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Development of iRGD-Modified Peptide Carriers for Suicide Gene Therapy of Uterine Leiomyoma
Uterine leiomyoma (UL) is one of the most common benign tumors in women that often leads to many reproductive complications. Suicide genetherapy was suggested as a promising approach for UL treatment. In the present study, we describe iRGD ligand-conjugated cysteine-rich peptide carrier RGD1-R6 for...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913275/ https://www.ncbi.nlm.nih.gov/pubmed/33540912 http://dx.doi.org/10.3390/pharmaceutics13020202 |
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author | Egorova, Anna Shtykalova, Sofia Selutin, Alexander Shved, Natalia Maretina, Marianna Selkov, Sergei Baranov, Vladislav Kiselev, Anton |
author_facet | Egorova, Anna Shtykalova, Sofia Selutin, Alexander Shved, Natalia Maretina, Marianna Selkov, Sergei Baranov, Vladislav Kiselev, Anton |
author_sort | Egorova, Anna |
collection | PubMed |
description | Uterine leiomyoma (UL) is one of the most common benign tumors in women that often leads to many reproductive complications. Suicide genetherapy was suggested as a promising approach for UL treatment. In the present study, we describe iRGD ligand-conjugated cysteine-rich peptide carrier RGD1-R6 for targeted DNA delivery to αvβ3 integrin-expressing primary UL cells. The physico-chemical properties, cytotoxicity, transfection efficiency and specificity of DNA/RGD1-R6 polyplexes were investigated. TheHSV-1thymidine kinase encoding plasmid delivery to PANC-1pancreatic carcinoma cells and primary UL cells resulted in significant suicide gene therapy effects. Subsequent ganciclovir treatment decreased cells proliferative activity, induced of apoptosis and promoted cells death.The obtained results allow us to concludethatthe developed RGD1-R6 carrier can be considered a promising candidate for suicide gene therapy of uterine leiomyoma. |
format | Online Article Text |
id | pubmed-7913275 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79132752021-02-28 Development of iRGD-Modified Peptide Carriers for Suicide Gene Therapy of Uterine Leiomyoma Egorova, Anna Shtykalova, Sofia Selutin, Alexander Shved, Natalia Maretina, Marianna Selkov, Sergei Baranov, Vladislav Kiselev, Anton Pharmaceutics Article Uterine leiomyoma (UL) is one of the most common benign tumors in women that often leads to many reproductive complications. Suicide genetherapy was suggested as a promising approach for UL treatment. In the present study, we describe iRGD ligand-conjugated cysteine-rich peptide carrier RGD1-R6 for targeted DNA delivery to αvβ3 integrin-expressing primary UL cells. The physico-chemical properties, cytotoxicity, transfection efficiency and specificity of DNA/RGD1-R6 polyplexes were investigated. TheHSV-1thymidine kinase encoding plasmid delivery to PANC-1pancreatic carcinoma cells and primary UL cells resulted in significant suicide gene therapy effects. Subsequent ganciclovir treatment decreased cells proliferative activity, induced of apoptosis and promoted cells death.The obtained results allow us to concludethatthe developed RGD1-R6 carrier can be considered a promising candidate for suicide gene therapy of uterine leiomyoma. MDPI 2021-02-02 /pmc/articles/PMC7913275/ /pubmed/33540912 http://dx.doi.org/10.3390/pharmaceutics13020202 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Egorova, Anna Shtykalova, Sofia Selutin, Alexander Shved, Natalia Maretina, Marianna Selkov, Sergei Baranov, Vladislav Kiselev, Anton Development of iRGD-Modified Peptide Carriers for Suicide Gene Therapy of Uterine Leiomyoma |
title | Development of iRGD-Modified Peptide Carriers for Suicide Gene Therapy of Uterine Leiomyoma |
title_full | Development of iRGD-Modified Peptide Carriers for Suicide Gene Therapy of Uterine Leiomyoma |
title_fullStr | Development of iRGD-Modified Peptide Carriers for Suicide Gene Therapy of Uterine Leiomyoma |
title_full_unstemmed | Development of iRGD-Modified Peptide Carriers for Suicide Gene Therapy of Uterine Leiomyoma |
title_short | Development of iRGD-Modified Peptide Carriers for Suicide Gene Therapy of Uterine Leiomyoma |
title_sort | development of irgd-modified peptide carriers for suicide gene therapy of uterine leiomyoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913275/ https://www.ncbi.nlm.nih.gov/pubmed/33540912 http://dx.doi.org/10.3390/pharmaceutics13020202 |
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