Acute Effects of Cheddar Cheese Consumption on Circulating Amino Acids and Human Skeletal Muscle

Cheddar cheese is a protein-dense whole food and high in leucine content. However, no information is known about the acute blood amino acid kinetics and protein anabolic effects in skeletal muscle in healthy adults. Therefore, we conducted a crossover study in which men and women (n = 24; ~27 years,...

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Autores principales: de Hart, Naomi M.M.P., Mahmassani, Ziad S., Reidy, Paul T., Kelley, Joshua J., McKenzie, Alec I., Petrocelli, Jonathan J., Bridge, Michael J., Baird, Lisa M., Bastian, Eric D., Ward, Loren S., Howard, Michael T., Drummond, Micah J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917914/
https://www.ncbi.nlm.nih.gov/pubmed/33668674
http://dx.doi.org/10.3390/nu13020614
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author de Hart, Naomi M.M.P.
Mahmassani, Ziad S.
Reidy, Paul T.
Kelley, Joshua J.
McKenzie, Alec I.
Petrocelli, Jonathan J.
Bridge, Michael J.
Baird, Lisa M.
Bastian, Eric D.
Ward, Loren S.
Howard, Michael T.
Drummond, Micah J.
author_facet de Hart, Naomi M.M.P.
Mahmassani, Ziad S.
Reidy, Paul T.
Kelley, Joshua J.
McKenzie, Alec I.
Petrocelli, Jonathan J.
Bridge, Michael J.
Baird, Lisa M.
Bastian, Eric D.
Ward, Loren S.
Howard, Michael T.
Drummond, Micah J.
author_sort de Hart, Naomi M.M.P.
collection PubMed
description Cheddar cheese is a protein-dense whole food and high in leucine content. However, no information is known about the acute blood amino acid kinetics and protein anabolic effects in skeletal muscle in healthy adults. Therefore, we conducted a crossover study in which men and women (n = 24; ~27 years, ~23 kg/m(2)) consumed cheese (20 g protein) or an isonitrogenous amount of milk. Blood and skeletal muscle biopsies were taken before and during the post absorptive period following ingestion. We evaluated circulating essential and non-essential amino acids, insulin, and free fatty acids and examined skeletal muscle anabolism by mTORC1 cellular localization, intracellular signaling, and ribosomal profiling. We found that cheese ingestion had a slower yet more sustained branched-chain amino acid circulation appearance over the postprandial period peaking at ~120 min. Cheese also modestly stimulated mTORC1 signaling and increased membrane localization. Using ribosomal profiling we found that, though both milk and cheese stimulated a muscle anabolic program associated with mTORC1 signaling that was more evident with milk, mTORC1 signaling persisted with cheese while also inducing a lower insulinogenic response. We conclude that Cheddar cheese induced a sustained blood amino acid and moderate muscle mTORC1 response yet had a lower glycemic profile compared to milk.
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spelling pubmed-79179142021-03-02 Acute Effects of Cheddar Cheese Consumption on Circulating Amino Acids and Human Skeletal Muscle de Hart, Naomi M.M.P. Mahmassani, Ziad S. Reidy, Paul T. Kelley, Joshua J. McKenzie, Alec I. Petrocelli, Jonathan J. Bridge, Michael J. Baird, Lisa M. Bastian, Eric D. Ward, Loren S. Howard, Michael T. Drummond, Micah J. Nutrients Article Cheddar cheese is a protein-dense whole food and high in leucine content. However, no information is known about the acute blood amino acid kinetics and protein anabolic effects in skeletal muscle in healthy adults. Therefore, we conducted a crossover study in which men and women (n = 24; ~27 years, ~23 kg/m(2)) consumed cheese (20 g protein) or an isonitrogenous amount of milk. Blood and skeletal muscle biopsies were taken before and during the post absorptive period following ingestion. We evaluated circulating essential and non-essential amino acids, insulin, and free fatty acids and examined skeletal muscle anabolism by mTORC1 cellular localization, intracellular signaling, and ribosomal profiling. We found that cheese ingestion had a slower yet more sustained branched-chain amino acid circulation appearance over the postprandial period peaking at ~120 min. Cheese also modestly stimulated mTORC1 signaling and increased membrane localization. Using ribosomal profiling we found that, though both milk and cheese stimulated a muscle anabolic program associated with mTORC1 signaling that was more evident with milk, mTORC1 signaling persisted with cheese while also inducing a lower insulinogenic response. We conclude that Cheddar cheese induced a sustained blood amino acid and moderate muscle mTORC1 response yet had a lower glycemic profile compared to milk. MDPI 2021-02-13 /pmc/articles/PMC7917914/ /pubmed/33668674 http://dx.doi.org/10.3390/nu13020614 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Hart, Naomi M.M.P.
Mahmassani, Ziad S.
Reidy, Paul T.
Kelley, Joshua J.
McKenzie, Alec I.
Petrocelli, Jonathan J.
Bridge, Michael J.
Baird, Lisa M.
Bastian, Eric D.
Ward, Loren S.
Howard, Michael T.
Drummond, Micah J.
Acute Effects of Cheddar Cheese Consumption on Circulating Amino Acids and Human Skeletal Muscle
title Acute Effects of Cheddar Cheese Consumption on Circulating Amino Acids and Human Skeletal Muscle
title_full Acute Effects of Cheddar Cheese Consumption on Circulating Amino Acids and Human Skeletal Muscle
title_fullStr Acute Effects of Cheddar Cheese Consumption on Circulating Amino Acids and Human Skeletal Muscle
title_full_unstemmed Acute Effects of Cheddar Cheese Consumption on Circulating Amino Acids and Human Skeletal Muscle
title_short Acute Effects of Cheddar Cheese Consumption on Circulating Amino Acids and Human Skeletal Muscle
title_sort acute effects of cheddar cheese consumption on circulating amino acids and human skeletal muscle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917914/
https://www.ncbi.nlm.nih.gov/pubmed/33668674
http://dx.doi.org/10.3390/nu13020614
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