Field Translocation of Mountain Pine Beetles Suggests Phoretic Mite Communities Are Locally Adapted, and Mite Populations Respond Variably to Climate Warming

SIMPLE SUMMARY: Climate warming has significant effects on forest insect populations, particularly bark beetles, which cause millions of hectares of forest tree damage. Bark beetles live alongside a diverse host of other organisms which affect the success of beetle attacks on trees and are also affected by climate changes. Here, we explore climate effects on symbiotic mite communities associated with the mountain pine beetle (Dendroctonus ponderosae). We show that warming causes significant shifts in the abundance of mites. These effects were dependent on source population, suggesting mite populations are adapted to their local climates. Understanding beetle–mite patterns is important because mites can directly affect beetle reproduction by feeding on eggs, or indirectly affect beetle health by introducing fungi. Our results provide foundational information for understanding how climate change will affect beetle–mite associations; and serve to help determine how these shifting associations will affect the success of bark beetles in forest ecosystems. ABSTRACT: Temperature is a key determining factor in the population dynamics of forest insects and their associated biota. Bark beetles, often considered key agents of change in forest ecosystems, are particularly affected by warming in their environment. Beetles associate with various phoretic mite species that have direct/indirect effects on beetle fitness and population dynamics, although there is limited knowledge of how temperature affects these communities. Here, we use a field reciprocal translocation experiment with the addition of a novel “warming” environment to represent future changes in local environment in two populations of a keystone bark beetle species (Dendroctonus ponderosae). We hypothesize that mite community abundances as carried by bark beetles are significantly altered when not in their native environments and when subjected to climate warming. We use multivariate generalized linear models based on species abundance data to show that mite community compositions significantly differ across different field climates; and that these patterns diverge between source populations, indicating local adaptation. Our study offers foundational information on the general effects of simulated climate-warming on the compositional shifts of common and abundant biotic associates of mountain pine beetles and may be used as a model system for other important insect–mite systems.