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Genomic and Phenotypic Analysis of Multidrug-Resistant Acinetobacter baumannii Clinical Isolates Carrying Different Types of CRISPR/Cas Systems

Acinetobacter baumannii is an opportunistic pathogen being one of the most important causative agents of a wide range of nosocomial infections associated with multidrug resistance and high mortality rate. This study presents a multiparametric and correlation analyses of clinical multidrug-resistant...

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Autores principales: Tyumentseva, Marina, Mikhaylova, Yulia, Prelovskaya, Anna, Tyumentsev, Aleksandr, Petrova, Lyudmila, Fomina, Valeria, Zamyatin, Mikhail, Shelenkov, Andrey, Akimkin, Vasiliy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918744/
https://www.ncbi.nlm.nih.gov/pubmed/33668622
http://dx.doi.org/10.3390/pathogens10020205
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author Tyumentseva, Marina
Mikhaylova, Yulia
Prelovskaya, Anna
Tyumentsev, Aleksandr
Petrova, Lyudmila
Fomina, Valeria
Zamyatin, Mikhail
Shelenkov, Andrey
Akimkin, Vasiliy
author_facet Tyumentseva, Marina
Mikhaylova, Yulia
Prelovskaya, Anna
Tyumentsev, Aleksandr
Petrova, Lyudmila
Fomina, Valeria
Zamyatin, Mikhail
Shelenkov, Andrey
Akimkin, Vasiliy
author_sort Tyumentseva, Marina
collection PubMed
description Acinetobacter baumannii is an opportunistic pathogen being one of the most important causative agents of a wide range of nosocomial infections associated with multidrug resistance and high mortality rate. This study presents a multiparametric and correlation analyses of clinical multidrug-resistant A. baumannii isolates using short- and long-read whole-genome sequencing, which allowed us to reveal specific characteristics of the isolates with different CRISPR/Cas systems. We also compared antibiotic resistance and virulence gene acquisition for the groups of the isolates having functional CRISPR/Cas systems, just CRISPR arrays without cas genes, and without detectable CRISPR spacers. The data include three schemes of molecular typing, phenotypic and genotypic antibiotic resistance determination, as well as phylogenetic analysis of full-length cas gene sequences, predicted prophage sequences and CRISPR array type determination. For the first time the differences between the isolates carrying Type I-F1 and Type I-F2 CRISPR/Cas systems were investigated. A. baumannii isolates with Type I-F1 system were shown to have smaller number of reliably detected CRISPR arrays, and thus they could more easily adapt to environmental conditions through acquisition of antibiotic resistance genes, while Type I-F2 A. baumannii might have stronger “immunity” and use CRISPR/Cas system to block the dissemination of these genes. In addition, virulence factors abaI, abaR, bap and bauA were overrepresented in A. baumannii isolates lacking CRISPR/Cas system. This indicates the role of CRISPR/Cas in fighting against phage infections and preventing horizontal gene transfer. We believe that the data presented will contribute to further investigations in the field of antimicrobial resistance and CRISPR/Cas studies.
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spelling pubmed-79187442021-03-02 Genomic and Phenotypic Analysis of Multidrug-Resistant Acinetobacter baumannii Clinical Isolates Carrying Different Types of CRISPR/Cas Systems Tyumentseva, Marina Mikhaylova, Yulia Prelovskaya, Anna Tyumentsev, Aleksandr Petrova, Lyudmila Fomina, Valeria Zamyatin, Mikhail Shelenkov, Andrey Akimkin, Vasiliy Pathogens Article Acinetobacter baumannii is an opportunistic pathogen being one of the most important causative agents of a wide range of nosocomial infections associated with multidrug resistance and high mortality rate. This study presents a multiparametric and correlation analyses of clinical multidrug-resistant A. baumannii isolates using short- and long-read whole-genome sequencing, which allowed us to reveal specific characteristics of the isolates with different CRISPR/Cas systems. We also compared antibiotic resistance and virulence gene acquisition for the groups of the isolates having functional CRISPR/Cas systems, just CRISPR arrays without cas genes, and without detectable CRISPR spacers. The data include three schemes of molecular typing, phenotypic and genotypic antibiotic resistance determination, as well as phylogenetic analysis of full-length cas gene sequences, predicted prophage sequences and CRISPR array type determination. For the first time the differences between the isolates carrying Type I-F1 and Type I-F2 CRISPR/Cas systems were investigated. A. baumannii isolates with Type I-F1 system were shown to have smaller number of reliably detected CRISPR arrays, and thus they could more easily adapt to environmental conditions through acquisition of antibiotic resistance genes, while Type I-F2 A. baumannii might have stronger “immunity” and use CRISPR/Cas system to block the dissemination of these genes. In addition, virulence factors abaI, abaR, bap and bauA were overrepresented in A. baumannii isolates lacking CRISPR/Cas system. This indicates the role of CRISPR/Cas in fighting against phage infections and preventing horizontal gene transfer. We believe that the data presented will contribute to further investigations in the field of antimicrobial resistance and CRISPR/Cas studies. MDPI 2021-02-13 /pmc/articles/PMC7918744/ /pubmed/33668622 http://dx.doi.org/10.3390/pathogens10020205 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tyumentseva, Marina
Mikhaylova, Yulia
Prelovskaya, Anna
Tyumentsev, Aleksandr
Petrova, Lyudmila
Fomina, Valeria
Zamyatin, Mikhail
Shelenkov, Andrey
Akimkin, Vasiliy
Genomic and Phenotypic Analysis of Multidrug-Resistant Acinetobacter baumannii Clinical Isolates Carrying Different Types of CRISPR/Cas Systems
title Genomic and Phenotypic Analysis of Multidrug-Resistant Acinetobacter baumannii Clinical Isolates Carrying Different Types of CRISPR/Cas Systems
title_full Genomic and Phenotypic Analysis of Multidrug-Resistant Acinetobacter baumannii Clinical Isolates Carrying Different Types of CRISPR/Cas Systems
title_fullStr Genomic and Phenotypic Analysis of Multidrug-Resistant Acinetobacter baumannii Clinical Isolates Carrying Different Types of CRISPR/Cas Systems
title_full_unstemmed Genomic and Phenotypic Analysis of Multidrug-Resistant Acinetobacter baumannii Clinical Isolates Carrying Different Types of CRISPR/Cas Systems
title_short Genomic and Phenotypic Analysis of Multidrug-Resistant Acinetobacter baumannii Clinical Isolates Carrying Different Types of CRISPR/Cas Systems
title_sort genomic and phenotypic analysis of multidrug-resistant acinetobacter baumannii clinical isolates carrying different types of crispr/cas systems
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918744/
https://www.ncbi.nlm.nih.gov/pubmed/33668622
http://dx.doi.org/10.3390/pathogens10020205
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