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Curdlan–Chitosan Electrospun Fibers as Potential Scaffolds for Bone Regeneration

Polysaccharides have received a lot of attention in biomedical research for their high potential as scaffolds owing to their unique biological properties. Fibrillar scaffolds made of chitosan demonstrated high promise in tissue engineering, especially for skin. As far as bone regeneration is concern...

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Autores principales: Toullec, Clément, Le Bideau, Jean, Geoffroy, Valerie, Halgand, Boris, Buchtova, Nela, Molina-Peña, Rodolfo, Garcion, Emmanuel, Avril, Sylvie, Sindji, Laurence, Dube, Admire, Boury, Frank, Jérôme, Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7916722/
https://www.ncbi.nlm.nih.gov/pubmed/33578913
http://dx.doi.org/10.3390/polym13040526
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author Toullec, Clément
Le Bideau, Jean
Geoffroy, Valerie
Halgand, Boris
Buchtova, Nela
Molina-Peña, Rodolfo
Garcion, Emmanuel
Avril, Sylvie
Sindji, Laurence
Dube, Admire
Boury, Frank
Jérôme, Christine
author_facet Toullec, Clément
Le Bideau, Jean
Geoffroy, Valerie
Halgand, Boris
Buchtova, Nela
Molina-Peña, Rodolfo
Garcion, Emmanuel
Avril, Sylvie
Sindji, Laurence
Dube, Admire
Boury, Frank
Jérôme, Christine
author_sort Toullec, Clément
collection PubMed
description Polysaccharides have received a lot of attention in biomedical research for their high potential as scaffolds owing to their unique biological properties. Fibrillar scaffolds made of chitosan demonstrated high promise in tissue engineering, especially for skin. As far as bone regeneration is concerned, curdlan (1,3-β-glucan) is particularly interesting as it enhances bone growth by helping mesenchymal stem cell adhesion, by favoring their differentiation into osteoblasts and by limiting the osteoclastic activity. Therefore, we aim to combine both chitosan and curdlan polysaccharides in a new scaffold for bone regeneration. For that purpose, curdlan was electrospun as a blend with chitosan into a fibrillar scaffold. We show that this novel scaffold is biodegradable (8% at two weeks), exhibits a good swelling behavior (350%) and is non-cytotoxic in vitro. In addition, the benefit of incorporating curdlan in the scaffold was demonstrated in a scratch assay that evidences the ability of curdlan to express its immunomodulatory properties by enhancing cell migration. Thus, these innovative electrospun curdlan–chitosan scaffolds show great potential for bone tissue engineering.
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spelling pubmed-79167222021-03-01 Curdlan–Chitosan Electrospun Fibers as Potential Scaffolds for Bone Regeneration Toullec, Clément Le Bideau, Jean Geoffroy, Valerie Halgand, Boris Buchtova, Nela Molina-Peña, Rodolfo Garcion, Emmanuel Avril, Sylvie Sindji, Laurence Dube, Admire Boury, Frank Jérôme, Christine Polymers (Basel) Article Polysaccharides have received a lot of attention in biomedical research for their high potential as scaffolds owing to their unique biological properties. Fibrillar scaffolds made of chitosan demonstrated high promise in tissue engineering, especially for skin. As far as bone regeneration is concerned, curdlan (1,3-β-glucan) is particularly interesting as it enhances bone growth by helping mesenchymal stem cell adhesion, by favoring their differentiation into osteoblasts and by limiting the osteoclastic activity. Therefore, we aim to combine both chitosan and curdlan polysaccharides in a new scaffold for bone regeneration. For that purpose, curdlan was electrospun as a blend with chitosan into a fibrillar scaffold. We show that this novel scaffold is biodegradable (8% at two weeks), exhibits a good swelling behavior (350%) and is non-cytotoxic in vitro. In addition, the benefit of incorporating curdlan in the scaffold was demonstrated in a scratch assay that evidences the ability of curdlan to express its immunomodulatory properties by enhancing cell migration. Thus, these innovative electrospun curdlan–chitosan scaffolds show great potential for bone tissue engineering. MDPI 2021-02-10 /pmc/articles/PMC7916722/ /pubmed/33578913 http://dx.doi.org/10.3390/polym13040526 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Toullec, Clément
Le Bideau, Jean
Geoffroy, Valerie
Halgand, Boris
Buchtova, Nela
Molina-Peña, Rodolfo
Garcion, Emmanuel
Avril, Sylvie
Sindji, Laurence
Dube, Admire
Boury, Frank
Jérôme, Christine
Curdlan–Chitosan Electrospun Fibers as Potential Scaffolds for Bone Regeneration
title Curdlan–Chitosan Electrospun Fibers as Potential Scaffolds for Bone Regeneration
title_full Curdlan–Chitosan Electrospun Fibers as Potential Scaffolds for Bone Regeneration
title_fullStr Curdlan–Chitosan Electrospun Fibers as Potential Scaffolds for Bone Regeneration
title_full_unstemmed Curdlan–Chitosan Electrospun Fibers as Potential Scaffolds for Bone Regeneration
title_short Curdlan–Chitosan Electrospun Fibers as Potential Scaffolds for Bone Regeneration
title_sort curdlan–chitosan electrospun fibers as potential scaffolds for bone regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7916722/
https://www.ncbi.nlm.nih.gov/pubmed/33578913
http://dx.doi.org/10.3390/polym13040526
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