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Isolation and Characterization of Feline Wharton’s Jelly-Derived Mesenchymal Stem Cells

Wharton’s jelly is a well-known mesenchymal stem cell source in many species, including humans. However, there have been no reports confirming the presence of mesenchymal stem cells in Wharton’s jelly in cats. The purpose of this study was to isolate mesenchymal stem cells (MSCs) from the Wharton’s...

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Autores principales: Seo, Min-Soo, Kang, Kyung-Ku, Oh, Se-Kyung, Sung, Soo-Eun, Kim, Kil-Soo, Kwon, Young-Sam, Yun, Sungho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915203/
https://www.ncbi.nlm.nih.gov/pubmed/33562192
http://dx.doi.org/10.3390/vetsci8020024
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author Seo, Min-Soo
Kang, Kyung-Ku
Oh, Se-Kyung
Sung, Soo-Eun
Kim, Kil-Soo
Kwon, Young-Sam
Yun, Sungho
author_facet Seo, Min-Soo
Kang, Kyung-Ku
Oh, Se-Kyung
Sung, Soo-Eun
Kim, Kil-Soo
Kwon, Young-Sam
Yun, Sungho
author_sort Seo, Min-Soo
collection PubMed
description Wharton’s jelly is a well-known mesenchymal stem cell source in many species, including humans. However, there have been no reports confirming the presence of mesenchymal stem cells in Wharton’s jelly in cats. The purpose of this study was to isolate mesenchymal stem cells (MSCs) from the Wharton’s jelly of cats and to characterize stem cells. In this study, feline Wharton’s jelly-derived mesenchymal stem cells (fWJ-MSCs) were isolated and successfully cultured. fWJ-MSCs were maintained and the proliferative potential was measured by cumulative population doubling level (CPDL) test, scratch test, and colony forming unit (CFU) test. Stem cell marker, karyotyping and immunophenotyping analysis by flow cytometry showed that fWJ-MSCs possessed characteristic mesenchymal stem cell markers. To confirm the differentiation potential, we performed osteogenic, adipogenic and chondrogenic induction under each differentiation condition. fWJ-MSCs has the ability to differentiate into multiple lineages, including osteogenic, adipogenic and chondrogenic differentiation. This study shows that Wharton’s jelly of cat can be a good source of mesenchymal stem cells. In addition, fWJ-MSCs may be useful for stem cell-based therapeutic applications in feline medicine.
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spelling pubmed-79152032021-03-01 Isolation and Characterization of Feline Wharton’s Jelly-Derived Mesenchymal Stem Cells Seo, Min-Soo Kang, Kyung-Ku Oh, Se-Kyung Sung, Soo-Eun Kim, Kil-Soo Kwon, Young-Sam Yun, Sungho Vet Sci Article Wharton’s jelly is a well-known mesenchymal stem cell source in many species, including humans. However, there have been no reports confirming the presence of mesenchymal stem cells in Wharton’s jelly in cats. The purpose of this study was to isolate mesenchymal stem cells (MSCs) from the Wharton’s jelly of cats and to characterize stem cells. In this study, feline Wharton’s jelly-derived mesenchymal stem cells (fWJ-MSCs) were isolated and successfully cultured. fWJ-MSCs were maintained and the proliferative potential was measured by cumulative population doubling level (CPDL) test, scratch test, and colony forming unit (CFU) test. Stem cell marker, karyotyping and immunophenotyping analysis by flow cytometry showed that fWJ-MSCs possessed characteristic mesenchymal stem cell markers. To confirm the differentiation potential, we performed osteogenic, adipogenic and chondrogenic induction under each differentiation condition. fWJ-MSCs has the ability to differentiate into multiple lineages, including osteogenic, adipogenic and chondrogenic differentiation. This study shows that Wharton’s jelly of cat can be a good source of mesenchymal stem cells. In addition, fWJ-MSCs may be useful for stem cell-based therapeutic applications in feline medicine. MDPI 2021-02-07 /pmc/articles/PMC7915203/ /pubmed/33562192 http://dx.doi.org/10.3390/vetsci8020024 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Seo, Min-Soo
Kang, Kyung-Ku
Oh, Se-Kyung
Sung, Soo-Eun
Kim, Kil-Soo
Kwon, Young-Sam
Yun, Sungho
Isolation and Characterization of Feline Wharton’s Jelly-Derived Mesenchymal Stem Cells
title Isolation and Characterization of Feline Wharton’s Jelly-Derived Mesenchymal Stem Cells
title_full Isolation and Characterization of Feline Wharton’s Jelly-Derived Mesenchymal Stem Cells
title_fullStr Isolation and Characterization of Feline Wharton’s Jelly-Derived Mesenchymal Stem Cells
title_full_unstemmed Isolation and Characterization of Feline Wharton’s Jelly-Derived Mesenchymal Stem Cells
title_short Isolation and Characterization of Feline Wharton’s Jelly-Derived Mesenchymal Stem Cells
title_sort isolation and characterization of feline wharton’s jelly-derived mesenchymal stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915203/
https://www.ncbi.nlm.nih.gov/pubmed/33562192
http://dx.doi.org/10.3390/vetsci8020024
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