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Central cholinergic synapse formation in optimized primary septal-hippocampal co-cultures
Septal innervation of basal forebrain cholinergic neurons to the hippocampus is critical for normal learning and memory and is severely degenerated in Alzheimer’s disease. To understand the molecular events underlying physiological cholinergic synaptogenesis and remodeling, as well as pathological l...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914286/ https://www.ncbi.nlm.nih.gov/pubmed/32860154 http://dx.doi.org/10.1007/s10571-020-00948-6 |
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author | Djemil, Sarra Ressel, Claire R. Abdel-Ghani, Mai Schneeweis, Amanda K Pak, Daniel T.S. |
author_facet | Djemil, Sarra Ressel, Claire R. Abdel-Ghani, Mai Schneeweis, Amanda K Pak, Daniel T.S. |
author_sort | Djemil, Sarra |
collection | PubMed |
description | Septal innervation of basal forebrain cholinergic neurons to the hippocampus is critical for normal learning and memory and is severely degenerated in Alzheimer’s disease. To understand the molecular events underlying physiological cholinergic synaptogenesis and remodeling, as well as pathological loss, we developed an optimized primary septal-hippocampal co-culture system. Hippocampal and septal tissue were harvested from embryonic Sprague-Dawley rat brain and cultured together at varying densities, cell ratios, and in the presence of different growth factors. We identified conditions that produced robust septal-hippocampal synapse formation. We used confocal microscopy with primary antibodies and fluorescent ligands to validate that this system was capable of generating developmentally mature cholinergic synapses. Such synapses were comprised of physiological synaptic partners and mimicked the molecular composition of in vivo counterparts. This co-culture system will facilitate the study of the formation, plasticity, and dysfunction of central mammalian cholinergic synapses. |
format | Online Article Text |
id | pubmed-7914286 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
record_format | MEDLINE/PubMed |
spelling | pubmed-79142862022-11-01 Central cholinergic synapse formation in optimized primary septal-hippocampal co-cultures Djemil, Sarra Ressel, Claire R. Abdel-Ghani, Mai Schneeweis, Amanda K Pak, Daniel T.S. Cell Mol Neurobiol Article Septal innervation of basal forebrain cholinergic neurons to the hippocampus is critical for normal learning and memory and is severely degenerated in Alzheimer’s disease. To understand the molecular events underlying physiological cholinergic synaptogenesis and remodeling, as well as pathological loss, we developed an optimized primary septal-hippocampal co-culture system. Hippocampal and septal tissue were harvested from embryonic Sprague-Dawley rat brain and cultured together at varying densities, cell ratios, and in the presence of different growth factors. We identified conditions that produced robust septal-hippocampal synapse formation. We used confocal microscopy with primary antibodies and fluorescent ligands to validate that this system was capable of generating developmentally mature cholinergic synapses. Such synapses were comprised of physiological synaptic partners and mimicked the molecular composition of in vivo counterparts. This co-culture system will facilitate the study of the formation, plasticity, and dysfunction of central mammalian cholinergic synapses. 2020-08-28 2021-11 /pmc/articles/PMC7914286/ /pubmed/32860154 http://dx.doi.org/10.1007/s10571-020-00948-6 Text en https://creativecommons.org/licenses/by/4.0/Terms of use and reuse: academic research for non-commercial purposes, see here for full terms. https://www.springer.com/aam-terms-v1 |
spellingShingle | Article Djemil, Sarra Ressel, Claire R. Abdel-Ghani, Mai Schneeweis, Amanda K Pak, Daniel T.S. Central cholinergic synapse formation in optimized primary septal-hippocampal co-cultures |
title | Central cholinergic synapse formation in optimized primary septal-hippocampal co-cultures |
title_full | Central cholinergic synapse formation in optimized primary septal-hippocampal co-cultures |
title_fullStr | Central cholinergic synapse formation in optimized primary septal-hippocampal co-cultures |
title_full_unstemmed | Central cholinergic synapse formation in optimized primary septal-hippocampal co-cultures |
title_short | Central cholinergic synapse formation in optimized primary septal-hippocampal co-cultures |
title_sort | central cholinergic synapse formation in optimized primary septal-hippocampal co-cultures |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914286/ https://www.ncbi.nlm.nih.gov/pubmed/32860154 http://dx.doi.org/10.1007/s10571-020-00948-6 |
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