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Increased Tenascin C, Osteopontin and HSP90 Levels in Plasmatic Small Extracellular Vesicles of Pediatric ALK-Positive Anaplastic Large Cell Lymphoma: New Prognostic Biomarkers?

Over the past 15 years, several biological and pathological characteristics proved their significance in pediatric anaplastic lymphoma kinase (ALK)-positive anaplastic large-cell lymphoma (ALCL) prognostic stratification. However, the identification of new non-invasive disease biomarkers, relying on...

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Autores principales: Lovisa, Federica, Garbin, Anna, Crotti, Sara, Di Battista, Piero, Gallingani, Ilaria, Damanti, Carlotta Caterina, Tosato, Anna, Carraro, Elisa, Pillon, Marta, Mafakheri, Erfan, Romanato, Filippo, Gaffo, Enrico, Biffi, Alessandra, Bortoluzzi, Stefania, Agostini, Marco, Mussolin, Lara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915848/
https://www.ncbi.nlm.nih.gov/pubmed/33562105
http://dx.doi.org/10.3390/diagnostics11020253
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author Lovisa, Federica
Garbin, Anna
Crotti, Sara
Di Battista, Piero
Gallingani, Ilaria
Damanti, Carlotta Caterina
Tosato, Anna
Carraro, Elisa
Pillon, Marta
Mafakheri, Erfan
Romanato, Filippo
Gaffo, Enrico
Biffi, Alessandra
Bortoluzzi, Stefania
Agostini, Marco
Mussolin, Lara
author_facet Lovisa, Federica
Garbin, Anna
Crotti, Sara
Di Battista, Piero
Gallingani, Ilaria
Damanti, Carlotta Caterina
Tosato, Anna
Carraro, Elisa
Pillon, Marta
Mafakheri, Erfan
Romanato, Filippo
Gaffo, Enrico
Biffi, Alessandra
Bortoluzzi, Stefania
Agostini, Marco
Mussolin, Lara
author_sort Lovisa, Federica
collection PubMed
description Over the past 15 years, several biological and pathological characteristics proved their significance in pediatric anaplastic lymphoma kinase (ALK)-positive anaplastic large-cell lymphoma (ALCL) prognostic stratification. However, the identification of new non-invasive disease biomarkers, relying on the most important disease mechanisms, is still necessary. In recent years, plasmatic circulating small extracellular vesicles (S-EVs) gathered great importance both as stable biomarker carriers and active players in tumorigenesis. In the present work, we performed a comprehensive study on the proteomic composition of plasmatic S-EVs of pediatric ALCL patients compared to healthy donors (HDs). By using a mass spectrometry-based proteomics approach, we identified 50 proteins significantly overrepresented in S-EVs of ALCL patients. Gene Ontology enrichment analysis disclosed cellular components and molecular functions connected with S-EV origin and vesicular trafficking, whereas cell adhesion, glycosaminoglycan metabolic process, extracellular matrix organization, collagen fibril organization and acute phase response were the most enriched biological processes. Of importance, consistently with the presence of nucleophosmin (NPM)-ALK fusion protein in ALCL cells, a topological enrichment analysis based on Reactome- and Kyoto Encyclopedia of Genes and Genomes (KEGG)-derived networks highlighted a dramatic increase in proteins of the phosphatidylinositol 3-kinase (PI3K)/AKT pathway in ALCL S-EVs, which included heat shock protein 90-kDa isoform alpha 1 (HSP90AA1), osteopontin (SPP1/OPN) and tenascin C (TNC). These results were validated by Western blotting analysis on a panel of ALCL and HD cases. Further research is warranted to better define the role of these S-EV proteins as diagnostic and, possibly, prognostic parameters at diagnosis and for ALCL disease monitoring.
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spelling pubmed-79158482021-03-01 Increased Tenascin C, Osteopontin and HSP90 Levels in Plasmatic Small Extracellular Vesicles of Pediatric ALK-Positive Anaplastic Large Cell Lymphoma: New Prognostic Biomarkers? Lovisa, Federica Garbin, Anna Crotti, Sara Di Battista, Piero Gallingani, Ilaria Damanti, Carlotta Caterina Tosato, Anna Carraro, Elisa Pillon, Marta Mafakheri, Erfan Romanato, Filippo Gaffo, Enrico Biffi, Alessandra Bortoluzzi, Stefania Agostini, Marco Mussolin, Lara Diagnostics (Basel) Article Over the past 15 years, several biological and pathological characteristics proved their significance in pediatric anaplastic lymphoma kinase (ALK)-positive anaplastic large-cell lymphoma (ALCL) prognostic stratification. However, the identification of new non-invasive disease biomarkers, relying on the most important disease mechanisms, is still necessary. In recent years, plasmatic circulating small extracellular vesicles (S-EVs) gathered great importance both as stable biomarker carriers and active players in tumorigenesis. In the present work, we performed a comprehensive study on the proteomic composition of plasmatic S-EVs of pediatric ALCL patients compared to healthy donors (HDs). By using a mass spectrometry-based proteomics approach, we identified 50 proteins significantly overrepresented in S-EVs of ALCL patients. Gene Ontology enrichment analysis disclosed cellular components and molecular functions connected with S-EV origin and vesicular trafficking, whereas cell adhesion, glycosaminoglycan metabolic process, extracellular matrix organization, collagen fibril organization and acute phase response were the most enriched biological processes. Of importance, consistently with the presence of nucleophosmin (NPM)-ALK fusion protein in ALCL cells, a topological enrichment analysis based on Reactome- and Kyoto Encyclopedia of Genes and Genomes (KEGG)-derived networks highlighted a dramatic increase in proteins of the phosphatidylinositol 3-kinase (PI3K)/AKT pathway in ALCL S-EVs, which included heat shock protein 90-kDa isoform alpha 1 (HSP90AA1), osteopontin (SPP1/OPN) and tenascin C (TNC). These results were validated by Western blotting analysis on a panel of ALCL and HD cases. Further research is warranted to better define the role of these S-EV proteins as diagnostic and, possibly, prognostic parameters at diagnosis and for ALCL disease monitoring. MDPI 2021-02-06 /pmc/articles/PMC7915848/ /pubmed/33562105 http://dx.doi.org/10.3390/diagnostics11020253 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lovisa, Federica
Garbin, Anna
Crotti, Sara
Di Battista, Piero
Gallingani, Ilaria
Damanti, Carlotta Caterina
Tosato, Anna
Carraro, Elisa
Pillon, Marta
Mafakheri, Erfan
Romanato, Filippo
Gaffo, Enrico
Biffi, Alessandra
Bortoluzzi, Stefania
Agostini, Marco
Mussolin, Lara
Increased Tenascin C, Osteopontin and HSP90 Levels in Plasmatic Small Extracellular Vesicles of Pediatric ALK-Positive Anaplastic Large Cell Lymphoma: New Prognostic Biomarkers?
title Increased Tenascin C, Osteopontin and HSP90 Levels in Plasmatic Small Extracellular Vesicles of Pediatric ALK-Positive Anaplastic Large Cell Lymphoma: New Prognostic Biomarkers?
title_full Increased Tenascin C, Osteopontin and HSP90 Levels in Plasmatic Small Extracellular Vesicles of Pediatric ALK-Positive Anaplastic Large Cell Lymphoma: New Prognostic Biomarkers?
title_fullStr Increased Tenascin C, Osteopontin and HSP90 Levels in Plasmatic Small Extracellular Vesicles of Pediatric ALK-Positive Anaplastic Large Cell Lymphoma: New Prognostic Biomarkers?
title_full_unstemmed Increased Tenascin C, Osteopontin and HSP90 Levels in Plasmatic Small Extracellular Vesicles of Pediatric ALK-Positive Anaplastic Large Cell Lymphoma: New Prognostic Biomarkers?
title_short Increased Tenascin C, Osteopontin and HSP90 Levels in Plasmatic Small Extracellular Vesicles of Pediatric ALK-Positive Anaplastic Large Cell Lymphoma: New Prognostic Biomarkers?
title_sort increased tenascin c, osteopontin and hsp90 levels in plasmatic small extracellular vesicles of pediatric alk-positive anaplastic large cell lymphoma: new prognostic biomarkers?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915848/
https://www.ncbi.nlm.nih.gov/pubmed/33562105
http://dx.doi.org/10.3390/diagnostics11020253
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