Development of qPCR Detection Assay for Potato Pathogen Pectobacterium atrosepticum Based on a Unique Target Sequence

The recent taxonomic diversification of bacterial genera Pectobacterium and Dickeya, which cause soft rot in plants, focuses attention on the need for improvement of existing methods for the detection and differentiation of these phytopathogens. This research presents a whole genome-based approach t...

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Detalles Bibliográficos
Autores principales: Lukianova, Anna A., Evseev, Peter V., Stakheev, Alexander A., Kotova, Irina B., Zavriev, Sergey K., Ignatov, Alexander N., Miroshnikov, Konstantin A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918688/
https://www.ncbi.nlm.nih.gov/pubmed/33668425
http://dx.doi.org/10.3390/plants10020355
Descripción
Sumario:The recent taxonomic diversification of bacterial genera Pectobacterium and Dickeya, which cause soft rot in plants, focuses attention on the need for improvement of existing methods for the detection and differentiation of these phytopathogens. This research presents a whole genome-based approach to the selection of marker sequences unique to particular species of Pectobacterium. The quantitative real-time PCR assay developed is selective in the context of all tested Pectobacterium atrosepticum strains and is able to detect fewer than 10(2) copies of target DNA per reaction. The presence of plant DNA extract did not affect the sensitivity of the assay.

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