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Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis

It is known that neuronal apoptosis contributes to pathology of cerebral ischemia injury. Zonisamide (ZNS) has shown anti-apoptosis effects in recent studies. The present study investigated whether the anti-apoptotic effect can account for the neuroprotective action of ZNS on cerebral ischemia. Neur...

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Autores principales: He, Junna, Zhang, Xiangjian, He, Weiliang, Xie, Yanzhao, Chen, Yanxia, Yang, Yang, Chen, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Divulgação Científica 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917778/
https://www.ncbi.nlm.nih.gov/pubmed/33656055
http://dx.doi.org/10.1590/1414-431X202010498
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author He, Junna
Zhang, Xiangjian
He, Weiliang
Xie, Yanzhao
Chen, Yanxia
Yang, Yang
Chen, Rong
author_facet He, Junna
Zhang, Xiangjian
He, Weiliang
Xie, Yanzhao
Chen, Yanxia
Yang, Yang
Chen, Rong
author_sort He, Junna
collection PubMed
description It is known that neuronal apoptosis contributes to pathology of cerebral ischemia injury. Zonisamide (ZNS) has shown anti-apoptosis effects in recent studies. The present study investigated whether the anti-apoptotic effect can account for the neuroprotective action of ZNS on cerebral ischemia. Neuronal cells were maintained under oxygen-glucose deprivation conditions to simulate cerebral ischemia and treated with ZNS simultaneously. The apoptosis of the cells and expression of apoptosis-related proteins were investigated by flow cytometry and western blot analysis, respectively. A cerebral ischemia mouse model was created via middle cerebral artery occlusion, and the mice were treated with ZNS. Neurological deficit scores and infarct volumes of the cerebral ischemia mice were measured. The apoptosis status of the neuronal cells was evaluated by TUNEL staining. In vitro, the ZNS treatment inhibited both the apoptosis of the neuronal cells and apoptosis-related protein expression (caspase-3, caspase-8, and calpain-1) induced by the oxygen-glucose deprivation. The anti-apoptosis effect of ZNS could occur through the blocking of reactive oxygen species. Moreover, ZNS treatment significantly ameliorated neurological deficits and reduced infarct volumes in the cerebral ischemia mice model. In this study, ZNS exerted neuroprotective effects by inhibition of apoptosis in neuronal cells in cerebral ischemia. Therefore, ZNS might be a promising therapy for cerebral ischemia.
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spelling pubmed-79177782021-03-10 Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis He, Junna Zhang, Xiangjian He, Weiliang Xie, Yanzhao Chen, Yanxia Yang, Yang Chen, Rong Braz J Med Biol Res Research Article It is known that neuronal apoptosis contributes to pathology of cerebral ischemia injury. Zonisamide (ZNS) has shown anti-apoptosis effects in recent studies. The present study investigated whether the anti-apoptotic effect can account for the neuroprotective action of ZNS on cerebral ischemia. Neuronal cells were maintained under oxygen-glucose deprivation conditions to simulate cerebral ischemia and treated with ZNS simultaneously. The apoptosis of the cells and expression of apoptosis-related proteins were investigated by flow cytometry and western blot analysis, respectively. A cerebral ischemia mouse model was created via middle cerebral artery occlusion, and the mice were treated with ZNS. Neurological deficit scores and infarct volumes of the cerebral ischemia mice were measured. The apoptosis status of the neuronal cells was evaluated by TUNEL staining. In vitro, the ZNS treatment inhibited both the apoptosis of the neuronal cells and apoptosis-related protein expression (caspase-3, caspase-8, and calpain-1) induced by the oxygen-glucose deprivation. The anti-apoptosis effect of ZNS could occur through the blocking of reactive oxygen species. Moreover, ZNS treatment significantly ameliorated neurological deficits and reduced infarct volumes in the cerebral ischemia mice model. In this study, ZNS exerted neuroprotective effects by inhibition of apoptosis in neuronal cells in cerebral ischemia. Therefore, ZNS might be a promising therapy for cerebral ischemia. Associação Brasileira de Divulgação Científica 2021-02-26 /pmc/articles/PMC7917778/ /pubmed/33656055 http://dx.doi.org/10.1590/1414-431X202010498 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
He, Junna
Zhang, Xiangjian
He, Weiliang
Xie, Yanzhao
Chen, Yanxia
Yang, Yang
Chen, Rong
Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis
title Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis
title_full Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis
title_fullStr Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis
title_full_unstemmed Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis
title_short Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis
title_sort neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917778/
https://www.ncbi.nlm.nih.gov/pubmed/33656055
http://dx.doi.org/10.1590/1414-431X202010498
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