Development of qPCR Detection Assay for Potato Pathogen Pectobacterium atrosepticum Based on a Unique Target Sequence

The recent taxonomic diversification of bacterial genera Pectobacterium and Dickeya, which cause soft rot in plants, focuses attention on the need for improvement of existing methods for the detection and differentiation of these phytopathogens. This research presents a whole genome-based approach t...

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Autores principales: Lukianova, Anna A., Evseev, Peter V., Stakheev, Alexander A., Kotova, Irina B., Zavriev, Sergey K., Ignatov, Alexander N., Miroshnikov, Konstantin A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918688/
https://www.ncbi.nlm.nih.gov/pubmed/33668425
http://dx.doi.org/10.3390/plants10020355
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author Lukianova, Anna A.
Evseev, Peter V.
Stakheev, Alexander A.
Kotova, Irina B.
Zavriev, Sergey K.
Ignatov, Alexander N.
Miroshnikov, Konstantin A.
author_facet Lukianova, Anna A.
Evseev, Peter V.
Stakheev, Alexander A.
Kotova, Irina B.
Zavriev, Sergey K.
Ignatov, Alexander N.
Miroshnikov, Konstantin A.
author_sort Lukianova, Anna A.
collection PubMed
description The recent taxonomic diversification of bacterial genera Pectobacterium and Dickeya, which cause soft rot in plants, focuses attention on the need for improvement of existing methods for the detection and differentiation of these phytopathogens. This research presents a whole genome-based approach to the selection of marker sequences unique to particular species of Pectobacterium. The quantitative real-time PCR assay developed is selective in the context of all tested Pectobacterium atrosepticum strains and is able to detect fewer than 10(2) copies of target DNA per reaction. The presence of plant DNA extract did not affect the sensitivity of the assay.
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spelling pubmed-79186882021-03-02 Development of qPCR Detection Assay for Potato Pathogen Pectobacterium atrosepticum Based on a Unique Target Sequence Lukianova, Anna A. Evseev, Peter V. Stakheev, Alexander A. Kotova, Irina B. Zavriev, Sergey K. Ignatov, Alexander N. Miroshnikov, Konstantin A. Plants (Basel) Article The recent taxonomic diversification of bacterial genera Pectobacterium and Dickeya, which cause soft rot in plants, focuses attention on the need for improvement of existing methods for the detection and differentiation of these phytopathogens. This research presents a whole genome-based approach to the selection of marker sequences unique to particular species of Pectobacterium. The quantitative real-time PCR assay developed is selective in the context of all tested Pectobacterium atrosepticum strains and is able to detect fewer than 10(2) copies of target DNA per reaction. The presence of plant DNA extract did not affect the sensitivity of the assay. MDPI 2021-02-13 /pmc/articles/PMC7918688/ /pubmed/33668425 http://dx.doi.org/10.3390/plants10020355 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lukianova, Anna A.
Evseev, Peter V.
Stakheev, Alexander A.
Kotova, Irina B.
Zavriev, Sergey K.
Ignatov, Alexander N.
Miroshnikov, Konstantin A.
Development of qPCR Detection Assay for Potato Pathogen Pectobacterium atrosepticum Based on a Unique Target Sequence
title Development of qPCR Detection Assay for Potato Pathogen Pectobacterium atrosepticum Based on a Unique Target Sequence
title_full Development of qPCR Detection Assay for Potato Pathogen Pectobacterium atrosepticum Based on a Unique Target Sequence
title_fullStr Development of qPCR Detection Assay for Potato Pathogen Pectobacterium atrosepticum Based on a Unique Target Sequence
title_full_unstemmed Development of qPCR Detection Assay for Potato Pathogen Pectobacterium atrosepticum Based on a Unique Target Sequence
title_short Development of qPCR Detection Assay for Potato Pathogen Pectobacterium atrosepticum Based on a Unique Target Sequence
title_sort development of qpcr detection assay for potato pathogen pectobacterium atrosepticum based on a unique target sequence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918688/
https://www.ncbi.nlm.nih.gov/pubmed/33668425
http://dx.doi.org/10.3390/plants10020355
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