate for tissue engineering applications.
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spelling pubmed-79129512021-02-28 Influences of Molecular Weights on Physicochemical and Biological Properties of Collagen-Alginate Scaffolds Ho, Truc Cong Park, Jin-Seok Kim, Sung-Yeoul Lee, Hoyeol Lim, Ju-Sop Kim, Shin-Jun Choi, Mi-Hee Nam, Seung Yun Chun, Byung-Soo Mar Drugs Article For tissue engineering applications, biodegradable scaffolds containing high molecular weights (MW) of collagen and sodium alginate have been developed and characterized. However, the properties of low MW collagen-based scaffolds have not been studied in previous research. This work examined the distinctive properties of low MW collagen-based scaffolds with alginate unmodified and modified by subcritical water. Besides, we developed a facile method to cross-link water-soluble scaffolds using glutaraldehyde in an aqueous ethanol solution. The prepared cross-linked scaffolds showed good structural properties with high porosity (~93%) and high cross-linking degree (50–60%). Compared with collagen (6000 Da)-based scaffolds, collagen (25,000 Da)-based scaffolds exhibited higher stability against collagenase degradation and lower weight loss in phosphate buffer pH 7.4. Collagen (25,000 Da)-based scaffolds with modified alginate tended to improve antioxidant capacity compared with scaffolds containing unmodified alginate. Interestingly, in vitro coagulant activity assay demonstrated that collagen (25,000 Da)-based scaffolds with modified alginate (C25-A63 and C25-A21) significantly reduced the clotting time of human plasma compared with scaffolds consisting of unmodified alginate. Although some further investigations need to be done, collagen (25,000 Da)-based scaffolds with modified alginate should be considered as a potential candidate for tissue engineering applications. MDPI 2021-02-02 /pmc/articles/PMC7912951/ /pubmed/33540717 http://dx.doi.org/10.3390/md19020085 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ho, Truc Cong
Park, Jin-Seok
Kim, Sung-Yeoul
Lee, Hoyeol
Lim, Ju-Sop
Kim, Shin-Jun
Choi, Mi-Hee
Nam, Seung Yun
Chun, Byung-Soo
Influences of Molecular Weights on Physicochemical and Biological Properties of Collagen-Alginate Scaffolds
title Influences of Molecular Weights on Physicochemical and Biological Properties of Collagen-Alginate Scaffolds
title_full Influences of Molecular Weights on Physicochemical and Biological Properties of Collagen-Alginate Scaffolds
title_fullStr Influences of Molecular Weights on Physicochemical and Biological Properties of Collagen-Alginate Scaffolds
title_full_unstemmed Influences of Molecular Weights on Physicochemical and Biological Properties of Collagen-Alginate Scaffolds
title_short Influences of Molecular Weights on Physicochemical and Biological Properties of Collagen-Alginate Scaffolds
title_sort influences of molecular weights on physicochemical and biological properties of collagen-alginate scaffolds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912951/
https://www.ncbi.nlm.nih.gov/pubmed/33540717
http://dx.doi.org/10.3390/md19020085
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