Cargando…

Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism

Global warming has two dangerous global consequences for agriculture: drought, due to water scarcity, and salinization, due to the prolonged use of water containing high concentrations of salts. Since the global climate is projected to continue to change over this century and beyond, choosing salt-t...

Descripción completa

Detalles Bibliográficos
Autores principales: Bashir, Muhammad Ajmal, Silvestri, Cristian, Coppa, Eleonora, Brunori, Elena, Cristofori, Valerio, Rugini, Eddo, Ahmad, Touqeer, Hafiz, Ishfaq Ahmad, Abbasi, Nadeem Akhtar, Nawaz Shah, Muhammad Kausar, Astolfi, Stefania
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917802/
https://www.ncbi.nlm.nih.gov/pubmed/33673090
http://dx.doi.org/10.3390/plants10020350
_version_ 1783657781084028928
author Bashir, Muhammad Ajmal
Silvestri, Cristian
Coppa, Eleonora
Brunori, Elena
Cristofori, Valerio
Rugini, Eddo
Ahmad, Touqeer
Hafiz, Ishfaq Ahmad
Abbasi, Nadeem Akhtar
Nawaz Shah, Muhammad Kausar
Astolfi, Stefania
author_facet Bashir, Muhammad Ajmal
Silvestri, Cristian
Coppa, Eleonora
Brunori, Elena
Cristofori, Valerio
Rugini, Eddo
Ahmad, Touqeer
Hafiz, Ishfaq Ahmad
Abbasi, Nadeem Akhtar
Nawaz Shah, Muhammad Kausar
Astolfi, Stefania
author_sort Bashir, Muhammad Ajmal
collection PubMed
description Global warming has two dangerous global consequences for agriculture: drought, due to water scarcity, and salinization, due to the prolonged use of water containing high concentrations of salts. Since the global climate is projected to continue to change over this century and beyond, choosing salt-tolerant plants could represent a potential paramount last resort for exploiting the secondary saline soils. Olive is considered moderately resistant to soil salinity as compared to other fruit trees, and in the present study, we investigated the influence of NaCl solutions (ranging from 0 to 200 mM) in a salt-tolerant (cv Canino) and two of its transgenic lines (Canino AT17-1 and Canino AT17-2), overexpressing tobacco osmotin gene, and in a salt-sensitive (Sirole) olive cultivar. After four weeks, most of the shoots of both Canino and Sirole plants showed stunted growth and ultimate leaf drop by exposure to salt-enriched media, contrary to transgenic lines, that did not show injuries and exhibited a normal growth rate. Malondialdehyde (MDA) content was also measured as an indicator of the lipid peroxidation level. To evaluate the role of the S assimilatory pathway in alleviating the adverse effects of salt stress, thiols levels as well as extractable activities of ATP sulfurylase (ATPS) and O-acetyl serine(thiol)lyase (OASTL), the first and the last enzyme of the S assimilation pathway, respectively, have been estimated. The results have clearly depicted that both transgenic lines overexpressing osmotin gene coped with increasing levels of NaCl by the induction of S metabolism, and particularly increase in OASTL activity closely paralleled changes of NaCl concentration. Linear correlation between salt stress and OASTL activity provides evidence that the S assimilation pathway plays a key role in adaptive response of olive plants under salt stress conditions.
format Online
Article
Text
id pubmed-7917802
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79178022021-03-02 Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism Bashir, Muhammad Ajmal Silvestri, Cristian Coppa, Eleonora Brunori, Elena Cristofori, Valerio Rugini, Eddo Ahmad, Touqeer Hafiz, Ishfaq Ahmad Abbasi, Nadeem Akhtar Nawaz Shah, Muhammad Kausar Astolfi, Stefania Plants (Basel) Article Global warming has two dangerous global consequences for agriculture: drought, due to water scarcity, and salinization, due to the prolonged use of water containing high concentrations of salts. Since the global climate is projected to continue to change over this century and beyond, choosing salt-tolerant plants could represent a potential paramount last resort for exploiting the secondary saline soils. Olive is considered moderately resistant to soil salinity as compared to other fruit trees, and in the present study, we investigated the influence of NaCl solutions (ranging from 0 to 200 mM) in a salt-tolerant (cv Canino) and two of its transgenic lines (Canino AT17-1 and Canino AT17-2), overexpressing tobacco osmotin gene, and in a salt-sensitive (Sirole) olive cultivar. After four weeks, most of the shoots of both Canino and Sirole plants showed stunted growth and ultimate leaf drop by exposure to salt-enriched media, contrary to transgenic lines, that did not show injuries and exhibited a normal growth rate. Malondialdehyde (MDA) content was also measured as an indicator of the lipid peroxidation level. To evaluate the role of the S assimilatory pathway in alleviating the adverse effects of salt stress, thiols levels as well as extractable activities of ATP sulfurylase (ATPS) and O-acetyl serine(thiol)lyase (OASTL), the first and the last enzyme of the S assimilation pathway, respectively, have been estimated. The results have clearly depicted that both transgenic lines overexpressing osmotin gene coped with increasing levels of NaCl by the induction of S metabolism, and particularly increase in OASTL activity closely paralleled changes of NaCl concentration. Linear correlation between salt stress and OASTL activity provides evidence that the S assimilation pathway plays a key role in adaptive response of olive plants under salt stress conditions. MDPI 2021-02-12 /pmc/articles/PMC7917802/ /pubmed/33673090 http://dx.doi.org/10.3390/plants10020350 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bashir, Muhammad Ajmal
Silvestri, Cristian
Coppa, Eleonora
Brunori, Elena
Cristofori, Valerio
Rugini, Eddo
Ahmad, Touqeer
Hafiz, Ishfaq Ahmad
Abbasi, Nadeem Akhtar
Nawaz Shah, Muhammad Kausar
Astolfi, Stefania
Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism
title Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism
title_full Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism
title_fullStr Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism
title_full_unstemmed Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism
title_short Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism
title_sort response of olive shoots to salinity stress suggests the involvement of sulfur metabolism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917802/
https://www.ncbi.nlm.nih.gov/pubmed/33673090
http://dx.doi.org/10.3390/plants10020350
work_keys_str_mv AT bashirmuhammadajmal responseofoliveshootstosalinitystresssuggeststheinvolvementofsulfurmetabolism
AT silvestricristian responseofoliveshootstosalinitystresssuggeststheinvolvementofsulfurmetabolism
AT coppaeleonora responseofoliveshootstosalinitystresssuggeststheinvolvementofsulfurmetabolism
AT brunorielena responseofoliveshootstosalinitystresssuggeststheinvolvementofsulfurmetabolism
AT cristoforivalerio responseofoliveshootstosalinitystresssuggeststheinvolvementofsulfurmetabolism
AT ruginieddo responseofoliveshootstosalinitystresssuggeststheinvolvementofsulfurmetabolism
AT ahmadtouqeer responseofoliveshootstosalinitystresssuggeststheinvolvementofsulfurmetabolism
AT hafizishfaqahmad responseofoliveshootstosalinitystresssuggeststheinvolvementofsulfurmetabolism
AT abbasinadeemakhtar responseofoliveshootstosalinitystresssuggeststheinvolvementofsulfurmetabolism
AT nawazshahmuhammadkausar responseofoliveshootstosalinitystresssuggeststheinvolvementofsulfurmetabolism
AT astolfistefania responseofoliveshootstosalinitystresssuggeststheinvolvementofsulfurmetabolism