Cargando…
The Rice Small Auxin-Up RNA Gene OsSAUR33 Regulates Seed Vigor via Sugar Pathway during Early Seed Germination
Seed vigor affects seed germination and seedling emergence, and therefore is an important agronomic trait in rice. Small auxin-up RNAs (SAURs) function in a range of developmental processes, but their role in seed vigor remains unclear. Here, we observed that disruption of OsSAUR33 resulted in reduc...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913900/ https://www.ncbi.nlm.nih.gov/pubmed/33557166 http://dx.doi.org/10.3390/ijms22041562 |
_version_ | 1783656909776093184 |
---|---|
author | Zhao, Jia Li, Wenjun Sun, Shan Peng, Liling Huang, Zhibo He, Yongqi Wang, Zhoufei |
author_facet | Zhao, Jia Li, Wenjun Sun, Shan Peng, Liling Huang, Zhibo He, Yongqi Wang, Zhoufei |
author_sort | Zhao, Jia |
collection | PubMed |
description | Seed vigor affects seed germination and seedling emergence, and therefore is an important agronomic trait in rice. Small auxin-up RNAs (SAURs) function in a range of developmental processes, but their role in seed vigor remains unclear. Here, we observed that disruption of OsSAUR33 resulted in reduced germination rates and low seed uniformity in early germination. Expression of OsSAUR33 was higher in mature grains and early germinating seeds. RNA-seq analysis revealed that OsSAUR33 modulated seed vigor by affecting the mobilization of stored reserves during germination. Disruption of OsSAUR33 increased the soluble sugar content in dry mature grains and seeds during early germination. OsSAUR33 interacted with the sucrose non-fermenting-1-related protein kinase OsSnRK1A, a regulator of the sugar signaling pathway, which influences the expression of sugar signaling-related genes during germination. Disruption of OsSAUR33 increased sugar-sensitive phenotypes in early germination, suggesting OsSAUR33 likely affects seed vigor through the sugar pathway. One elite haplotype of OsSAUR33 associated with higher seed vigor was identified mainly in indica accessions. This study provides insight into the effects of OsSAUR33 on seed vigor in rice. |
format | Online Article Text |
id | pubmed-7913900 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79139002021-02-28 The Rice Small Auxin-Up RNA Gene OsSAUR33 Regulates Seed Vigor via Sugar Pathway during Early Seed Germination Zhao, Jia Li, Wenjun Sun, Shan Peng, Liling Huang, Zhibo He, Yongqi Wang, Zhoufei Int J Mol Sci Article Seed vigor affects seed germination and seedling emergence, and therefore is an important agronomic trait in rice. Small auxin-up RNAs (SAURs) function in a range of developmental processes, but their role in seed vigor remains unclear. Here, we observed that disruption of OsSAUR33 resulted in reduced germination rates and low seed uniformity in early germination. Expression of OsSAUR33 was higher in mature grains and early germinating seeds. RNA-seq analysis revealed that OsSAUR33 modulated seed vigor by affecting the mobilization of stored reserves during germination. Disruption of OsSAUR33 increased the soluble sugar content in dry mature grains and seeds during early germination. OsSAUR33 interacted with the sucrose non-fermenting-1-related protein kinase OsSnRK1A, a regulator of the sugar signaling pathway, which influences the expression of sugar signaling-related genes during germination. Disruption of OsSAUR33 increased sugar-sensitive phenotypes in early germination, suggesting OsSAUR33 likely affects seed vigor through the sugar pathway. One elite haplotype of OsSAUR33 associated with higher seed vigor was identified mainly in indica accessions. This study provides insight into the effects of OsSAUR33 on seed vigor in rice. MDPI 2021-02-04 /pmc/articles/PMC7913900/ /pubmed/33557166 http://dx.doi.org/10.3390/ijms22041562 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhao, Jia Li, Wenjun Sun, Shan Peng, Liling Huang, Zhibo He, Yongqi Wang, Zhoufei The Rice Small Auxin-Up RNA Gene OsSAUR33 Regulates Seed Vigor via Sugar Pathway during Early Seed Germination |
title | The Rice Small Auxin-Up RNA Gene OsSAUR33 Regulates Seed Vigor via Sugar Pathway during Early Seed Germination |
title_full | The Rice Small Auxin-Up RNA Gene OsSAUR33 Regulates Seed Vigor via Sugar Pathway during Early Seed Germination |
title_fullStr | The Rice Small Auxin-Up RNA Gene OsSAUR33 Regulates Seed Vigor via Sugar Pathway during Early Seed Germination |
title_full_unstemmed | The Rice Small Auxin-Up RNA Gene OsSAUR33 Regulates Seed Vigor via Sugar Pathway during Early Seed Germination |
title_short | The Rice Small Auxin-Up RNA Gene OsSAUR33 Regulates Seed Vigor via Sugar Pathway during Early Seed Germination |
title_sort | rice small auxin-up rna gene ossaur33 regulates seed vigor via sugar pathway during early seed germination |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913900/ https://www.ncbi.nlm.nih.gov/pubmed/33557166 http://dx.doi.org/10.3390/ijms22041562 |
work_keys_str_mv | AT zhaojia thericesmallauxinuprnageneossaur33regulatesseedvigorviasugarpathwayduringearlyseedgermination AT liwenjun thericesmallauxinuprnageneossaur33regulatesseedvigorviasugarpathwayduringearlyseedgermination AT sunshan thericesmallauxinuprnageneossaur33regulatesseedvigorviasugarpathwayduringearlyseedgermination AT pengliling thericesmallauxinuprnageneossaur33regulatesseedvigorviasugarpathwayduringearlyseedgermination AT huangzhibo thericesmallauxinuprnageneossaur33regulatesseedvigorviasugarpathwayduringearlyseedgermination AT heyongqi thericesmallauxinuprnageneossaur33regulatesseedvigorviasugarpathwayduringearlyseedgermination AT wangzhoufei thericesmallauxinuprnageneossaur33regulatesseedvigorviasugarpathwayduringearlyseedgermination AT zhaojia ricesmallauxinuprnageneossaur33regulatesseedvigorviasugarpathwayduringearlyseedgermination AT liwenjun ricesmallauxinuprnageneossaur33regulatesseedvigorviasugarpathwayduringearlyseedgermination AT sunshan ricesmallauxinuprnageneossaur33regulatesseedvigorviasugarpathwayduringearlyseedgermination AT pengliling ricesmallauxinuprnageneossaur33regulatesseedvigorviasugarpathwayduringearlyseedgermination AT huangzhibo ricesmallauxinuprnageneossaur33regulatesseedvigorviasugarpathwayduringearlyseedgermination AT heyongqi ricesmallauxinuprnageneossaur33regulatesseedvigorviasugarpathwayduringearlyseedgermination AT wangzhoufei ricesmallauxinuprnageneossaur33regulatesseedvigorviasugarpathwayduringearlyseedgermination |