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GC-MS Characterization of Antibacterial, Antioxidant, and Antitrypanosomal Activity of Syzygium aromaticum Essential Oil and Eugenol
Syzygium aromaticum has a diversity of biological activities due to the chemical compounds found in its plant products such as total phenolic compounds and flavonoids. The present work describes the chemical analysis and antimicrobial, antioxidant, and antitrypanosomal activity of the essential oil...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914077/ https://www.ncbi.nlm.nih.gov/pubmed/33688364 http://dx.doi.org/10.1155/2021/6663255 |
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author | Teles, Amanda Mara Silva-Silva, João Victor Fernandes, Juan Matheus Pereira Abreu-Silva, Ana Lucia Calabrese, Kátia da Silva Mendes Filho, Nestor Everton Mouchrek, Adenilde Nascimento Almeida-Souza, Fernando |
author_facet | Teles, Amanda Mara Silva-Silva, João Victor Fernandes, Juan Matheus Pereira Abreu-Silva, Ana Lucia Calabrese, Kátia da Silva Mendes Filho, Nestor Everton Mouchrek, Adenilde Nascimento Almeida-Souza, Fernando |
author_sort | Teles, Amanda Mara |
collection | PubMed |
description | Syzygium aromaticum has a diversity of biological activities due to the chemical compounds found in its plant products such as total phenolic compounds and flavonoids. The present work describes the chemical analysis and antimicrobial, antioxidant, and antitrypanosomal activity of the essential oil of S. aromaticum. Eugenol (53.23%) as the major compound was verified by gas chromatography-mass spectrometry. S. aromaticum essential oil was more effective against S. aureus (MIC 50 μg/mL) than eugenol (MIC 250 μg/mL). Eugenol presented higher antioxidant activity than S. aromaticum essential oil, with an EC(50) of 12.66 and 78.98 µg/mL, respectively. S. aromaticum essential oil and eugenol exhibited Trypanosoma cruzi inhibitory activity, with IC(50) of 28.68 ± 1.073 and 31.97 ± 1.061 μg/mL against epimastigotes and IC(50) of 64.51 ± 1.658 and 45.73 ± 1.252 μg/mL against intracellular amastigotes, respectively. Both compounds presented low cytotoxicity, with S. aromaticum essential oil displaying 15.5-fold greater selectivity for the parasite than the cells. Nitrite levels in T. cruzi-stimulated cells were reduced by essential oil (47.01%; p = 0.002) and eugenol (48.05%; p = 0.003) treatment. The trypanocidal activity of S. aromaticum essential oil showed that it is reasonable to use it in future research in the search for new therapeutic alternatives for trypanosomiasis. |
format | Online Article Text |
id | pubmed-7914077 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-79140772021-03-08 GC-MS Characterization of Antibacterial, Antioxidant, and Antitrypanosomal Activity of Syzygium aromaticum Essential Oil and Eugenol Teles, Amanda Mara Silva-Silva, João Victor Fernandes, Juan Matheus Pereira Abreu-Silva, Ana Lucia Calabrese, Kátia da Silva Mendes Filho, Nestor Everton Mouchrek, Adenilde Nascimento Almeida-Souza, Fernando Evid Based Complement Alternat Med Research Article Syzygium aromaticum has a diversity of biological activities due to the chemical compounds found in its plant products such as total phenolic compounds and flavonoids. The present work describes the chemical analysis and antimicrobial, antioxidant, and antitrypanosomal activity of the essential oil of S. aromaticum. Eugenol (53.23%) as the major compound was verified by gas chromatography-mass spectrometry. S. aromaticum essential oil was more effective against S. aureus (MIC 50 μg/mL) than eugenol (MIC 250 μg/mL). Eugenol presented higher antioxidant activity than S. aromaticum essential oil, with an EC(50) of 12.66 and 78.98 µg/mL, respectively. S. aromaticum essential oil and eugenol exhibited Trypanosoma cruzi inhibitory activity, with IC(50) of 28.68 ± 1.073 and 31.97 ± 1.061 μg/mL against epimastigotes and IC(50) of 64.51 ± 1.658 and 45.73 ± 1.252 μg/mL against intracellular amastigotes, respectively. Both compounds presented low cytotoxicity, with S. aromaticum essential oil displaying 15.5-fold greater selectivity for the parasite than the cells. Nitrite levels in T. cruzi-stimulated cells were reduced by essential oil (47.01%; p = 0.002) and eugenol (48.05%; p = 0.003) treatment. The trypanocidal activity of S. aromaticum essential oil showed that it is reasonable to use it in future research in the search for new therapeutic alternatives for trypanosomiasis. Hindawi 2021-02-20 /pmc/articles/PMC7914077/ /pubmed/33688364 http://dx.doi.org/10.1155/2021/6663255 Text en Copyright © 2021 Amanda Mara Teles et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Teles, Amanda Mara Silva-Silva, João Victor Fernandes, Juan Matheus Pereira Abreu-Silva, Ana Lucia Calabrese, Kátia da Silva Mendes Filho, Nestor Everton Mouchrek, Adenilde Nascimento Almeida-Souza, Fernando GC-MS Characterization of Antibacterial, Antioxidant, and Antitrypanosomal Activity of Syzygium aromaticum Essential Oil and Eugenol |
title | GC-MS Characterization of Antibacterial, Antioxidant, and Antitrypanosomal Activity of Syzygium aromaticum Essential Oil and Eugenol |
title_full | GC-MS Characterization of Antibacterial, Antioxidant, and Antitrypanosomal Activity of Syzygium aromaticum Essential Oil and Eugenol |
title_fullStr | GC-MS Characterization of Antibacterial, Antioxidant, and Antitrypanosomal Activity of Syzygium aromaticum Essential Oil and Eugenol |
title_full_unstemmed | GC-MS Characterization of Antibacterial, Antioxidant, and Antitrypanosomal Activity of Syzygium aromaticum Essential Oil and Eugenol |
title_short | GC-MS Characterization of Antibacterial, Antioxidant, and Antitrypanosomal Activity of Syzygium aromaticum Essential Oil and Eugenol |
title_sort | gc-ms characterization of antibacterial, antioxidant, and antitrypanosomal activity of syzygium aromaticum essential oil and eugenol |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914077/ https://www.ncbi.nlm.nih.gov/pubmed/33688364 http://dx.doi.org/10.1155/2021/6663255 |
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