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The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling Pathway

Bluetongue virus (BTV), an arbovirus transmitted by Culicoides biting midges, is a major concern of wild and domestic ruminants. While BTV induces type I interferon (alpha/beta interferon [IFN-α/β]) production in infected cells, several reports have described evasion strategies elaborated by this vi...

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Autores principales: Pourcelot, Marie, Amaral Moraes, Rayane, Fablet, Aurore, Bréard, Emmanuel, Sailleau, Corinne, Viarouge, Cyril, Postic, Lydie, Zientara, Stéphan, Caignard, Grégory, Vitour, Damien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913109/
https://www.ncbi.nlm.nih.gov/pubmed/33540654
http://dx.doi.org/10.3390/v13020230
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author Pourcelot, Marie
Amaral Moraes, Rayane
Fablet, Aurore
Bréard, Emmanuel
Sailleau, Corinne
Viarouge, Cyril
Postic, Lydie
Zientara, Stéphan
Caignard, Grégory
Vitour, Damien
author_facet Pourcelot, Marie
Amaral Moraes, Rayane
Fablet, Aurore
Bréard, Emmanuel
Sailleau, Corinne
Viarouge, Cyril
Postic, Lydie
Zientara, Stéphan
Caignard, Grégory
Vitour, Damien
author_sort Pourcelot, Marie
collection PubMed
description Bluetongue virus (BTV), an arbovirus transmitted by Culicoides biting midges, is a major concern of wild and domestic ruminants. While BTV induces type I interferon (alpha/beta interferon [IFN-α/β]) production in infected cells, several reports have described evasion strategies elaborated by this virus to dampen this intrinsic, innate response. In the present study, we suggest that BTV VP3 is a new viral antagonist of the IFN-β synthesis. Indeed, using split luciferase and coprecipitation assays, we report an interaction between VP3 and both the mitochondrial adapter protein MAVS and the IRF3-kinase IKKε. Overall, this study describes a putative role for the BTV structural protein VP3 in the control of the antiviral response.
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spelling pubmed-79131092021-02-28 The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling Pathway Pourcelot, Marie Amaral Moraes, Rayane Fablet, Aurore Bréard, Emmanuel Sailleau, Corinne Viarouge, Cyril Postic, Lydie Zientara, Stéphan Caignard, Grégory Vitour, Damien Viruses Communication Bluetongue virus (BTV), an arbovirus transmitted by Culicoides biting midges, is a major concern of wild and domestic ruminants. While BTV induces type I interferon (alpha/beta interferon [IFN-α/β]) production in infected cells, several reports have described evasion strategies elaborated by this virus to dampen this intrinsic, innate response. In the present study, we suggest that BTV VP3 is a new viral antagonist of the IFN-β synthesis. Indeed, using split luciferase and coprecipitation assays, we report an interaction between VP3 and both the mitochondrial adapter protein MAVS and the IRF3-kinase IKKε. Overall, this study describes a putative role for the BTV structural protein VP3 in the control of the antiviral response. MDPI 2021-02-02 /pmc/articles/PMC7913109/ /pubmed/33540654 http://dx.doi.org/10.3390/v13020230 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Pourcelot, Marie
Amaral Moraes, Rayane
Fablet, Aurore
Bréard, Emmanuel
Sailleau, Corinne
Viarouge, Cyril
Postic, Lydie
Zientara, Stéphan
Caignard, Grégory
Vitour, Damien
The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling Pathway
title The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling Pathway
title_full The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling Pathway
title_fullStr The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling Pathway
title_full_unstemmed The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling Pathway
title_short The VP3 Protein of Bluetongue Virus Associates with the MAVS Complex and Interferes with the RIG-I-Signaling Pathway
title_sort vp3 protein of bluetongue virus associates with the mavs complex and interferes with the rig-i-signaling pathway
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913109/
https://www.ncbi.nlm.nih.gov/pubmed/33540654
http://dx.doi.org/10.3390/v13020230
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