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The Arabidopsis TETRATRICOPEPTIDE THIOREDOXIN-LIKE 1 Gene Is Involved in Anisotropic Root Growth during Osmotic Stress Adaptation

Mutations in the Arabidopsis TETRATRICOPEPTIDE THIOREDOXIN-LIKE 1 (TTL1) gene cause reduced tolerance to osmotic stress evidenced by an arrest in root growth and root swelling, which makes it an interesting model to explore how root growth is controlled under stress conditions. We found that osmotic...

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Autores principales: Cuadrado-Pedetti, María Belén, Rauschert, Inés, Sainz, María Martha, Amorim-Silva, Vítor, Botella, Miguel Angel, Borsani, Omar, Sotelo-Silveira, Mariana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915054/
https://www.ncbi.nlm.nih.gov/pubmed/33562207
http://dx.doi.org/10.3390/genes12020236
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author Cuadrado-Pedetti, María Belén
Rauschert, Inés
Sainz, María Martha
Amorim-Silva, Vítor
Botella, Miguel Angel
Borsani, Omar
Sotelo-Silveira, Mariana
author_facet Cuadrado-Pedetti, María Belén
Rauschert, Inés
Sainz, María Martha
Amorim-Silva, Vítor
Botella, Miguel Angel
Borsani, Omar
Sotelo-Silveira, Mariana
author_sort Cuadrado-Pedetti, María Belén
collection PubMed
description Mutations in the Arabidopsis TETRATRICOPEPTIDE THIOREDOXIN-LIKE 1 (TTL1) gene cause reduced tolerance to osmotic stress evidenced by an arrest in root growth and root swelling, which makes it an interesting model to explore how root growth is controlled under stress conditions. We found that osmotic stress reduced the growth rate of the primary root by inhibiting the cell elongation in the elongation zone followed by a reduction in the number of cortical cells in the proximal meristem. We then studied the stiffness of epidermal cell walls in the root elongation zone of ttl1 mutants under osmotic stress using atomic force microscopy. In plants grown in control conditions, the mean apparent elastic modulus was 448% higher for live Col-0 cell walls than for ttl1 (88.1 ± 2.8 vs. 16.08 ± 6.9 kPa). Seven days of osmotic stress caused an increase in the stiffness in the cell wall of the cells from the elongation zone of 87% and 84% for Col-0 and ttl1, respectively. These findings suggest that TTL1 may play a role controlling cell expansion orientation during root growth, necessary for osmotic stress adaptation.
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spelling pubmed-79150542021-03-01 The Arabidopsis TETRATRICOPEPTIDE THIOREDOXIN-LIKE 1 Gene Is Involved in Anisotropic Root Growth during Osmotic Stress Adaptation Cuadrado-Pedetti, María Belén Rauschert, Inés Sainz, María Martha Amorim-Silva, Vítor Botella, Miguel Angel Borsani, Omar Sotelo-Silveira, Mariana Genes (Basel) Article Mutations in the Arabidopsis TETRATRICOPEPTIDE THIOREDOXIN-LIKE 1 (TTL1) gene cause reduced tolerance to osmotic stress evidenced by an arrest in root growth and root swelling, which makes it an interesting model to explore how root growth is controlled under stress conditions. We found that osmotic stress reduced the growth rate of the primary root by inhibiting the cell elongation in the elongation zone followed by a reduction in the number of cortical cells in the proximal meristem. We then studied the stiffness of epidermal cell walls in the root elongation zone of ttl1 mutants under osmotic stress using atomic force microscopy. In plants grown in control conditions, the mean apparent elastic modulus was 448% higher for live Col-0 cell walls than for ttl1 (88.1 ± 2.8 vs. 16.08 ± 6.9 kPa). Seven days of osmotic stress caused an increase in the stiffness in the cell wall of the cells from the elongation zone of 87% and 84% for Col-0 and ttl1, respectively. These findings suggest that TTL1 may play a role controlling cell expansion orientation during root growth, necessary for osmotic stress adaptation. MDPI 2021-02-07 /pmc/articles/PMC7915054/ /pubmed/33562207 http://dx.doi.org/10.3390/genes12020236 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cuadrado-Pedetti, María Belén
Rauschert, Inés
Sainz, María Martha
Amorim-Silva, Vítor
Botella, Miguel Angel
Borsani, Omar
Sotelo-Silveira, Mariana
The Arabidopsis TETRATRICOPEPTIDE THIOREDOXIN-LIKE 1 Gene Is Involved in Anisotropic Root Growth during Osmotic Stress Adaptation
title The Arabidopsis TETRATRICOPEPTIDE THIOREDOXIN-LIKE 1 Gene Is Involved in Anisotropic Root Growth during Osmotic Stress Adaptation
title_full The Arabidopsis TETRATRICOPEPTIDE THIOREDOXIN-LIKE 1 Gene Is Involved in Anisotropic Root Growth during Osmotic Stress Adaptation
title_fullStr The Arabidopsis TETRATRICOPEPTIDE THIOREDOXIN-LIKE 1 Gene Is Involved in Anisotropic Root Growth during Osmotic Stress Adaptation
title_full_unstemmed The Arabidopsis TETRATRICOPEPTIDE THIOREDOXIN-LIKE 1 Gene Is Involved in Anisotropic Root Growth during Osmotic Stress Adaptation
title_short The Arabidopsis TETRATRICOPEPTIDE THIOREDOXIN-LIKE 1 Gene Is Involved in Anisotropic Root Growth during Osmotic Stress Adaptation
title_sort arabidopsis tetratricopeptide thioredoxin-like 1 gene is involved in anisotropic root growth during osmotic stress adaptation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915054/
https://www.ncbi.nlm.nih.gov/pubmed/33562207
http://dx.doi.org/10.3390/genes12020236
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