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Biomaterials for Three-Dimensional Cell Culture: From Applications in Oncology to Nanotechnology

Three-dimensional cell culture has revolutionized cellular biology research and opened the door to novel discoveries in terms of cellular behavior and response to microenvironment stimuli. Different types of 3D culture exist today, including hydrogel scaffold-based models, which possess a complex st...

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Detalles Bibliográficos
Autores principales: Saydé, Tarek, El Hamoui, Omar, Alies, Bruno, Gaudin, Karen, Lespes, Gaëtane, Battu, Serge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917665/
https://www.ncbi.nlm.nih.gov/pubmed/33668665
http://dx.doi.org/10.3390/nano11020481
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author Saydé, Tarek
El Hamoui, Omar
Alies, Bruno
Gaudin, Karen
Lespes, Gaëtane
Battu, Serge
author_facet Saydé, Tarek
El Hamoui, Omar
Alies, Bruno
Gaudin, Karen
Lespes, Gaëtane
Battu, Serge
author_sort Saydé, Tarek
collection PubMed
description Three-dimensional cell culture has revolutionized cellular biology research and opened the door to novel discoveries in terms of cellular behavior and response to microenvironment stimuli. Different types of 3D culture exist today, including hydrogel scaffold-based models, which possess a complex structure mimicking the extracellular matrix. These hydrogels can be made of polymers (natural or synthetic) or low-molecular weight gelators that, via the supramolecular assembly of molecules, allow the production of a reproducible hydrogel with tunable mechanical properties. When cancer cells are grown in this type of hydrogel, they develop into multicellular tumor spheroids (MCTS). Three-dimensional (3D) cancer culture combined with a complex microenvironment that consists of a platform to study tumor development and also to assess the toxicity of physico-chemical entities such as ions, molecules or particles. With the emergence of nanoparticles of different origins and natures, implementing a reproducible in vitro model that consists of a bio-indicator for nano-toxicity assays is inevitable. However, the maneuver process of such a bio-indicator requires the implementation of a repeatable system that undergoes an exhaustive follow-up. Hence, the biggest challenge in this matter is the reproducibility of the MCTS and the associated full-scale characterization of this system’s components.
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spelling pubmed-79176652021-03-02 Biomaterials for Three-Dimensional Cell Culture: From Applications in Oncology to Nanotechnology Saydé, Tarek El Hamoui, Omar Alies, Bruno Gaudin, Karen Lespes, Gaëtane Battu, Serge Nanomaterials (Basel) Review Three-dimensional cell culture has revolutionized cellular biology research and opened the door to novel discoveries in terms of cellular behavior and response to microenvironment stimuli. Different types of 3D culture exist today, including hydrogel scaffold-based models, which possess a complex structure mimicking the extracellular matrix. These hydrogels can be made of polymers (natural or synthetic) or low-molecular weight gelators that, via the supramolecular assembly of molecules, allow the production of a reproducible hydrogel with tunable mechanical properties. When cancer cells are grown in this type of hydrogel, they develop into multicellular tumor spheroids (MCTS). Three-dimensional (3D) cancer culture combined with a complex microenvironment that consists of a platform to study tumor development and also to assess the toxicity of physico-chemical entities such as ions, molecules or particles. With the emergence of nanoparticles of different origins and natures, implementing a reproducible in vitro model that consists of a bio-indicator for nano-toxicity assays is inevitable. However, the maneuver process of such a bio-indicator requires the implementation of a repeatable system that undergoes an exhaustive follow-up. Hence, the biggest challenge in this matter is the reproducibility of the MCTS and the associated full-scale characterization of this system’s components. MDPI 2021-02-13 /pmc/articles/PMC7917665/ /pubmed/33668665 http://dx.doi.org/10.3390/nano11020481 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Saydé, Tarek
El Hamoui, Omar
Alies, Bruno
Gaudin, Karen
Lespes, Gaëtane
Battu, Serge
Biomaterials for Three-Dimensional Cell Culture: From Applications in Oncology to Nanotechnology
title Biomaterials for Three-Dimensional Cell Culture: From Applications in Oncology to Nanotechnology
title_full Biomaterials for Three-Dimensional Cell Culture: From Applications in Oncology to Nanotechnology
title_fullStr Biomaterials for Three-Dimensional Cell Culture: From Applications in Oncology to Nanotechnology
title_full_unstemmed Biomaterials for Three-Dimensional Cell Culture: From Applications in Oncology to Nanotechnology
title_short Biomaterials for Three-Dimensional Cell Culture: From Applications in Oncology to Nanotechnology
title_sort biomaterials for three-dimensional cell culture: from applications in oncology to nanotechnology
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917665/
https://www.ncbi.nlm.nih.gov/pubmed/33668665
http://dx.doi.org/10.3390/nano11020481
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