Cargando…

A Neutralization Assay Based on Pseudo-Typed Lentivirus with SARS CoV-2 Spike Protein in ACE2-Expressing CRFK Cells

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly pathogenic zoonotic virus that spreads rapidly. In this work, we improve the hitherto existing neutralization assay system to assess SARS-CoV-2 inhibitors using a pseudo-typed lentivirus coated with the SARS-CoV-2 spike protein...

Descripción completa

Detalles Bibliográficos
Autores principales:	Pısıl, Yalçın, Shida, Hisatoshi, Miura, Tomoyuki
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913246/ https://www.ncbi.nlm.nih.gov/pubmed/33540924 http://dx.doi.org/10.3390/pathogens10020153

Ejemplares similares

Is SARS-CoV-2 Neutralized More Effectively by IgM and IgA than IgG Having the Same Fab Region?
por: Pisil, Yalcin, et al.
Publicado: (2021)

Specific Substitutions in Region V2 of gp120 env confer SHIV Neutralisation Resistance
por: Pisil, Yalcin, et al.
Publicado: (2020)

Dynamic Assay for Profiling Anti-SARS-CoV-2 Antibodies and Their ACE2/Spike RBD Neutralization Capacity
por: Phelan, Thomas, et al.
Publicado: (2021)

Neutralization of SARS-CoV-2 spike pseudotyped virus by recombinant ACE2-Ig
por: Lei, Changhai, et al.
Publicado: (2020)

Validation and Establishment of the SARS-CoV-2 Lentivirus Surrogate Neutralization Assay as a Prescreening Tool for the Plaque Reduction Neutralization Test
por: Merluza, John, et al.
Publicado: (2023)

Effective screening of SARS-CoV-2 neutralizing antibodies in patient serum using lentivirus particles pseudotyped with SARS-CoV-2 spike glycoprotein
por: Tandon, Ritesh, et al.
Publicado: (2020)

The spike-ACE2 binding assay: An in vitro platform for evaluating vaccination efficacy and for screening SARS-CoV-2 inhibitors and neutralizing antibodies
por: Zhang, Shuangzhe, et al.
Publicado: (2022)

Dual-detection fluorescent immunochromatographic assay for quantitative detection of SARS-CoV-2 spike RBD-ACE2 blocking neutralizing antibody
por: Duan, Xuejun, et al.
Publicado: (2022)

Neutral evolution test of the spike protein of SARS-CoV-2 and its implications in the binding to ACE2
por: López-Cortés, Georgina I., et al.
Publicado: (2021)

Author Correction: Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2
por: Huo, Jiangdong, et al.
Publicado: (2020)

Synthetic carbohydrate-binding agents neutralize SARS-CoV-2 by inhibiting binding of the spike protein to ACE2
por: Francesconi, Oscar, et al.
Publicado: (2022)

Author Correction: Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2
por: Huo, Jiandong, et al.
Publicado: (2021)

Production of Recombinant Replication-defective Lentiviruses Bearing the SARS-CoV or SARS-CoV-2 Attachment Spike Glycoprotein and Their Application in Receptor Tropism and Neutralisation Assays
por: Thakur, Nazia, et al.
Publicado: (2021)

A bioluminescent and homogeneous SARS-CoV-2 spike RBD and hACE2 interaction assay for antiviral screening and monitoring patient neutralizing antibody levels
por: Alves, Juliano, et al.
Publicado: (2021)

Eukaryotic CRFK Cells Motion Characterized with Atomic Force Microscopy
por: Zamora-Ceballos, María, et al.
Publicado: (2022)

Could anti-ACE2 antibodies alter the results of SARS-CoV-2 Ab neutralization assays?
por: Tobes, Raquel, et al.
Publicado: (2022)

Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodies
por: Yi, Chunyan, et al.
Publicado: (2020)

A tetrameric ACE2 protein broadly neutralizes SARS-CoV-2 spike variants of concern with elevated potency
por: Leach, Adam, et al.
Publicado: (2021)

Rapid, reliable, and reproducible cell fusion assay to quantify SARS-Cov-2 spike interaction with hACE2
por: Zhao, Min, et al.
Publicado: (2021)

Identification of Closed Linear Epitopes in S1-RBD and S2-HR1/2 of SARS-CoV-2 Spike Protein Able to Induce Neutralizing Abs
por: Watanabe, Yoshihiro, et al.
Publicado: (2023)

Characterisation of Crandell-Rees Feline Kidney (CRFK) cells as mesenchymal in phenotype
por: Lawson, J.S., et al.
Publicado: (2019)

SARS-CoV-2 spike and ACE2 entanglement-like binding
por: Pregnolato, Massimo, et al.
Publicado: (2023)

Effects of Spike Mutations in SARS-CoV-2 Variants of Concern on Human or Animal ACE2-Mediated Virus Entry and Neutralization
por: Kim, Yunjeong, et al.
Publicado: (2021)

Effects of Spike Mutations in SARS-CoV-2 Variants of Concern on Human or Animal ACE2-Mediated Virus Entry and Neutralization
por: Kim, Yunjeong, et al.
Publicado: (2022)

Neutralization Assay with SARS-CoV-1 and SARS-CoV-2 Spike Pseudotyped Murine Leukemia Virions
por: Zheng, Yue, et al.
Publicado: (2020)

Neutralization assay with SARS-CoV-1 and SARS-CoV-2 spike pseudotyped murine leukemia virions
por: Zheng, Yue, et al.
Publicado: (2021)

Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays
por: Crawford, Katharine H. D., et al.
Publicado: (2020)

Spike Antibody Assays Can Screen For Viral Neutralizing Activity Toward SARS-CoV-2
por: Schuler, Charles, et al.
Publicado: (2022)

Deletion of the SARS-CoV-2 Spike Cytoplasmic Tail Increases Infectivity in Pseudovirus Neutralization Assays
por: Yu, Jingyou, et al.
Publicado: (2021)

Mutations in the SARS-CoV-2 spike RBD are responsible for stronger ACE2 binding and poor anti-SARS-CoV mAbs cross-neutralization
por: Shah, Masaud, et al.
Publicado: (2020)

Relative Ratios of Human Seasonal Coronavirus Antibodies Predict the Efficiency of Cross-Neutralization of SARS-CoV-2 Spike Binding to ACE2
por: Galipeau, Yannick, et al.
Publicado: (2021)

Correlation of SARS-CoV-2 Viral Neutralizing Antibody Titers with Anti-Spike Antibodies and ACE-2 Inhibition among Vaccinated Individuals
por: Grunau, Brian, et al.
Publicado: (2022)

SARS-CoV spike protein-expressing recombinant vaccinia virus efficiently induces neutralizing antibodies in rabbits pre-immunized with vaccinia virus
por: Kitabatake, Masahiro, et al.
Publicado: (2007)

Revealing the Mechanism of SARS-CoV-2 Spike Protein Binding With ACE2
Publicado: (2020)

SARS-CoV-2 electrochemical immunosensor based on the spike-ACE2 complex
por: Vásquez, Viviana, et al.
Publicado: (2022)

Mechanism and evolution of human ACE2 binding by SARS-CoV-2 spike
por: Wrobel, Antoni G.
Publicado: (2023)

Design and Identification of Inhibitors for the Spike-ACE2 Target of SARS-CoV-2
por: Bastos, Ruan S., et al.
Publicado: (2023)

CRFK and Primary Macrophages Transcriptomes in Response to Feline Coronavirus Infection Differ Significantly
por: Drechsler, Yvonne, et al.
Publicado: (2020)

Establishment of CRFK cells for vaccine production by inactivating endogenous retrovirus with TALEN technology
por: Shimode, Sayumi, et al.
Publicado: (2022)

Comprehensive engineering of a therapeutic neutralizing antibody targeting SARS-CoV-2 spike protein to neutralize escape variants
por: Kuramochi, Taichi, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_495q5fd0089fmb0bu59rp9cv9a