Cargando…

Preparation of Electrospun Small Intestinal Submucosa/Poly(caprolactone-co-Lactide-co-glycolide) Nanofiber Sheet as a Potential Drug Carrier

In this work, we chose small intestine submucosa (SIS) as a drug carrier because SIS possesses good biocompatibility, non-immunogenic property and bio-resorbability, and performed electrospinning for preparation of nanofiber sheets (NS). For the preparation of drug-loaded electrospun SIS nanofiber s...

Descripción completa

Detalles Bibliográficos
Autores principales: Thao, Nguyen Thi Thu, Lee, Surha, Shin, Gi Ru, Kang, Youngji, Choi, Sangdun, Kim, Moon Suk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917610/
https://www.ncbi.nlm.nih.gov/pubmed/33670418
http://dx.doi.org/10.3390/pharmaceutics13020253
_version_ 1783657736666349568
author Thao, Nguyen Thi Thu
Lee, Surha
Shin, Gi Ru
Kang, Youngji
Choi, Sangdun
Kim, Moon Suk
author_facet Thao, Nguyen Thi Thu
Lee, Surha
Shin, Gi Ru
Kang, Youngji
Choi, Sangdun
Kim, Moon Suk
author_sort Thao, Nguyen Thi Thu
collection PubMed
description In this work, we chose small intestine submucosa (SIS) as a drug carrier because SIS possesses good biocompatibility, non-immunogenic property and bio-resorbability, and performed electrospinning for preparation of nanofiber sheets (NS). For the preparation of drug-loaded electrospun SIS nanofiber sheets as a drug carrier, we used poly(ε-caprolactone-ran-l-lactide) (PCLA) copolymers to improve the electrospinning performance of SIS. The electrospinning of SIS and PCLA provided the electrospun SIS/PCLA (S/P)-nanofiber sheet (S/P-NS) with adjustable thickness and areas. The electrospun S/P-NS showed different porosities, pore sizes, diameters and tensile strengths depending on the ratios between SIS and PCLA. The electrospun S/P-NS was used as a drug carrier of the dexamethasone (Dex) and silver sulfadiazine (AgS) drug related to anti-inflammation. Dex-loaded S/P-NS and AgS-loaded S/P-NS was successfully fabricated by the electrospinning. In the in vitro and in vivo release, we successfully confirmed the possibility for the sustained release of Dex and AgS from the Dex-S/P-NS and AgS-S/P-NS for three weeks. In addition, the sustained Dex and AgS release suppressed the macrophage infiltration. Collectively, we achieved feasible development of SIS nanofiber sheets for a sustained Dex and AgS delivery system.
format Online
Article
Text
id pubmed-7917610
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79176102021-03-02 Preparation of Electrospun Small Intestinal Submucosa/Poly(caprolactone-co-Lactide-co-glycolide) Nanofiber Sheet as a Potential Drug Carrier Thao, Nguyen Thi Thu Lee, Surha Shin, Gi Ru Kang, Youngji Choi, Sangdun Kim, Moon Suk Pharmaceutics Article In this work, we chose small intestine submucosa (SIS) as a drug carrier because SIS possesses good biocompatibility, non-immunogenic property and bio-resorbability, and performed electrospinning for preparation of nanofiber sheets (NS). For the preparation of drug-loaded electrospun SIS nanofiber sheets as a drug carrier, we used poly(ε-caprolactone-ran-l-lactide) (PCLA) copolymers to improve the electrospinning performance of SIS. The electrospinning of SIS and PCLA provided the electrospun SIS/PCLA (S/P)-nanofiber sheet (S/P-NS) with adjustable thickness and areas. The electrospun S/P-NS showed different porosities, pore sizes, diameters and tensile strengths depending on the ratios between SIS and PCLA. The electrospun S/P-NS was used as a drug carrier of the dexamethasone (Dex) and silver sulfadiazine (AgS) drug related to anti-inflammation. Dex-loaded S/P-NS and AgS-loaded S/P-NS was successfully fabricated by the electrospinning. In the in vitro and in vivo release, we successfully confirmed the possibility for the sustained release of Dex and AgS from the Dex-S/P-NS and AgS-S/P-NS for three weeks. In addition, the sustained Dex and AgS release suppressed the macrophage infiltration. Collectively, we achieved feasible development of SIS nanofiber sheets for a sustained Dex and AgS delivery system. MDPI 2021-02-11 /pmc/articles/PMC7917610/ /pubmed/33670418 http://dx.doi.org/10.3390/pharmaceutics13020253 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Thao, Nguyen Thi Thu
Lee, Surha
Shin, Gi Ru
Kang, Youngji
Choi, Sangdun
Kim, Moon Suk
Preparation of Electrospun Small Intestinal Submucosa/Poly(caprolactone-co-Lactide-co-glycolide) Nanofiber Sheet as a Potential Drug Carrier
title Preparation of Electrospun Small Intestinal Submucosa/Poly(caprolactone-co-Lactide-co-glycolide) Nanofiber Sheet as a Potential Drug Carrier
title_full Preparation of Electrospun Small Intestinal Submucosa/Poly(caprolactone-co-Lactide-co-glycolide) Nanofiber Sheet as a Potential Drug Carrier
title_fullStr Preparation of Electrospun Small Intestinal Submucosa/Poly(caprolactone-co-Lactide-co-glycolide) Nanofiber Sheet as a Potential Drug Carrier
title_full_unstemmed Preparation of Electrospun Small Intestinal Submucosa/Poly(caprolactone-co-Lactide-co-glycolide) Nanofiber Sheet as a Potential Drug Carrier
title_short Preparation of Electrospun Small Intestinal Submucosa/Poly(caprolactone-co-Lactide-co-glycolide) Nanofiber Sheet as a Potential Drug Carrier
title_sort preparation of electrospun small intestinal submucosa/poly(caprolactone-co-lactide-co-glycolide) nanofiber sheet as a potential drug carrier
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917610/
https://www.ncbi.nlm.nih.gov/pubmed/33670418
http://dx.doi.org/10.3390/pharmaceutics13020253
work_keys_str_mv AT thaonguyenthithu preparationofelectrospunsmallintestinalsubmucosapolycaprolactonecolactidecoglycolidenanofibersheetasapotentialdrugcarrier
AT leesurha preparationofelectrospunsmallintestinalsubmucosapolycaprolactonecolactidecoglycolidenanofibersheetasapotentialdrugcarrier
AT shingiru preparationofelectrospunsmallintestinalsubmucosapolycaprolactonecolactidecoglycolidenanofibersheetasapotentialdrugcarrier
AT kangyoungji preparationofelectrospunsmallintestinalsubmucosapolycaprolactonecolactidecoglycolidenanofibersheetasapotentialdrugcarrier
AT choisangdun preparationofelectrospunsmallintestinalsubmucosapolycaprolactonecolactidecoglycolidenanofibersheetasapotentialdrugcarrier
AT kimmoonsuk preparationofelectrospunsmallintestinalsubmucosapolycaprolactonecolactidecoglycolidenanofibersheetasapotentialdrugcarrier