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Measurement of the Intestinal pH in Mice under Various Conditions Reveals Alkalization Induced by Antibiotics

The intestinal pH can greatly influence the stability and absorption of oral drugs. Therefore, knowledge of intestinal pH is necessary to understand the conditions for drug delivery. This has previously been measured in humans and rats. However, information on intestinal pH in mice is insufficient d...

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Autores principales: Shimizu, Kouki, Seiki, Issei, Goto, Yoshiyuki, Murata, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7916911/
https://www.ncbi.nlm.nih.gov/pubmed/33670214
http://dx.doi.org/10.3390/antibiotics10020180
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author Shimizu, Kouki
Seiki, Issei
Goto, Yoshiyuki
Murata, Takeshi
author_facet Shimizu, Kouki
Seiki, Issei
Goto, Yoshiyuki
Murata, Takeshi
author_sort Shimizu, Kouki
collection PubMed
description The intestinal pH can greatly influence the stability and absorption of oral drugs. Therefore, knowledge of intestinal pH is necessary to understand the conditions for drug delivery. This has previously been measured in humans and rats. However, information on intestinal pH in mice is insufficient despite these animals being used often in preclinical testing. In this study, 72 female ICR mice housed in SPF (specific pathogen-free) conditions were separated into nine groups to determine the intestinal pH under conditions that might cause pH fluctuations, including high-protein diet, ageing, proton pump inhibitor (PPI) treatment, several antibiotic treatment regimens and germ-free mice. pH was measured in samples collected from the ileum, cecum and colon, and compared to control animals. An electrode, 3 mm in diameter, enabled accurate pH measurements with a small amount of gastrointestinal content. Consequently, the pH values in the cecum and colon were increased by high-protein diet, and the pH in the ileum was decreased by PPI. Drastic alkalization was induced by antibiotics, especially in the cecum and colon. The alkalized pH values in germ-free mice suggested that the reduction in the intestinal bacteria caused by antibiotics led to alkalization. Alkalization of the intestinal pH caused by antibiotic treatment was verified in mice. We need further investigations in clinical settings to check whether the same phenomena occur in patients.
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spelling pubmed-79169112021-03-01 Measurement of the Intestinal pH in Mice under Various Conditions Reveals Alkalization Induced by Antibiotics Shimizu, Kouki Seiki, Issei Goto, Yoshiyuki Murata, Takeshi Antibiotics (Basel) Article The intestinal pH can greatly influence the stability and absorption of oral drugs. Therefore, knowledge of intestinal pH is necessary to understand the conditions for drug delivery. This has previously been measured in humans and rats. However, information on intestinal pH in mice is insufficient despite these animals being used often in preclinical testing. In this study, 72 female ICR mice housed in SPF (specific pathogen-free) conditions were separated into nine groups to determine the intestinal pH under conditions that might cause pH fluctuations, including high-protein diet, ageing, proton pump inhibitor (PPI) treatment, several antibiotic treatment regimens and germ-free mice. pH was measured in samples collected from the ileum, cecum and colon, and compared to control animals. An electrode, 3 mm in diameter, enabled accurate pH measurements with a small amount of gastrointestinal content. Consequently, the pH values in the cecum and colon were increased by high-protein diet, and the pH in the ileum was decreased by PPI. Drastic alkalization was induced by antibiotics, especially in the cecum and colon. The alkalized pH values in germ-free mice suggested that the reduction in the intestinal bacteria caused by antibiotics led to alkalization. Alkalization of the intestinal pH caused by antibiotic treatment was verified in mice. We need further investigations in clinical settings to check whether the same phenomena occur in patients. MDPI 2021-02-11 /pmc/articles/PMC7916911/ /pubmed/33670214 http://dx.doi.org/10.3390/antibiotics10020180 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shimizu, Kouki
Seiki, Issei
Goto, Yoshiyuki
Murata, Takeshi
Measurement of the Intestinal pH in Mice under Various Conditions Reveals Alkalization Induced by Antibiotics
title Measurement of the Intestinal pH in Mice under Various Conditions Reveals Alkalization Induced by Antibiotics
title_full Measurement of the Intestinal pH in Mice under Various Conditions Reveals Alkalization Induced by Antibiotics
title_fullStr Measurement of the Intestinal pH in Mice under Various Conditions Reveals Alkalization Induced by Antibiotics
title_full_unstemmed Measurement of the Intestinal pH in Mice under Various Conditions Reveals Alkalization Induced by Antibiotics
title_short Measurement of the Intestinal pH in Mice under Various Conditions Reveals Alkalization Induced by Antibiotics
title_sort measurement of the intestinal ph in mice under various conditions reveals alkalization induced by antibiotics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7916911/
https://www.ncbi.nlm.nih.gov/pubmed/33670214
http://dx.doi.org/10.3390/antibiotics10020180
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