To Be There or Not to Be There, That Is the Question—On the Problem of Delayed Sampling of Entomological Evidence

SIMPLE SUMMARY: Proper evidence sampling is at the heart of a sound forensic opinion and failure to follow the standards and guidelines can have serious consequences for the report and expert testimony in court. In casework, forensic entomologists often must base their expert opinion on information about the case and insect evidence provided by third parties, and this presents pitfalls. We analyzed two of those: delayed evidence sampling and the effect of low-temperature storage of the body prior to the autopsy. Our study shows that sampling at the scene is advisable to facilitate a sound entomological report and that the cooling sequence of a corpse must be completely tracked between its removal from the scene until the insect sampling. ABSTRACT: The aim of the current study was to analyze two major pitfalls in forensic entomological casework: delayed evidence sampling and the effect of low-temperature storage of the body. For this purpose, temperature profiles of heavily infested corpses during cooling and cases in which insect evidence was collected both at the scene and during autopsy were evaluated with regard to species composition and development stages found. The results show that the temperature in the body bags remained at higher average temperatures up to 10 °C relative to the mortuary cooler, therefore, sufficient for larval development, with significant differences in temperature between larval aggregations on one and the same body. In addition, we found large differences both in species number, species composition, and the developmental stages found at the scene and during the autopsy. These data and observations underscore the importance of sampling evidence at the scene and recording temperatures throughout the cooling period of a body.