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Detection of Carbapenem-Resistant Enterobacterales in Simulated Blood Culture in 15 Minutes

Bacteremia leading to sepsis and organ dysfunction is a life-threatening situation, leading to death of up to one fourth of the infected individuals around the world. One major challenge in the treatment of sepsis is the rising prevalence of antibiotic resistant bacteria, such as carbapenem-resistan...

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Autores principales: Baer, Daria, Azrad, Maya, Saleh, Nora, Peretz, Avi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917675/
https://www.ncbi.nlm.nih.gov/pubmed/33672877
http://dx.doi.org/10.3390/life11020145
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author Baer, Daria
Azrad, Maya
Saleh, Nora
Peretz, Avi
author_facet Baer, Daria
Azrad, Maya
Saleh, Nora
Peretz, Avi
author_sort Baer, Daria
collection PubMed
description Bacteremia leading to sepsis and organ dysfunction is a life-threatening situation, leading to death of up to one fourth of the infected individuals around the world. One major challenge in the treatment of sepsis is the rising prevalence of antibiotic resistant bacteria, such as carbapenem-resistant Enterobacterales (CRE). In recent years, several molecular assays have been developed for the detection of CRE mechanisms, enabling rapid results reporting. We evaluated the performance of the NG-Test CARBA 5 (NG Biotech) kit in detection of CRE in simulated blood cultures. Carbapenemase-producing (CP) CRE isolates (n = 38) and non-carbapenemase CRE (Non-CP) isolates (n = 10), previously identified using the routine methods practiced at the clinical microbiology laboratory of the Baruch Padeh Medical Center, Israel, were used in this analysis. Variable concentrations of the bacterial isolates were added to a suspension composed of human blood and saline, simulating the composition of a blood culture. Samples were then transferred to an anaerobic blood culture bottle and later tested with the NG-Test CARBA 5 (NG Biotech) kit, that identifies the CRE mechanism within 15 min. The NG-Test CARBA 5 kit correctly identified 43 samples (89.5%). The sensitivity and specificity of the kits were 86.8% and 100%, respectively. In conclusion, the NG-Test CARBA 5 kit is a reliable and accessible tool for the rapid diagnosis of CRE bloodstream infections.
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spelling pubmed-79176752021-03-02 Detection of Carbapenem-Resistant Enterobacterales in Simulated Blood Culture in 15 Minutes Baer, Daria Azrad, Maya Saleh, Nora Peretz, Avi Life (Basel) Article Bacteremia leading to sepsis and organ dysfunction is a life-threatening situation, leading to death of up to one fourth of the infected individuals around the world. One major challenge in the treatment of sepsis is the rising prevalence of antibiotic resistant bacteria, such as carbapenem-resistant Enterobacterales (CRE). In recent years, several molecular assays have been developed for the detection of CRE mechanisms, enabling rapid results reporting. We evaluated the performance of the NG-Test CARBA 5 (NG Biotech) kit in detection of CRE in simulated blood cultures. Carbapenemase-producing (CP) CRE isolates (n = 38) and non-carbapenemase CRE (Non-CP) isolates (n = 10), previously identified using the routine methods practiced at the clinical microbiology laboratory of the Baruch Padeh Medical Center, Israel, were used in this analysis. Variable concentrations of the bacterial isolates were added to a suspension composed of human blood and saline, simulating the composition of a blood culture. Samples were then transferred to an anaerobic blood culture bottle and later tested with the NG-Test CARBA 5 (NG Biotech) kit, that identifies the CRE mechanism within 15 min. The NG-Test CARBA 5 kit correctly identified 43 samples (89.5%). The sensitivity and specificity of the kits were 86.8% and 100%, respectively. In conclusion, the NG-Test CARBA 5 kit is a reliable and accessible tool for the rapid diagnosis of CRE bloodstream infections. MDPI 2021-02-14 /pmc/articles/PMC7917675/ /pubmed/33672877 http://dx.doi.org/10.3390/life11020145 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Baer, Daria
Azrad, Maya
Saleh, Nora
Peretz, Avi
Detection of Carbapenem-Resistant Enterobacterales in Simulated Blood Culture in 15 Minutes
title Detection of Carbapenem-Resistant Enterobacterales in Simulated Blood Culture in 15 Minutes
title_full Detection of Carbapenem-Resistant Enterobacterales in Simulated Blood Culture in 15 Minutes
title_fullStr Detection of Carbapenem-Resistant Enterobacterales in Simulated Blood Culture in 15 Minutes
title_full_unstemmed Detection of Carbapenem-Resistant Enterobacterales in Simulated Blood Culture in 15 Minutes
title_short Detection of Carbapenem-Resistant Enterobacterales in Simulated Blood Culture in 15 Minutes
title_sort detection of carbapenem-resistant enterobacterales in simulated blood culture in 15 minutes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917675/
https://www.ncbi.nlm.nih.gov/pubmed/33672877
http://dx.doi.org/10.3390/life11020145
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