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Green Synthesis of Silver Nanoparticles by Cytobacillus firmus Isolated from the Stem Bark of Terminalia arjuna and Their Antimicrobial Activity

This work reports an eco-friendly synthesis of silver nanoparticles (AgNPs) using endophytic bacteria, Cytobacillus firmus isolated from the stem bark of Terminalia arjuna. The synthesis of AgNPs was confirmed by visual observation as a change in color of the bacterial solution impregnated with silv...

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Autores principales: Sudarsan, Sujesh, Kumar Shankar, Madan, Kumar Belagal Motatis, Anil, Shankar, Sushmitha, Krishnappa, Darshan, Mohan, Chakrabhavi Dhananjaya, Rangappa, Kanchugarakoppal S., Gupta, Vijai Kumar, Siddaiah, Chandra Nayaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7916701/
https://www.ncbi.nlm.nih.gov/pubmed/33578957
http://dx.doi.org/10.3390/biom11020259
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author Sudarsan, Sujesh
Kumar Shankar, Madan
Kumar Belagal Motatis, Anil
Shankar, Sushmitha
Krishnappa, Darshan
Mohan, Chakrabhavi Dhananjaya
Rangappa, Kanchugarakoppal S.
Gupta, Vijai Kumar
Siddaiah, Chandra Nayaka
author_facet Sudarsan, Sujesh
Kumar Shankar, Madan
Kumar Belagal Motatis, Anil
Shankar, Sushmitha
Krishnappa, Darshan
Mohan, Chakrabhavi Dhananjaya
Rangappa, Kanchugarakoppal S.
Gupta, Vijai Kumar
Siddaiah, Chandra Nayaka
author_sort Sudarsan, Sujesh
collection PubMed
description This work reports an eco-friendly synthesis of silver nanoparticles (AgNPs) using endophytic bacteria, Cytobacillus firmus isolated from the stem bark of Terminalia arjuna. The synthesis of AgNPs was confirmed by visual observation as a change in color of the bacterial solution impregnated with silver. Further, the morphology of the AgNPs, average size, and presence of elemental silver were characterized by UV–Visible spectroscopy, scanning electron microscopy, and dynamic light scattering spectroscopy. The roles of endophytic secondary metabolites in the metal reduction, stabilization, and capping of silver nanoparticles were studied by qualitative FTIR spectral peaks. The antimicrobial ability of AgNPs was evaluated against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria and pearl millet blast disease-causing fungi (Magnoporthe grisea). The biosynthesized AgNPs showed good antibacterial and antifungal activities. AgNPs effectively inhibited the bacterial growth in a dose-dependent manner and presented as good antifungal agents towards the growth of Magnoporthe grisea.
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spelling pubmed-79167012021-03-01 Green Synthesis of Silver Nanoparticles by Cytobacillus firmus Isolated from the Stem Bark of Terminalia arjuna and Their Antimicrobial Activity Sudarsan, Sujesh Kumar Shankar, Madan Kumar Belagal Motatis, Anil Shankar, Sushmitha Krishnappa, Darshan Mohan, Chakrabhavi Dhananjaya Rangappa, Kanchugarakoppal S. Gupta, Vijai Kumar Siddaiah, Chandra Nayaka Biomolecules Article This work reports an eco-friendly synthesis of silver nanoparticles (AgNPs) using endophytic bacteria, Cytobacillus firmus isolated from the stem bark of Terminalia arjuna. The synthesis of AgNPs was confirmed by visual observation as a change in color of the bacterial solution impregnated with silver. Further, the morphology of the AgNPs, average size, and presence of elemental silver were characterized by UV–Visible spectroscopy, scanning electron microscopy, and dynamic light scattering spectroscopy. The roles of endophytic secondary metabolites in the metal reduction, stabilization, and capping of silver nanoparticles were studied by qualitative FTIR spectral peaks. The antimicrobial ability of AgNPs was evaluated against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria and pearl millet blast disease-causing fungi (Magnoporthe grisea). The biosynthesized AgNPs showed good antibacterial and antifungal activities. AgNPs effectively inhibited the bacterial growth in a dose-dependent manner and presented as good antifungal agents towards the growth of Magnoporthe grisea. MDPI 2021-02-10 /pmc/articles/PMC7916701/ /pubmed/33578957 http://dx.doi.org/10.3390/biom11020259 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sudarsan, Sujesh
Kumar Shankar, Madan
Kumar Belagal Motatis, Anil
Shankar, Sushmitha
Krishnappa, Darshan
Mohan, Chakrabhavi Dhananjaya
Rangappa, Kanchugarakoppal S.
Gupta, Vijai Kumar
Siddaiah, Chandra Nayaka
Green Synthesis of Silver Nanoparticles by Cytobacillus firmus Isolated from the Stem Bark of Terminalia arjuna and Their Antimicrobial Activity
title Green Synthesis of Silver Nanoparticles by Cytobacillus firmus Isolated from the Stem Bark of Terminalia arjuna and Their Antimicrobial Activity
title_full Green Synthesis of Silver Nanoparticles by Cytobacillus firmus Isolated from the Stem Bark of Terminalia arjuna and Their Antimicrobial Activity
title_fullStr Green Synthesis of Silver Nanoparticles by Cytobacillus firmus Isolated from the Stem Bark of Terminalia arjuna and Their Antimicrobial Activity
title_full_unstemmed Green Synthesis of Silver Nanoparticles by Cytobacillus firmus Isolated from the Stem Bark of Terminalia arjuna and Their Antimicrobial Activity
title_short Green Synthesis of Silver Nanoparticles by Cytobacillus firmus Isolated from the Stem Bark of Terminalia arjuna and Their Antimicrobial Activity
title_sort green synthesis of silver nanoparticles by cytobacillus firmus isolated from the stem bark of terminalia arjuna and their antimicrobial activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7916701/
https://www.ncbi.nlm.nih.gov/pubmed/33578957
http://dx.doi.org/10.3390/biom11020259
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