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Methyl Jasmonate and Methyl-β-Cyclodextrin Individually Boost Triterpenoid Biosynthesis in Chlamydomonas Reinhardtii UVM4

The commercialisation of valuable plant triterpenoids faces major challenges, including low abundance in natural hosts and costly downstream purification procedures. Endeavours to produce these compounds at industrial scale using microbial systems are gaining attention. Here, we report on a strategy...

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Autores principales: Commault, Audrey S., Kuzhiumparambil, Unnikrishnan, Herdean, Andrei, Fabris, Michele, Jaramillo-Madrid, Ana Cristina, Abbriano, Raffaela M., Ralph, Peter J., Pernice, Mathieu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915139/
https://www.ncbi.nlm.nih.gov/pubmed/33562714
http://dx.doi.org/10.3390/ph14020125
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author Commault, Audrey S.
Kuzhiumparambil, Unnikrishnan
Herdean, Andrei
Fabris, Michele
Jaramillo-Madrid, Ana Cristina
Abbriano, Raffaela M.
Ralph, Peter J.
Pernice, Mathieu
author_facet Commault, Audrey S.
Kuzhiumparambil, Unnikrishnan
Herdean, Andrei
Fabris, Michele
Jaramillo-Madrid, Ana Cristina
Abbriano, Raffaela M.
Ralph, Peter J.
Pernice, Mathieu
author_sort Commault, Audrey S.
collection PubMed
description The commercialisation of valuable plant triterpenoids faces major challenges, including low abundance in natural hosts and costly downstream purification procedures. Endeavours to produce these compounds at industrial scale using microbial systems are gaining attention. Here, we report on a strategy to enrich the biomass of the biotechnologically-relevant Chlamydomonas reinhardtii strain UVM4 with valuable triterpenes, such as squalene and (S)-2,3-epoxysqualene. C. reinhardtii UVM4 was subjected to the elicitor compounds methyl jasmonate (MeJA) and methyl-β-cyclodextrine (MβCD) to increase triterpene yields. MeJA treatment triggered oxidative stress, arrested growth, and altered the photosynthetic activity of the cells, while increasing squalene, (S)-2,3-epoxysqualene, and cycloartenol contents. Applying MβCD to cultures of C. reinhardtii lead to the sequestration of the two main sterols (ergosterol and 7-dehydroporiferasterol) into the growth medium and the intracellular accumulation of the intermediate cycloartenol, without compromising cell growth. When MβCD was applied in combination with MeJA, it counteracted the negative effects of MeJA on cell growth and physiology, but no synergistic effect on triterpene yield was observed. Together, our findings provide strategies for the triterpene enrichment of microalgal biomass and medium.
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spelling pubmed-79151392021-03-01 Methyl Jasmonate and Methyl-β-Cyclodextrin Individually Boost Triterpenoid Biosynthesis in Chlamydomonas Reinhardtii UVM4 Commault, Audrey S. Kuzhiumparambil, Unnikrishnan Herdean, Andrei Fabris, Michele Jaramillo-Madrid, Ana Cristina Abbriano, Raffaela M. Ralph, Peter J. Pernice, Mathieu Pharmaceuticals (Basel) Article The commercialisation of valuable plant triterpenoids faces major challenges, including low abundance in natural hosts and costly downstream purification procedures. Endeavours to produce these compounds at industrial scale using microbial systems are gaining attention. Here, we report on a strategy to enrich the biomass of the biotechnologically-relevant Chlamydomonas reinhardtii strain UVM4 with valuable triterpenes, such as squalene and (S)-2,3-epoxysqualene. C. reinhardtii UVM4 was subjected to the elicitor compounds methyl jasmonate (MeJA) and methyl-β-cyclodextrine (MβCD) to increase triterpene yields. MeJA treatment triggered oxidative stress, arrested growth, and altered the photosynthetic activity of the cells, while increasing squalene, (S)-2,3-epoxysqualene, and cycloartenol contents. Applying MβCD to cultures of C. reinhardtii lead to the sequestration of the two main sterols (ergosterol and 7-dehydroporiferasterol) into the growth medium and the intracellular accumulation of the intermediate cycloartenol, without compromising cell growth. When MβCD was applied in combination with MeJA, it counteracted the negative effects of MeJA on cell growth and physiology, but no synergistic effect on triterpene yield was observed. Together, our findings provide strategies for the triterpene enrichment of microalgal biomass and medium. MDPI 2021-02-05 /pmc/articles/PMC7915139/ /pubmed/33562714 http://dx.doi.org/10.3390/ph14020125 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Commault, Audrey S.
Kuzhiumparambil, Unnikrishnan
Herdean, Andrei
Fabris, Michele
Jaramillo-Madrid, Ana Cristina
Abbriano, Raffaela M.
Ralph, Peter J.
Pernice, Mathieu
Methyl Jasmonate and Methyl-β-Cyclodextrin Individually Boost Triterpenoid Biosynthesis in Chlamydomonas Reinhardtii UVM4
title Methyl Jasmonate and Methyl-β-Cyclodextrin Individually Boost Triterpenoid Biosynthesis in Chlamydomonas Reinhardtii UVM4
title_full Methyl Jasmonate and Methyl-β-Cyclodextrin Individually Boost Triterpenoid Biosynthesis in Chlamydomonas Reinhardtii UVM4
title_fullStr Methyl Jasmonate and Methyl-β-Cyclodextrin Individually Boost Triterpenoid Biosynthesis in Chlamydomonas Reinhardtii UVM4
title_full_unstemmed Methyl Jasmonate and Methyl-β-Cyclodextrin Individually Boost Triterpenoid Biosynthesis in Chlamydomonas Reinhardtii UVM4
title_short Methyl Jasmonate and Methyl-β-Cyclodextrin Individually Boost Triterpenoid Biosynthesis in Chlamydomonas Reinhardtii UVM4
title_sort methyl jasmonate and methyl-β-cyclodextrin individually boost triterpenoid biosynthesis in chlamydomonas reinhardtii uvm4
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915139/
https://www.ncbi.nlm.nih.gov/pubmed/33562714
http://dx.doi.org/10.3390/ph14020125
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