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Long-Range PCR-Based NGS Applications to Diagnose Mendelian Retinal Diseases

The purpose of this study was to develop a flexible, cost-efficient, next-generation sequencing (NGS) protocol for genetic testing. Long-range polymerase chain reaction (PCR) amplicons of up to 20 kb in size were designed to amplify entire genomic regions for a panel (n = 35) of inherited retinal di...

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Detalles Bibliográficos
Autores principales:	Maggi, Jordi, Koller, Samuel, Bähr, Luzy, Feil, Silke, Kivrak Pfiffner, Fatma, Hanson, James V. M., Maspoli, Alessandro, Gerth-Kahlert, Christina, Berger, Wolfgang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913364/ https://www.ncbi.nlm.nih.gov/pubmed/33546218 http://dx.doi.org/10.3390/ijms22041508

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