Cargando…

Comparative Mapping of the Macrochromosomes of Eight Avian Species Provides Further Insight into Their Phylogenetic Relationships and Avian Karyotype Evolution

Avian genomes typically consist of ~10 pairs of macro- and ~30 pairs of microchromosomes. While inter-chromosomally, a pattern emerges of very little change (with notable exceptions) throughout evolution, intrachromosomal changes remain relatively poorly studied. To rectify this, here we use a pan-a...

Descripción completa

Detalles Bibliográficos
Autores principales: Kiazim, Lucas G., O’Connor, Rebecca E., Larkin, Denis M., Romanov, Michael N., Narushin, Valery G., Brazhnik, Evgeni A., Griffin, Darren K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7916199/
https://www.ncbi.nlm.nih.gov/pubmed/33572408
http://dx.doi.org/10.3390/cells10020362
_version_ 1783657424783147008
author Kiazim, Lucas G.
O’Connor, Rebecca E.
Larkin, Denis M.
Romanov, Michael N.
Narushin, Valery G.
Brazhnik, Evgeni A.
Griffin, Darren K.
author_facet Kiazim, Lucas G.
O’Connor, Rebecca E.
Larkin, Denis M.
Romanov, Michael N.
Narushin, Valery G.
Brazhnik, Evgeni A.
Griffin, Darren K.
author_sort Kiazim, Lucas G.
collection PubMed
description Avian genomes typically consist of ~10 pairs of macro- and ~30 pairs of microchromosomes. While inter-chromosomally, a pattern emerges of very little change (with notable exceptions) throughout evolution, intrachromosomal changes remain relatively poorly studied. To rectify this, here we use a pan-avian universally hybridising set of 74 chicken bacterial artificial chromosome (BAC) probes on the macrochromosomes of eight bird species: common blackbird, Atlantic canary, Eurasian woodcock, helmeted guinea fowl, houbara bustard, mallard duck, and rock dove. A combination of molecular cytogenetic, bioinformatics, and mathematical analyses allowed the building of comparative cytogenetic maps, reconstruction of a putative Neognathae ancestor, and assessment of chromosome rearrangement patterns and phylogenetic relationships in the studied neognath lineages. We observe that, as with our previous studies, chicken appears to have the karyotype most similar to the ancestor; however, previous reports of an increased rate of intrachromosomal change in Passeriformes (songbirds) appear not to be the case in our dataset. The use of this universally hybridizing probe set is applicable not only for the re-tracing of avian karyotype evolution but, potentially, for reconstructing genome assemblies.
format Online
Article
Text
id pubmed-7916199
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79161992021-03-01 Comparative Mapping of the Macrochromosomes of Eight Avian Species Provides Further Insight into Their Phylogenetic Relationships and Avian Karyotype Evolution Kiazim, Lucas G. O’Connor, Rebecca E. Larkin, Denis M. Romanov, Michael N. Narushin, Valery G. Brazhnik, Evgeni A. Griffin, Darren K. Cells Article Avian genomes typically consist of ~10 pairs of macro- and ~30 pairs of microchromosomes. While inter-chromosomally, a pattern emerges of very little change (with notable exceptions) throughout evolution, intrachromosomal changes remain relatively poorly studied. To rectify this, here we use a pan-avian universally hybridising set of 74 chicken bacterial artificial chromosome (BAC) probes on the macrochromosomes of eight bird species: common blackbird, Atlantic canary, Eurasian woodcock, helmeted guinea fowl, houbara bustard, mallard duck, and rock dove. A combination of molecular cytogenetic, bioinformatics, and mathematical analyses allowed the building of comparative cytogenetic maps, reconstruction of a putative Neognathae ancestor, and assessment of chromosome rearrangement patterns and phylogenetic relationships in the studied neognath lineages. We observe that, as with our previous studies, chicken appears to have the karyotype most similar to the ancestor; however, previous reports of an increased rate of intrachromosomal change in Passeriformes (songbirds) appear not to be the case in our dataset. The use of this universally hybridizing probe set is applicable not only for the re-tracing of avian karyotype evolution but, potentially, for reconstructing genome assemblies. MDPI 2021-02-09 /pmc/articles/PMC7916199/ /pubmed/33572408 http://dx.doi.org/10.3390/cells10020362 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kiazim, Lucas G.
O’Connor, Rebecca E.
Larkin, Denis M.
Romanov, Michael N.
Narushin, Valery G.
Brazhnik, Evgeni A.
Griffin, Darren K.
Comparative Mapping of the Macrochromosomes of Eight Avian Species Provides Further Insight into Their Phylogenetic Relationships and Avian Karyotype Evolution
title Comparative Mapping of the Macrochromosomes of Eight Avian Species Provides Further Insight into Their Phylogenetic Relationships and Avian Karyotype Evolution
title_full Comparative Mapping of the Macrochromosomes of Eight Avian Species Provides Further Insight into Their Phylogenetic Relationships and Avian Karyotype Evolution
title_fullStr Comparative Mapping of the Macrochromosomes of Eight Avian Species Provides Further Insight into Their Phylogenetic Relationships and Avian Karyotype Evolution
title_full_unstemmed Comparative Mapping of the Macrochromosomes of Eight Avian Species Provides Further Insight into Their Phylogenetic Relationships and Avian Karyotype Evolution
title_short Comparative Mapping of the Macrochromosomes of Eight Avian Species Provides Further Insight into Their Phylogenetic Relationships and Avian Karyotype Evolution
title_sort comparative mapping of the macrochromosomes of eight avian species provides further insight into their phylogenetic relationships and avian karyotype evolution
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7916199/
https://www.ncbi.nlm.nih.gov/pubmed/33572408
http://dx.doi.org/10.3390/cells10020362
work_keys_str_mv AT kiazimlucasg comparativemappingofthemacrochromosomesofeightavianspeciesprovidesfurtherinsightintotheirphylogeneticrelationshipsandaviankaryotypeevolution
AT oconnorrebeccae comparativemappingofthemacrochromosomesofeightavianspeciesprovidesfurtherinsightintotheirphylogeneticrelationshipsandaviankaryotypeevolution
AT larkindenism comparativemappingofthemacrochromosomesofeightavianspeciesprovidesfurtherinsightintotheirphylogeneticrelationshipsandaviankaryotypeevolution
AT romanovmichaeln comparativemappingofthemacrochromosomesofeightavianspeciesprovidesfurtherinsightintotheirphylogeneticrelationshipsandaviankaryotypeevolution
AT narushinvaleryg comparativemappingofthemacrochromosomesofeightavianspeciesprovidesfurtherinsightintotheirphylogeneticrelationshipsandaviankaryotypeevolution
AT brazhnikevgenia comparativemappingofthemacrochromosomesofeightavianspeciesprovidesfurtherinsightintotheirphylogeneticrelationshipsandaviankaryotypeevolution
AT griffindarrenk comparativemappingofthemacrochromosomesofeightavianspeciesprovidesfurtherinsightintotheirphylogeneticrelationshipsandaviankaryotypeevolution