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Novel Fluorinated Poly (Lactic-Co-Glycolic acid) (PLGA) and Polyethylene Glycol (PEG) Nanoparticles for Monitoring and Imaging in Osteoarthritis

Polymeric nanoparticles (NPs) find many uses in nanomedicine, from drug delivery to imaging. In this regard, poly (lactic-co-glycolic acid) (PLGA) and polyethylene glycol (PEG) particles are the most widely applied types of nano-systems due to their biocompatibility and biodegradability. Here we dev...

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Autores principales: Zerrillo, Luana, Gupta, Karthick Babu Sai Sankar, Lefeber, Fons A.W.M., Da Silva, Candido G., Galli, Federica, Chan, Alan, Veltien, Andor, Dou, Weiqiang, Censi, Roberta, Di Martino, Piera, Srinivas, Mangala, Cruz, Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914518/
https://www.ncbi.nlm.nih.gov/pubmed/33562356
http://dx.doi.org/10.3390/pharmaceutics13020235
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author Zerrillo, Luana
Gupta, Karthick Babu Sai Sankar
Lefeber, Fons A.W.M.
Da Silva, Candido G.
Galli, Federica
Chan, Alan
Veltien, Andor
Dou, Weiqiang
Censi, Roberta
Di Martino, Piera
Srinivas, Mangala
Cruz, Luis
author_facet Zerrillo, Luana
Gupta, Karthick Babu Sai Sankar
Lefeber, Fons A.W.M.
Da Silva, Candido G.
Galli, Federica
Chan, Alan
Veltien, Andor
Dou, Weiqiang
Censi, Roberta
Di Martino, Piera
Srinivas, Mangala
Cruz, Luis
author_sort Zerrillo, Luana
collection PubMed
description Polymeric nanoparticles (NPs) find many uses in nanomedicine, from drug delivery to imaging. In this regard, poly (lactic-co-glycolic acid) (PLGA) and polyethylene glycol (PEG) particles are the most widely applied types of nano-systems due to their biocompatibility and biodegradability. Here we developed novel fluorinated polymeric NPs as vectors for multi-modal nanoprobes. This approach involved modifying polymeric NPs with trifluoroacetamide (TFA) and loading them with a near-infrared (NIR) dye for different imaging modalities, such as magnetic resonance imaging (MRI) and optical imaging. The PLGA-PEG-TFA NPs generated were characterized in vitro using the C28/I2 human chondrocyte cell line and in vivo in a mouse model of osteoarthritis (OA). The NPs were well absorbed, as confirmed by confocal microscopy, and were non-toxic to cells. To test the NPs as a drug delivery system for contrast agents of OA, the nanomaterial was administered via the intra-articular (IA) administration method. The dye-loaded NPs were injected in the knee joint and then visualized and tracked in vivo by fluorine-19 nuclear magnetic resonance and fluorescence imaging. Here, we describe the development of novel intrinsically fluorinated polymeric NPs modality that can be used in various molecular imaging techniques to visualize and track OA treatments and their potential use in clinical trials.
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spelling pubmed-79145182021-03-01 Novel Fluorinated Poly (Lactic-Co-Glycolic acid) (PLGA) and Polyethylene Glycol (PEG) Nanoparticles for Monitoring and Imaging in Osteoarthritis Zerrillo, Luana Gupta, Karthick Babu Sai Sankar Lefeber, Fons A.W.M. Da Silva, Candido G. Galli, Federica Chan, Alan Veltien, Andor Dou, Weiqiang Censi, Roberta Di Martino, Piera Srinivas, Mangala Cruz, Luis Pharmaceutics Article Polymeric nanoparticles (NPs) find many uses in nanomedicine, from drug delivery to imaging. In this regard, poly (lactic-co-glycolic acid) (PLGA) and polyethylene glycol (PEG) particles are the most widely applied types of nano-systems due to their biocompatibility and biodegradability. Here we developed novel fluorinated polymeric NPs as vectors for multi-modal nanoprobes. This approach involved modifying polymeric NPs with trifluoroacetamide (TFA) and loading them with a near-infrared (NIR) dye for different imaging modalities, such as magnetic resonance imaging (MRI) and optical imaging. The PLGA-PEG-TFA NPs generated were characterized in vitro using the C28/I2 human chondrocyte cell line and in vivo in a mouse model of osteoarthritis (OA). The NPs were well absorbed, as confirmed by confocal microscopy, and were non-toxic to cells. To test the NPs as a drug delivery system for contrast agents of OA, the nanomaterial was administered via the intra-articular (IA) administration method. The dye-loaded NPs were injected in the knee joint and then visualized and tracked in vivo by fluorine-19 nuclear magnetic resonance and fluorescence imaging. Here, we describe the development of novel intrinsically fluorinated polymeric NPs modality that can be used in various molecular imaging techniques to visualize and track OA treatments and their potential use in clinical trials. MDPI 2021-02-07 /pmc/articles/PMC7914518/ /pubmed/33562356 http://dx.doi.org/10.3390/pharmaceutics13020235 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zerrillo, Luana
Gupta, Karthick Babu Sai Sankar
Lefeber, Fons A.W.M.
Da Silva, Candido G.
Galli, Federica
Chan, Alan
Veltien, Andor
Dou, Weiqiang
Censi, Roberta
Di Martino, Piera
Srinivas, Mangala
Cruz, Luis
Novel Fluorinated Poly (Lactic-Co-Glycolic acid) (PLGA) and Polyethylene Glycol (PEG) Nanoparticles for Monitoring and Imaging in Osteoarthritis
title Novel Fluorinated Poly (Lactic-Co-Glycolic acid) (PLGA) and Polyethylene Glycol (PEG) Nanoparticles for Monitoring and Imaging in Osteoarthritis
title_full Novel Fluorinated Poly (Lactic-Co-Glycolic acid) (PLGA) and Polyethylene Glycol (PEG) Nanoparticles for Monitoring and Imaging in Osteoarthritis
title_fullStr Novel Fluorinated Poly (Lactic-Co-Glycolic acid) (PLGA) and Polyethylene Glycol (PEG) Nanoparticles for Monitoring and Imaging in Osteoarthritis
title_full_unstemmed Novel Fluorinated Poly (Lactic-Co-Glycolic acid) (PLGA) and Polyethylene Glycol (PEG) Nanoparticles for Monitoring and Imaging in Osteoarthritis
title_short Novel Fluorinated Poly (Lactic-Co-Glycolic acid) (PLGA) and Polyethylene Glycol (PEG) Nanoparticles for Monitoring and Imaging in Osteoarthritis
title_sort novel fluorinated poly (lactic-co-glycolic acid) (plga) and polyethylene glycol (peg) nanoparticles for monitoring and imaging in osteoarthritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914518/
https://www.ncbi.nlm.nih.gov/pubmed/33562356
http://dx.doi.org/10.3390/pharmaceutics13020235
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