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Transcriptome Response of Liver and Muscle in Heat-Stressed Laying Hens

Exposure to high ambient temperature has detrimental effects on poultry welfare and production. Although changes in gene expression due to heat exposure have been well described for broiler chickens, knowledge of the effects of heat on laying hens is still relatively limited. In this study, we profi...

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Autores principales: Wang, Yan, Jia, Xinzheng, Hsieh, John C. F., Monson, Melissa S., Zhang, Jibin, Shu, Dingming, Nie, Qinghua, Persia, Michael E., Rothschild, Max F., Lamont, Susan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7916550/
https://www.ncbi.nlm.nih.gov/pubmed/33578825
http://dx.doi.org/10.3390/genes12020255
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author Wang, Yan
Jia, Xinzheng
Hsieh, John C. F.
Monson, Melissa S.
Zhang, Jibin
Shu, Dingming
Nie, Qinghua
Persia, Michael E.
Rothschild, Max F.
Lamont, Susan J.
author_facet Wang, Yan
Jia, Xinzheng
Hsieh, John C. F.
Monson, Melissa S.
Zhang, Jibin
Shu, Dingming
Nie, Qinghua
Persia, Michael E.
Rothschild, Max F.
Lamont, Susan J.
author_sort Wang, Yan
collection PubMed
description Exposure to high ambient temperature has detrimental effects on poultry welfare and production. Although changes in gene expression due to heat exposure have been well described for broiler chickens, knowledge of the effects of heat on laying hens is still relatively limited. In this study, we profiled the transcriptome for pectoralis major muscle (n = 24) and liver (n = 24), during a 4-week cyclic heating experiment performed on layers in the early phase of egg production. Both heat-control and time-based contrasts were analyzed to determine differentially expressed genes (DEGs). Heat exposure induced different changes in gene expression for the two tissues, and we also observed changes in gene expression over time in the control animals suggesting that metabolic changes occurred during the transition from onset of lay to peak egg production. A total of 73 DEGs in liver were shared between the 3 h heat-control contrast, and the 4-week versus 3 h time contrast in the control group, suggesting a core set of genes that is responsible for maintenance of metabolic homeostasis regardless of the physiologic stressor (heat or commencing egg production). The identified DEGs improve our understanding of the layer’s response to stressors and may serve as targets for genetic selection in the future to improve resilience.
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spelling pubmed-79165502021-03-01 Transcriptome Response of Liver and Muscle in Heat-Stressed Laying Hens Wang, Yan Jia, Xinzheng Hsieh, John C. F. Monson, Melissa S. Zhang, Jibin Shu, Dingming Nie, Qinghua Persia, Michael E. Rothschild, Max F. Lamont, Susan J. Genes (Basel) Article Exposure to high ambient temperature has detrimental effects on poultry welfare and production. Although changes in gene expression due to heat exposure have been well described for broiler chickens, knowledge of the effects of heat on laying hens is still relatively limited. In this study, we profiled the transcriptome for pectoralis major muscle (n = 24) and liver (n = 24), during a 4-week cyclic heating experiment performed on layers in the early phase of egg production. Both heat-control and time-based contrasts were analyzed to determine differentially expressed genes (DEGs). Heat exposure induced different changes in gene expression for the two tissues, and we also observed changes in gene expression over time in the control animals suggesting that metabolic changes occurred during the transition from onset of lay to peak egg production. A total of 73 DEGs in liver were shared between the 3 h heat-control contrast, and the 4-week versus 3 h time contrast in the control group, suggesting a core set of genes that is responsible for maintenance of metabolic homeostasis regardless of the physiologic stressor (heat or commencing egg production). The identified DEGs improve our understanding of the layer’s response to stressors and may serve as targets for genetic selection in the future to improve resilience. MDPI 2021-02-10 /pmc/articles/PMC7916550/ /pubmed/33578825 http://dx.doi.org/10.3390/genes12020255 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Yan
Jia, Xinzheng
Hsieh, John C. F.
Monson, Melissa S.
Zhang, Jibin
Shu, Dingming
Nie, Qinghua
Persia, Michael E.
Rothschild, Max F.
Lamont, Susan J.
Transcriptome Response of Liver and Muscle in Heat-Stressed Laying Hens
title Transcriptome Response of Liver and Muscle in Heat-Stressed Laying Hens
title_full Transcriptome Response of Liver and Muscle in Heat-Stressed Laying Hens
title_fullStr Transcriptome Response of Liver and Muscle in Heat-Stressed Laying Hens
title_full_unstemmed Transcriptome Response of Liver and Muscle in Heat-Stressed Laying Hens
title_short Transcriptome Response of Liver and Muscle in Heat-Stressed Laying Hens
title_sort transcriptome response of liver and muscle in heat-stressed laying hens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7916550/
https://www.ncbi.nlm.nih.gov/pubmed/33578825
http://dx.doi.org/10.3390/genes12020255
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