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Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism
Global warming has two dangerous global consequences for agriculture: drought, due to water scarcity, and salinization, due to the prolonged use of water containing high concentrations of salts. Since the global climate is projected to continue to change over this century and beyond, choosing salt-t...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917802/ https://www.ncbi.nlm.nih.gov/pubmed/33673090 http://dx.doi.org/10.3390/plants10020350 |
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author | Bashir, Muhammad Ajmal Silvestri, Cristian Coppa, Eleonora Brunori, Elena Cristofori, Valerio Rugini, Eddo Ahmad, Touqeer Hafiz, Ishfaq Ahmad Abbasi, Nadeem Akhtar Nawaz Shah, Muhammad Kausar Astolfi, Stefania |
author_facet | Bashir, Muhammad Ajmal Silvestri, Cristian Coppa, Eleonora Brunori, Elena Cristofori, Valerio Rugini, Eddo Ahmad, Touqeer Hafiz, Ishfaq Ahmad Abbasi, Nadeem Akhtar Nawaz Shah, Muhammad Kausar Astolfi, Stefania |
author_sort | Bashir, Muhammad Ajmal |
collection | PubMed |
description | Global warming has two dangerous global consequences for agriculture: drought, due to water scarcity, and salinization, due to the prolonged use of water containing high concentrations of salts. Since the global climate is projected to continue to change over this century and beyond, choosing salt-tolerant plants could represent a potential paramount last resort for exploiting the secondary saline soils. Olive is considered moderately resistant to soil salinity as compared to other fruit trees, and in the present study, we investigated the influence of NaCl solutions (ranging from 0 to 200 mM) in a salt-tolerant (cv Canino) and two of its transgenic lines (Canino AT17-1 and Canino AT17-2), overexpressing tobacco osmotin gene, and in a salt-sensitive (Sirole) olive cultivar. After four weeks, most of the shoots of both Canino and Sirole plants showed stunted growth and ultimate leaf drop by exposure to salt-enriched media, contrary to transgenic lines, that did not show injuries and exhibited a normal growth rate. Malondialdehyde (MDA) content was also measured as an indicator of the lipid peroxidation level. To evaluate the role of the S assimilatory pathway in alleviating the adverse effects of salt stress, thiols levels as well as extractable activities of ATP sulfurylase (ATPS) and O-acetyl serine(thiol)lyase (OASTL), the first and the last enzyme of the S assimilation pathway, respectively, have been estimated. The results have clearly depicted that both transgenic lines overexpressing osmotin gene coped with increasing levels of NaCl by the induction of S metabolism, and particularly increase in OASTL activity closely paralleled changes of NaCl concentration. Linear correlation between salt stress and OASTL activity provides evidence that the S assimilation pathway plays a key role in adaptive response of olive plants under salt stress conditions. |
format | Online Article Text |
id | pubmed-7917802 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79178022021-03-02 Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism Bashir, Muhammad Ajmal Silvestri, Cristian Coppa, Eleonora Brunori, Elena Cristofori, Valerio Rugini, Eddo Ahmad, Touqeer Hafiz, Ishfaq Ahmad Abbasi, Nadeem Akhtar Nawaz Shah, Muhammad Kausar Astolfi, Stefania Plants (Basel) Article Global warming has two dangerous global consequences for agriculture: drought, due to water scarcity, and salinization, due to the prolonged use of water containing high concentrations of salts. Since the global climate is projected to continue to change over this century and beyond, choosing salt-tolerant plants could represent a potential paramount last resort for exploiting the secondary saline soils. Olive is considered moderately resistant to soil salinity as compared to other fruit trees, and in the present study, we investigated the influence of NaCl solutions (ranging from 0 to 200 mM) in a salt-tolerant (cv Canino) and two of its transgenic lines (Canino AT17-1 and Canino AT17-2), overexpressing tobacco osmotin gene, and in a salt-sensitive (Sirole) olive cultivar. After four weeks, most of the shoots of both Canino and Sirole plants showed stunted growth and ultimate leaf drop by exposure to salt-enriched media, contrary to transgenic lines, that did not show injuries and exhibited a normal growth rate. Malondialdehyde (MDA) content was also measured as an indicator of the lipid peroxidation level. To evaluate the role of the S assimilatory pathway in alleviating the adverse effects of salt stress, thiols levels as well as extractable activities of ATP sulfurylase (ATPS) and O-acetyl serine(thiol)lyase (OASTL), the first and the last enzyme of the S assimilation pathway, respectively, have been estimated. The results have clearly depicted that both transgenic lines overexpressing osmotin gene coped with increasing levels of NaCl by the induction of S metabolism, and particularly increase in OASTL activity closely paralleled changes of NaCl concentration. Linear correlation between salt stress and OASTL activity provides evidence that the S assimilation pathway plays a key role in adaptive response of olive plants under salt stress conditions. MDPI 2021-02-12 /pmc/articles/PMC7917802/ /pubmed/33673090 http://dx.doi.org/10.3390/plants10020350 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bashir, Muhammad Ajmal Silvestri, Cristian Coppa, Eleonora Brunori, Elena Cristofori, Valerio Rugini, Eddo Ahmad, Touqeer Hafiz, Ishfaq Ahmad Abbasi, Nadeem Akhtar Nawaz Shah, Muhammad Kausar Astolfi, Stefania Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism |
title | Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism |
title_full | Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism |
title_fullStr | Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism |
title_full_unstemmed | Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism |
title_short | Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism |
title_sort | response of olive shoots to salinity stress suggests the involvement of sulfur metabolism |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917802/ https://www.ncbi.nlm.nih.gov/pubmed/33673090 http://dx.doi.org/10.3390/plants10020350 |
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