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Selection of Lactic Acid Bacteria Species and Strains for Efficient Trapping of Drosophila suzukii

SIMPLE SUMMARY: The spotted wing drosophila (SWD) is an invasive fruit fly that causes serious economic damage to many fruit crops. Monitoring is the first step for any management program to determine the characteristics of a pest. For this purpose, there are no efficient baits registered to date. C...

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Autores principales: Alawamleh, Amani, Ðurović, Gordana, Maddalena, Giuseppe, Guzzon, Raffaele, Ganassi, Sonia, Hashmi, Maaz Maqsood, Wäckers, Felix, Anfora, Gianfranco, Cristofaro, Antonio De
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918454/
https://www.ncbi.nlm.nih.gov/pubmed/33670332
http://dx.doi.org/10.3390/insects12020153
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author Alawamleh, Amani
Ðurović, Gordana
Maddalena, Giuseppe
Guzzon, Raffaele
Ganassi, Sonia
Hashmi, Maaz Maqsood
Wäckers, Felix
Anfora, Gianfranco
Cristofaro, Antonio De
author_facet Alawamleh, Amani
Ðurović, Gordana
Maddalena, Giuseppe
Guzzon, Raffaele
Ganassi, Sonia
Hashmi, Maaz Maqsood
Wäckers, Felix
Anfora, Gianfranco
Cristofaro, Antonio De
author_sort Alawamleh, Amani
collection PubMed
description SIMPLE SUMMARY: The spotted wing drosophila (SWD) is an invasive fruit fly that causes serious economic damage to many fruit crops. Monitoring is the first step for any management program to determine the characteristics of a pest. For this purpose, there are no efficient baits registered to date. Certain bacteria release chemical compounds that attract SWD. We studied the bacterial impact on the enhanced attractiveness of a commercial bait (Droskidrink(®)) under field and laboratory conditions. At first, Oenococcus oeni belonging to lactic acid bacteria (LAB) was found to release chemical compounds that were highly attractive for SWD. The attractiveness of Droskidrink(®) bait was increased by O. oeni culture, resulting in a higher capture rate of SWD in traps. Therefore, our findings suggest the use of the bacterial culture inside the commercial SWD baits. The use of these kinds of baits can minimize the risk of pest outbreaks in fruit orchards in both domestic and wild environments. Our pest management approach is farmer-friendly in all aspects, as well as the food sector. ABSTRACT: (1) Monitoring of Drosophila suzukii is based on the use of effective traps and baits. The current baits are insufficient to provide efficient monitoring. The use of bacteria as bio-catalyzers to produce bioactive volatiles may improve flies’ attraction. Thus, we conducted this work to improve Droskidrink(®) bait’s attractiveness using lactic acid bacteria. (2) Different baits that were based on the use of Droskidrink(®) were assessed for flies’ attraction in a Droso-Trap(®) in a vineyard. Oenococcus oeni, Pediococcus spp., and Lactobacillus spp. were used. The performance of the most attractive species, O. oeni, inoculated into Droskidrink(®) was assessed in laboratory tests. The responses of female flies to volatiles produced by Droskidrink(®) with O. oeni strains were recorded by electroantennography. (3) Preliminary field assessment of baits recorded O. oeni as the most attractive species. Three strain groups showed adaptation to test conditions. Volatiles extracted by the headspace of baits inoculated with O. oeni, elicited electroantennographic responses from fly antennae. (4) Droskidrink(®) inoculated with O. oeni is a highly attractive bait for monitoring. These findings will be useful for improving the attractiveness of D. suzukii commercial baits based on the utilization of LAB volatiles in a strain-dependent manner.
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spelling pubmed-79184542021-03-02 Selection of Lactic Acid Bacteria Species and Strains for Efficient Trapping of Drosophila suzukii Alawamleh, Amani Ðurović, Gordana Maddalena, Giuseppe Guzzon, Raffaele Ganassi, Sonia Hashmi, Maaz Maqsood Wäckers, Felix Anfora, Gianfranco Cristofaro, Antonio De Insects Article SIMPLE SUMMARY: The spotted wing drosophila (SWD) is an invasive fruit fly that causes serious economic damage to many fruit crops. Monitoring is the first step for any management program to determine the characteristics of a pest. For this purpose, there are no efficient baits registered to date. Certain bacteria release chemical compounds that attract SWD. We studied the bacterial impact on the enhanced attractiveness of a commercial bait (Droskidrink(®)) under field and laboratory conditions. At first, Oenococcus oeni belonging to lactic acid bacteria (LAB) was found to release chemical compounds that were highly attractive for SWD. The attractiveness of Droskidrink(®) bait was increased by O. oeni culture, resulting in a higher capture rate of SWD in traps. Therefore, our findings suggest the use of the bacterial culture inside the commercial SWD baits. The use of these kinds of baits can minimize the risk of pest outbreaks in fruit orchards in both domestic and wild environments. Our pest management approach is farmer-friendly in all aspects, as well as the food sector. ABSTRACT: (1) Monitoring of Drosophila suzukii is based on the use of effective traps and baits. The current baits are insufficient to provide efficient monitoring. The use of bacteria as bio-catalyzers to produce bioactive volatiles may improve flies’ attraction. Thus, we conducted this work to improve Droskidrink(®) bait’s attractiveness using lactic acid bacteria. (2) Different baits that were based on the use of Droskidrink(®) were assessed for flies’ attraction in a Droso-Trap(®) in a vineyard. Oenococcus oeni, Pediococcus spp., and Lactobacillus spp. were used. The performance of the most attractive species, O. oeni, inoculated into Droskidrink(®) was assessed in laboratory tests. The responses of female flies to volatiles produced by Droskidrink(®) with O. oeni strains were recorded by electroantennography. (3) Preliminary field assessment of baits recorded O. oeni as the most attractive species. Three strain groups showed adaptation to test conditions. Volatiles extracted by the headspace of baits inoculated with O. oeni, elicited electroantennographic responses from fly antennae. (4) Droskidrink(®) inoculated with O. oeni is a highly attractive bait for monitoring. These findings will be useful for improving the attractiveness of D. suzukii commercial baits based on the utilization of LAB volatiles in a strain-dependent manner. MDPI 2021-02-11 /pmc/articles/PMC7918454/ /pubmed/33670332 http://dx.doi.org/10.3390/insects12020153 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alawamleh, Amani
Ðurović, Gordana
Maddalena, Giuseppe
Guzzon, Raffaele
Ganassi, Sonia
Hashmi, Maaz Maqsood
Wäckers, Felix
Anfora, Gianfranco
Cristofaro, Antonio De
Selection of Lactic Acid Bacteria Species and Strains for Efficient Trapping of Drosophila suzukii
title Selection of Lactic Acid Bacteria Species and Strains for Efficient Trapping of Drosophila suzukii
title_full Selection of Lactic Acid Bacteria Species and Strains for Efficient Trapping of Drosophila suzukii
title_fullStr Selection of Lactic Acid Bacteria Species and Strains for Efficient Trapping of Drosophila suzukii
title_full_unstemmed Selection of Lactic Acid Bacteria Species and Strains for Efficient Trapping of Drosophila suzukii
title_short Selection of Lactic Acid Bacteria Species and Strains for Efficient Trapping of Drosophila suzukii
title_sort selection of lactic acid bacteria species and strains for efficient trapping of drosophila suzukii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918454/
https://www.ncbi.nlm.nih.gov/pubmed/33670332
http://dx.doi.org/10.3390/insects12020153
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