Cargando…

Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical Reconstruction

Methicillin-susceptible (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen commonly found in bone and joint infections, including septic arthritis. S. aureus virulence and the frailty of affected patients can cause several complications; a prompt and specific antibiotic trea...

Descripción completa

Detalles Bibliográficos
Autores principales: Cafiso, Viviana, Lo Verde, Flavia, Zega, Alessandra, Pigola, Giuseppe, Rostagno, Roberto, Borrè, Silvio, Stefani, Stefania
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913009/
https://www.ncbi.nlm.nih.gov/pubmed/33540689
http://dx.doi.org/10.3390/microorganisms9020305
_version_ 1783656706846228480
author Cafiso, Viviana
Lo Verde, Flavia
Zega, Alessandra
Pigola, Giuseppe
Rostagno, Roberto
Borrè, Silvio
Stefani, Stefania
author_facet Cafiso, Viviana
Lo Verde, Flavia
Zega, Alessandra
Pigola, Giuseppe
Rostagno, Roberto
Borrè, Silvio
Stefani, Stefania
author_sort Cafiso, Viviana
collection PubMed
description Methicillin-susceptible (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen commonly found in bone and joint infections, including septic arthritis. S. aureus virulence and the frailty of affected patients can cause several complications; a prompt and specific antibiotic treatment can positively affect the outcome of patients. We carried out an in-depth genomic characterization by Illumina whole genome sequencing and bioinformatics of two biofilm-producing M1 and M2 ST398 MSSA causing septic knee arthritis not-responding to antimicrobial therapy. The strains were characterized for antibiotic resistance, biofilm and adhesive properties as well as genomics, single nucleotide polymorphism phylogeny, resistomics and virulomics. Our results showed that M1 and M2 MSSA were ST398-t1451-agrI-Cap5, susceptible to cefoxitin and resistant to erythromycin and clindamycin, traits consistent with the lack of the SCCmec-locus and the presence of the sole blaZ and ermT. Furthermore, M1 and M2 were biofilm-producing and largely potentially adhesive strains, as indicated by the adhesion gene profile. Our data characterized a new human-adapted ST398 MSSA lineage, representing a “fusion” between the human-animal independent ST398 and the Livestock Associated (LA) ST398 lineages, forming biofilm and genomically predicted high adhesive, characterized by different genomic adaptation conferring a great ability to adhere to the host’s extracellular matrix causing septic knee arthritis.
format Online
Article
Text
id pubmed-7913009
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79130092021-02-28 Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical Reconstruction Cafiso, Viviana Lo Verde, Flavia Zega, Alessandra Pigola, Giuseppe Rostagno, Roberto Borrè, Silvio Stefani, Stefania Microorganisms Article Methicillin-susceptible (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen commonly found in bone and joint infections, including septic arthritis. S. aureus virulence and the frailty of affected patients can cause several complications; a prompt and specific antibiotic treatment can positively affect the outcome of patients. We carried out an in-depth genomic characterization by Illumina whole genome sequencing and bioinformatics of two biofilm-producing M1 and M2 ST398 MSSA causing septic knee arthritis not-responding to antimicrobial therapy. The strains were characterized for antibiotic resistance, biofilm and adhesive properties as well as genomics, single nucleotide polymorphism phylogeny, resistomics and virulomics. Our results showed that M1 and M2 MSSA were ST398-t1451-agrI-Cap5, susceptible to cefoxitin and resistant to erythromycin and clindamycin, traits consistent with the lack of the SCCmec-locus and the presence of the sole blaZ and ermT. Furthermore, M1 and M2 were biofilm-producing and largely potentially adhesive strains, as indicated by the adhesion gene profile. Our data characterized a new human-adapted ST398 MSSA lineage, representing a “fusion” between the human-animal independent ST398 and the Livestock Associated (LA) ST398 lineages, forming biofilm and genomically predicted high adhesive, characterized by different genomic adaptation conferring a great ability to adhere to the host’s extracellular matrix causing septic knee arthritis. MDPI 2021-02-02 /pmc/articles/PMC7913009/ /pubmed/33540689 http://dx.doi.org/10.3390/microorganisms9020305 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cafiso, Viviana
Lo Verde, Flavia
Zega, Alessandra
Pigola, Giuseppe
Rostagno, Roberto
Borrè, Silvio
Stefani, Stefania
Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical Reconstruction
title Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical Reconstruction
title_full Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical Reconstruction
title_fullStr Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical Reconstruction
title_full_unstemmed Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical Reconstruction
title_short Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical Reconstruction
title_sort genomic characterization of a new biofilm-forming and adhesive st398 human-adapted mssa lineage causing septic knee arthritis following surgical reconstruction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913009/
https://www.ncbi.nlm.nih.gov/pubmed/33540689
http://dx.doi.org/10.3390/microorganisms9020305
work_keys_str_mv AT cafisoviviana genomiccharacterizationofanewbiofilmformingandadhesivest398humanadaptedmssalineagecausingseptickneearthritisfollowingsurgicalreconstruction
AT loverdeflavia genomiccharacterizationofanewbiofilmformingandadhesivest398humanadaptedmssalineagecausingseptickneearthritisfollowingsurgicalreconstruction
AT zegaalessandra genomiccharacterizationofanewbiofilmformingandadhesivest398humanadaptedmssalineagecausingseptickneearthritisfollowingsurgicalreconstruction
AT pigolagiuseppe genomiccharacterizationofanewbiofilmformingandadhesivest398humanadaptedmssalineagecausingseptickneearthritisfollowingsurgicalreconstruction
AT rostagnoroberto genomiccharacterizationofanewbiofilmformingandadhesivest398humanadaptedmssalineagecausingseptickneearthritisfollowingsurgicalreconstruction
AT borresilvio genomiccharacterizationofanewbiofilmformingandadhesivest398humanadaptedmssalineagecausingseptickneearthritisfollowingsurgicalreconstruction
AT stefanistefania genomiccharacterizationofanewbiofilmformingandadhesivest398humanadaptedmssalineagecausingseptickneearthritisfollowingsurgicalreconstruction