Cargando…

Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts

Defatted rapeseed meal (DRM) is a sub-valorized agro-industrial by-product, with a high protein content whose peptides could have potential anticancer activity against cancer cell lines. The objective of the present study is to obtain an enzymatic hydrolysate of rapeseed protein that inhibits prolif...

Descripción completa

Detalles Bibliográficos
Autores principales: Ferrero, Romina L., Soto-Maldonado, Carmen, Weinstein-Oppenheimer, Caroline, Cabrera-Muñoz, Zaida, Zúñiga-Hansen, María Elvira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913290/
https://www.ncbi.nlm.nih.gov/pubmed/33546198
http://dx.doi.org/10.3390/foods10020309
_version_ 1783656770894299136
author Ferrero, Romina L.
Soto-Maldonado, Carmen
Weinstein-Oppenheimer, Caroline
Cabrera-Muñoz, Zaida
Zúñiga-Hansen, María Elvira
author_facet Ferrero, Romina L.
Soto-Maldonado, Carmen
Weinstein-Oppenheimer, Caroline
Cabrera-Muñoz, Zaida
Zúñiga-Hansen, María Elvira
author_sort Ferrero, Romina L.
collection PubMed
description Defatted rapeseed meal (DRM) is a sub-valorized agro-industrial by-product, with a high protein content whose peptides could have potential anticancer activity against cancer cell lines. The objective of the present study is to obtain an enzymatic hydrolysate of rapeseed protein that inhibits proliferation on a breast cancer cell line (MCF-7), but not healthy human fibroblast cells. The DRM was solubilized in an alkaline medium to obtain an alkaline rapeseed extract (RAE). Acid precipitation of the proteins contained in RAE recovered a rapeseed protein isolate (RPI). To produce protein hydrolysates, two alkaline protease and different enzyme/substrate ratios were used. All the protein hydrolysates showed antiproliferative activity on MCF-7 cells. However, only the hydrolysate recovered from the enzymatic hydrolysis of RPI (Degree of hydrolysis (DH ) between 8.5 and 9% (DH1)) did not affect human fibroblast cells, inhibiting 83.9% of MCF-7 cells’ proliferation and showing a mass yield of 22.9% (based on the initial DRM). The SDS-PAGE gel revealed that DH1 was composed mainly of 10 kDa peptides and, to a lesser extent, 5 and 2 kDa. It is concluded that DH1 is a promising peptide extract for future research as a putative anti-breast cancer agent.
format Online
Article
Text
id pubmed-7913290
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79132902021-02-28 Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts Ferrero, Romina L. Soto-Maldonado, Carmen Weinstein-Oppenheimer, Caroline Cabrera-Muñoz, Zaida Zúñiga-Hansen, María Elvira Foods Article Defatted rapeseed meal (DRM) is a sub-valorized agro-industrial by-product, with a high protein content whose peptides could have potential anticancer activity against cancer cell lines. The objective of the present study is to obtain an enzymatic hydrolysate of rapeseed protein that inhibits proliferation on a breast cancer cell line (MCF-7), but not healthy human fibroblast cells. The DRM was solubilized in an alkaline medium to obtain an alkaline rapeseed extract (RAE). Acid precipitation of the proteins contained in RAE recovered a rapeseed protein isolate (RPI). To produce protein hydrolysates, two alkaline protease and different enzyme/substrate ratios were used. All the protein hydrolysates showed antiproliferative activity on MCF-7 cells. However, only the hydrolysate recovered from the enzymatic hydrolysis of RPI (Degree of hydrolysis (DH ) between 8.5 and 9% (DH1)) did not affect human fibroblast cells, inhibiting 83.9% of MCF-7 cells’ proliferation and showing a mass yield of 22.9% (based on the initial DRM). The SDS-PAGE gel revealed that DH1 was composed mainly of 10 kDa peptides and, to a lesser extent, 5 and 2 kDa. It is concluded that DH1 is a promising peptide extract for future research as a putative anti-breast cancer agent. MDPI 2021-02-03 /pmc/articles/PMC7913290/ /pubmed/33546198 http://dx.doi.org/10.3390/foods10020309 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ferrero, Romina L.
Soto-Maldonado, Carmen
Weinstein-Oppenheimer, Caroline
Cabrera-Muñoz, Zaida
Zúñiga-Hansen, María Elvira
Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts
title Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts
title_full Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts
title_fullStr Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts
title_full_unstemmed Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts
title_short Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts
title_sort antiproliferative rapeseed defatted meal protein and their hydrolysates on mcf-7 breast cancer cells and human fibroblasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913290/
https://www.ncbi.nlm.nih.gov/pubmed/33546198
http://dx.doi.org/10.3390/foods10020309
work_keys_str_mv AT ferrerorominal antiproliferativerapeseeddefattedmealproteinandtheirhydrolysatesonmcf7breastcancercellsandhumanfibroblasts
AT sotomaldonadocarmen antiproliferativerapeseeddefattedmealproteinandtheirhydrolysatesonmcf7breastcancercellsandhumanfibroblasts
AT weinsteinoppenheimercaroline antiproliferativerapeseeddefattedmealproteinandtheirhydrolysatesonmcf7breastcancercellsandhumanfibroblasts
AT cabreramunozzaida antiproliferativerapeseeddefattedmealproteinandtheirhydrolysatesonmcf7breastcancercellsandhumanfibroblasts
AT zunigahansenmariaelvira antiproliferativerapeseeddefattedmealproteinandtheirhydrolysatesonmcf7breastcancercellsandhumanfibroblasts