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Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism

Despite the scientific evidence supporting their biostimulant activity, the molecular mechanism(s) underlying the activity of protein hydrolysates (PHs) and the specificity among different products are still poorly explored. This work tested five different protein hydrolysates, produced from differe...

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Autores principales: Ceccarelli, Angela Valentina, Miras-Moreno, Begoña, Buffagni, Valentina, Senizza, Biancamaria, Pii, Youry, Cardarelli, Mariateresa, Rouphael, Youssef, Colla, Giuseppe, Lucini, Luigi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914860/
https://www.ncbi.nlm.nih.gov/pubmed/33567668
http://dx.doi.org/10.3390/plants10020326
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author Ceccarelli, Angela Valentina
Miras-Moreno, Begoña
Buffagni, Valentina
Senizza, Biancamaria
Pii, Youry
Cardarelli, Mariateresa
Rouphael, Youssef
Colla, Giuseppe
Lucini, Luigi
author_facet Ceccarelli, Angela Valentina
Miras-Moreno, Begoña
Buffagni, Valentina
Senizza, Biancamaria
Pii, Youry
Cardarelli, Mariateresa
Rouphael, Youssef
Colla, Giuseppe
Lucini, Luigi
author_sort Ceccarelli, Angela Valentina
collection PubMed
description Despite the scientific evidence supporting their biostimulant activity, the molecular mechanism(s) underlying the activity of protein hydrolysates (PHs) and the specificity among different products are still poorly explored. This work tested five different protein hydrolysates, produced from different plant sources using the same enzymatic approach, for their ability to promote rooting in tomato cuttings following quick dipping. Provided that all the different PHs increased root length (45–93%) and some of them increased root number (37–56%), untargeted metabolomics followed by multivariate statistics and pathway analysis were used to unravel the molecular processes at the basis of the biostimulant activity. Distinct metabolomic signatures could be found in roots following the PHs treatments. In general, PHs shaped the phytohormone profile, modulating the complex interaction between cytokinins and auxins, an interplay playing a pivotal role in root development, and triggered a down accumulation of brassinosteroids. Concerning secondary metabolism, PHs induced the accumulation of aliphatic glucosinolates, alkaloids, and phenylpropanoids, potentially eliciting crop resilience to stress conditions. Here, we confirm that PHs may have a hormone-like activity, and that their application can modulate plant growth, likely interfering with signaling processes. Noteworthy, the heterogenicity of the botanical origin supported the distinctive and peculiar metabolomic responses we observed across the products tested. While supporting their biostimulant activity, these findings suggest that a generalized crop response to PHs cannot be defined and that specific effects are rather to be investigated.
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spelling pubmed-79148602021-03-01 Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism Ceccarelli, Angela Valentina Miras-Moreno, Begoña Buffagni, Valentina Senizza, Biancamaria Pii, Youry Cardarelli, Mariateresa Rouphael, Youssef Colla, Giuseppe Lucini, Luigi Plants (Basel) Article Despite the scientific evidence supporting their biostimulant activity, the molecular mechanism(s) underlying the activity of protein hydrolysates (PHs) and the specificity among different products are still poorly explored. This work tested five different protein hydrolysates, produced from different plant sources using the same enzymatic approach, for their ability to promote rooting in tomato cuttings following quick dipping. Provided that all the different PHs increased root length (45–93%) and some of them increased root number (37–56%), untargeted metabolomics followed by multivariate statistics and pathway analysis were used to unravel the molecular processes at the basis of the biostimulant activity. Distinct metabolomic signatures could be found in roots following the PHs treatments. In general, PHs shaped the phytohormone profile, modulating the complex interaction between cytokinins and auxins, an interplay playing a pivotal role in root development, and triggered a down accumulation of brassinosteroids. Concerning secondary metabolism, PHs induced the accumulation of aliphatic glucosinolates, alkaloids, and phenylpropanoids, potentially eliciting crop resilience to stress conditions. Here, we confirm that PHs may have a hormone-like activity, and that their application can modulate plant growth, likely interfering with signaling processes. Noteworthy, the heterogenicity of the botanical origin supported the distinctive and peculiar metabolomic responses we observed across the products tested. While supporting their biostimulant activity, these findings suggest that a generalized crop response to PHs cannot be defined and that specific effects are rather to be investigated. MDPI 2021-02-08 /pmc/articles/PMC7914860/ /pubmed/33567668 http://dx.doi.org/10.3390/plants10020326 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ceccarelli, Angela Valentina
Miras-Moreno, Begoña
Buffagni, Valentina
Senizza, Biancamaria
Pii, Youry
Cardarelli, Mariateresa
Rouphael, Youssef
Colla, Giuseppe
Lucini, Luigi
Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism
title Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism
title_full Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism
title_fullStr Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism
title_full_unstemmed Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism
title_short Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism
title_sort foliar application of different vegetal-derived protein hydrolysates distinctively modulates tomato root development and metabolism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914860/
https://www.ncbi.nlm.nih.gov/pubmed/33567668
http://dx.doi.org/10.3390/plants10020326
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