Expression of Apoptosis-Related Genes in Cat Testicular Tissue in Relation to Sperm Morphology and Seasonality—A Preliminary Study

SIMPLE SUMMARY: Programmed cell death (apoptosis) is a crucial process in spermatogenesis, responsible for the elimination of abnormal sperm cells and for the reduction of testicular volume outside the breeding season. Poor sperm morphology (teratozoospermia) and lower semen quality out of season are commonly observed in domestic cats, but the exact reasons and mechanisms are not known. The aim of this study was to use gene expression analysis to identify which apoptotic processes and pathways are expressed in the phenomenon of teratozoospermia and seasonality in the domestic cat. The results showed a higher expression of two antiapoptotic genes and one proapoptotic gene during the non-reproductive season, with no differences noted between normozoospermic and teratozoospermic cats. We hypothesize that during the non-breeding season there is a potential detrimental factor which activates a cascade of caspases, against which germ cells mount a defense by producing anti-apoptotic proteins. Further identification of this factor may help in the amelioration of semen quality of cats and improve feline breeding. ABSTRACT: Apoptosis is a crucial process in spermatogenesis, responsible for the elimination of abnormal sperm cells and testicular regression out of breeding season. The aim of this study was to assess if the expression of apoptosis-related genes in testicular tissue of domestic cats differed: (1) between normozoospermic and teratozoospermic donors, and (2) between reproductive and non-reproductive season. The expression of genes: BCL2L1, BCL2, BAX, BAD, FAS, FASLG, and caspases (CASP3, CASP8, CASP9, and CASP10) was analyzed by qRT-PCR in testicular tissue samples. During non-reproductive season significantly higher expression of two anti-apoptotic genes (BCL2L1 and BCL2) was observed. Additionally, there was a significant higher expression of CASP10 in teratozoospermic cats during non-reproductive than during reproductive season. No differences were noted between normozoospermic and teratozoospermic groups. Upregulation of some genes during the non-reproductive season indicates engagement of apoptotic mechanisms in the seasonal changes of semen quality in cats, however further studies on protein levels and analysis of changes on distinct testicular germinal layers are required. At the same time, teratozoospermia in the general population of cats seems to be not connected with dysregulation of apoptosis in the testes.