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Oxygen content-related DNA damage of graphene oxide on human retinal pigment epithelium cells

Arguments regarding the biocompatibility of graphene-based materials (GBMs) have never ceased. Particularly, the genotoxicity (e.g., DNA damage) of GBMs has been considered the greatest risk to healthy cells. Detailed genotoxicity studies of GBMs are necessary and essential. Herein, we present our r...

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Autores principales: Ou, Liling, Lv, Xiujuan, Wu, Zixia, Xia, Weibo, Huang, Yida, Chen, Luya, Sun, Wenjie, Qi, Yao, Yang, Mei, Qi, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914238/
https://www.ncbi.nlm.nih.gov/pubmed/33638700
http://dx.doi.org/10.1007/s10856-021-06491-0
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author Ou, Liling
Lv, Xiujuan
Wu, Zixia
Xia, Weibo
Huang, Yida
Chen, Luya
Sun, Wenjie
Qi, Yao
Yang, Mei
Qi, Lei
author_facet Ou, Liling
Lv, Xiujuan
Wu, Zixia
Xia, Weibo
Huang, Yida
Chen, Luya
Sun, Wenjie
Qi, Yao
Yang, Mei
Qi, Lei
author_sort Ou, Liling
collection PubMed
description Arguments regarding the biocompatibility of graphene-based materials (GBMs) have never ceased. Particularly, the genotoxicity (e.g., DNA damage) of GBMs has been considered the greatest risk to healthy cells. Detailed genotoxicity studies of GBMs are necessary and essential. Herein, we present our recent studies on the genotoxicity of most widely used GBMs such as graphene oxide (GO) and the chemically reduced graphene oxide (RGO) toward human retinal pigment epithelium (RPE) cells. The genotoxicity of GO and RGOs against ARPE-19 (a typical RPE cell line) cells was investigated using the alkaline comet assay, the expression level of phosphorylated p53 determined via Western blots, and the release level of reactive oxygen species (ROS). Our results suggested that both GO and RGOs induced ROS-dependent DNA damage. However, the DNA damage was enhanced following the reduction of the saturated C–O bonds in GO, suggesting that surface oxygen-containing groups played essential roles in the reduced genotoxicity of graphene and had the potential possibility to reduce the toxicity of GBMs via chemical modification. [Image: see text]
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spelling pubmed-79142382021-03-15 Oxygen content-related DNA damage of graphene oxide on human retinal pigment epithelium cells Ou, Liling Lv, Xiujuan Wu, Zixia Xia, Weibo Huang, Yida Chen, Luya Sun, Wenjie Qi, Yao Yang, Mei Qi, Lei J Mater Sci Mater Med S.I.: Biomaterial-Tissue Interaction in Humans Arguments regarding the biocompatibility of graphene-based materials (GBMs) have never ceased. Particularly, the genotoxicity (e.g., DNA damage) of GBMs has been considered the greatest risk to healthy cells. Detailed genotoxicity studies of GBMs are necessary and essential. Herein, we present our recent studies on the genotoxicity of most widely used GBMs such as graphene oxide (GO) and the chemically reduced graphene oxide (RGO) toward human retinal pigment epithelium (RPE) cells. The genotoxicity of GO and RGOs against ARPE-19 (a typical RPE cell line) cells was investigated using the alkaline comet assay, the expression level of phosphorylated p53 determined via Western blots, and the release level of reactive oxygen species (ROS). Our results suggested that both GO and RGOs induced ROS-dependent DNA damage. However, the DNA damage was enhanced following the reduction of the saturated C–O bonds in GO, suggesting that surface oxygen-containing groups played essential roles in the reduced genotoxicity of graphene and had the potential possibility to reduce the toxicity of GBMs via chemical modification. [Image: see text] Springer US 2021-02-27 2021 /pmc/articles/PMC7914238/ /pubmed/33638700 http://dx.doi.org/10.1007/s10856-021-06491-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle S.I.: Biomaterial-Tissue Interaction in Humans
Ou, Liling
Lv, Xiujuan
Wu, Zixia
Xia, Weibo
Huang, Yida
Chen, Luya
Sun, Wenjie
Qi, Yao
Yang, Mei
Qi, Lei
Oxygen content-related DNA damage of graphene oxide on human retinal pigment epithelium cells
title Oxygen content-related DNA damage of graphene oxide on human retinal pigment epithelium cells
title_full Oxygen content-related DNA damage of graphene oxide on human retinal pigment epithelium cells
title_fullStr Oxygen content-related DNA damage of graphene oxide on human retinal pigment epithelium cells
title_full_unstemmed Oxygen content-related DNA damage of graphene oxide on human retinal pigment epithelium cells
title_short Oxygen content-related DNA damage of graphene oxide on human retinal pigment epithelium cells
title_sort oxygen content-related dna damage of graphene oxide on human retinal pigment epithelium cells
topic S.I.: Biomaterial-Tissue Interaction in Humans
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914238/
https://www.ncbi.nlm.nih.gov/pubmed/33638700
http://dx.doi.org/10.1007/s10856-021-06491-0
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