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Androgen Affects the Inhibitory Avoidance Memory by Primarily Acting on Androgen Receptor in the Brain in Adolescent Male Rats
Adolescence is the critical postnatal stage for the action of androgen in multiple brain regions. Androgens can regulate the learning/memory functions in the brain. It is known that the inhibitory avoidance test can evaluate emotional memory and is believed to be dependent largely on the amygdala an...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918178/ https://www.ncbi.nlm.nih.gov/pubmed/33672867 http://dx.doi.org/10.3390/brainsci11020239 |
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author | Islam, Md Nabiul Sakimoto, Yuya Jahan, Mir Rubayet Miyasato, Emi Tarif, Abu Md Mamun Nozaki, Kanako Masumoto, Koh-hei Yanai, Akie Mitsushima, Dai Shinoda, Koh |
author_facet | Islam, Md Nabiul Sakimoto, Yuya Jahan, Mir Rubayet Miyasato, Emi Tarif, Abu Md Mamun Nozaki, Kanako Masumoto, Koh-hei Yanai, Akie Mitsushima, Dai Shinoda, Koh |
author_sort | Islam, Md Nabiul |
collection | PubMed |
description | Adolescence is the critical postnatal stage for the action of androgen in multiple brain regions. Androgens can regulate the learning/memory functions in the brain. It is known that the inhibitory avoidance test can evaluate emotional memory and is believed to be dependent largely on the amygdala and hippocampus. However, the effects of androgen on inhibitory avoidance memory have never been reported in adolescent male rats. In the present study, the effects of androgen on inhibitory avoidance memory and on androgen receptor (AR)-immunoreactivity in the amygdala and hippocampus were studied using behavioral analysis, Western blotting and immunohistochemistry in sham-operated, orchiectomized, orchiectomized + testosterone or orchiectomized + dihydrotestosterone-administered male adolescent rats. Orchiectomized rats showed significantly reduced time spent in the illuminated box after 30 min (test 1) or 24 h (test 2) of electrical foot-shock (training) and reduced AR-immunoreactivity in amygdala/hippocampal cornu Ammonis (CA1) in comparison to those in sham-operated rats. Treatment of orchiectomized rats with either non-aromatizable dihydrotestosterone or aromatizable testosterone were successfully reinstated these effects. Application of flutamide (AR-antagonist) in intact adolescent rats exhibited identical changes to those in orchiectomized rats. These suggest that androgens enhance the inhibitory avoidance memory plausibly by binding with AR in the amygdala and hippocampus. |
format | Online Article Text |
id | pubmed-7918178 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79181782021-03-02 Androgen Affects the Inhibitory Avoidance Memory by Primarily Acting on Androgen Receptor in the Brain in Adolescent Male Rats Islam, Md Nabiul Sakimoto, Yuya Jahan, Mir Rubayet Miyasato, Emi Tarif, Abu Md Mamun Nozaki, Kanako Masumoto, Koh-hei Yanai, Akie Mitsushima, Dai Shinoda, Koh Brain Sci Article Adolescence is the critical postnatal stage for the action of androgen in multiple brain regions. Androgens can regulate the learning/memory functions in the brain. It is known that the inhibitory avoidance test can evaluate emotional memory and is believed to be dependent largely on the amygdala and hippocampus. However, the effects of androgen on inhibitory avoidance memory have never been reported in adolescent male rats. In the present study, the effects of androgen on inhibitory avoidance memory and on androgen receptor (AR)-immunoreactivity in the amygdala and hippocampus were studied using behavioral analysis, Western blotting and immunohistochemistry in sham-operated, orchiectomized, orchiectomized + testosterone or orchiectomized + dihydrotestosterone-administered male adolescent rats. Orchiectomized rats showed significantly reduced time spent in the illuminated box after 30 min (test 1) or 24 h (test 2) of electrical foot-shock (training) and reduced AR-immunoreactivity in amygdala/hippocampal cornu Ammonis (CA1) in comparison to those in sham-operated rats. Treatment of orchiectomized rats with either non-aromatizable dihydrotestosterone or aromatizable testosterone were successfully reinstated these effects. Application of flutamide (AR-antagonist) in intact adolescent rats exhibited identical changes to those in orchiectomized rats. These suggest that androgens enhance the inhibitory avoidance memory plausibly by binding with AR in the amygdala and hippocampus. MDPI 2021-02-14 /pmc/articles/PMC7918178/ /pubmed/33672867 http://dx.doi.org/10.3390/brainsci11020239 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Islam, Md Nabiul Sakimoto, Yuya Jahan, Mir Rubayet Miyasato, Emi Tarif, Abu Md Mamun Nozaki, Kanako Masumoto, Koh-hei Yanai, Akie Mitsushima, Dai Shinoda, Koh Androgen Affects the Inhibitory Avoidance Memory by Primarily Acting on Androgen Receptor in the Brain in Adolescent Male Rats |
title | Androgen Affects the Inhibitory Avoidance Memory by Primarily Acting on Androgen Receptor in the Brain in Adolescent Male Rats |
title_full | Androgen Affects the Inhibitory Avoidance Memory by Primarily Acting on Androgen Receptor in the Brain in Adolescent Male Rats |
title_fullStr | Androgen Affects the Inhibitory Avoidance Memory by Primarily Acting on Androgen Receptor in the Brain in Adolescent Male Rats |
title_full_unstemmed | Androgen Affects the Inhibitory Avoidance Memory by Primarily Acting on Androgen Receptor in the Brain in Adolescent Male Rats |
title_short | Androgen Affects the Inhibitory Avoidance Memory by Primarily Acting on Androgen Receptor in the Brain in Adolescent Male Rats |
title_sort | androgen affects the inhibitory avoidance memory by primarily acting on androgen receptor in the brain in adolescent male rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918178/ https://www.ncbi.nlm.nih.gov/pubmed/33672867 http://dx.doi.org/10.3390/brainsci11020239 |
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